Pyrene-wired antibodies on highly oriented pyrolytic graphite as a label-free impedance biosensor for the sepsis biomarker procalcitonin

Mahe, L. S. A.; Green, S. J.; Winlove, C. P.; Jenkins, A. Toby A.

doi:10.1007/s10008-014-2588-7

Cited by 11 publications

(5 citation statements)

References 15 publications

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“…Life threatening conditions such as sepsis demand high quality diagnosis with fast therapeutic turnaround time (TAT). PCT sensing has been explored through various sensing platforms which includes electrochemical (Lim et al, 2017;Mahe et al, 2014;Shen et al, 2015), optical sensing methods (Baldini et al, 2009a;Baldini et al, 2009b;Vashist et al, 2016). Even commercial sensors for PCT detection exist in the market aiding in clinical decisions (Fortunato, 2016;Kutz et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor

Seshadri

Manoli

Schneiderhan‐Marra

et al. 2018

Biosensors and Bioelectronics

105

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Section: Introductionmentioning

confidence: 99%

Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor

Seshadri

Manoli

Schneiderhan‐Marra

et al. 2018

Biosensors and Bioelectronics

105

View full text Add to dashboard Cite

“…Other electrochemical sensors using highly oriented pyrolytic graphite (Mahe, Green, Winlove, & Jenkins, 2014), host‐guest nanonet electrode (Shen et al., 2015) and enzyme‐conjugated acrylic microspheres and gold nanoparticles composite coated onto a carbon‐paste screen printed electrode (Mansor et al., 2018) were also developed for sepsis biomarker detection as illustrated in Figure 9. Most recent development in electrochemical detection of sepsis in the year 2019 is the fabrication of needle‐shaped microelectrodes which can detect the levels of IL‐6 (Russell, Ward, et al, 2019), a sepsis biomarker.…”

Section: Biomarker‐based Label‐free Sepsis Diagnosismentioning

confidence: 99%

A perspective on sepsis pathogenesis, biomarkers and diagnosis: A concise survey

2020

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Sepsis is a potentially fatal physiological state caused by an imbalance in the body's immune response to an infection and is one of the most common causes for deaths in the non‐coronary intensive care unit worldwide. In this article, the state of art on sepsis is presented in a manner that facilitates easy comprehension also for the non‐medical researchers by introducing sepsis, its causes, extent and comparison of diagnostic techniques (conventional labeled as well as label‐free detection). The article also provides a comprehensive discussion on sepsis biomarkers, to help researchers from multi‐disciplinary domain in developing devices and ideas to complement the existing sepsis diagnosis systems for quick and premature detection of the physiological condition and reduce mortality by means of early treatments.

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“…Several methods have been reported for PCT determination, mainly based in the selective recognition provided by immunological interactions. These methodologies include immunoturbidimetric assays [9], chemiluminescent immunoassays [10][11][12][13][14][15][16], immunochromatographic assays [17][18][19][20][21][22], surface plasmon resonance biosensors [23][24][25], fluorescence immunosensors [26][27][28][29][30][31][32], ellipsometry immunosensors [33], colorimetric immunoassays [34][35][36], and electrochemical immunoassays [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56]. Even differen...…”

Section: Introductionmentioning

confidence: 99%

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

et al. 2020

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Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.

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Pyrene-wired antibodies on highly oriented pyrolytic graphite as a label-free impedance biosensor for the sepsis biomarker procalcitonin

Cited by 11 publications

References 15 publications

Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor

Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor

A perspective on sepsis pathogenesis, biomarkers and diagnosis: A concise survey

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

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