Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor

Seshadri, Preethi; Manoli, Kyriaki; Schneiderhan‐Marra, Nicole; Anthes, Uwe; Wierzchowiec, Piotr; Bonrad, Klaus; Franco, Cinzia Di; Torsi, Luisa

doi:10.1016/j.bios.2017.12.041

Cited by 105 publications

(95 citation statements)

References 43 publications

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“…For a stable and reproducible operation, care must be taken to make sure that the layer of biological recognition elements is covalently anchored to one of the FET electronic interfaces. Such a layer can be either attached to the gate surface, as shown in Figure a, or on the FET semiconductor (Figure b). In the former case, the biofunctionalization of the gold metal surface can be chemically engineered via thiol‐based self‐assembled monolayers (SAMs).…”

Section: The Basic Features In Organic Bioelectronic Transistor Sensorsmentioning

confidence: 99%

Section: Electrolyte‐gated Organic Field‐effect Transistor Sensorsmentioning

confidence: 99%

“…In Figure e, an EGOFET structure for the detection of procalcitonin (PCT), which is a marker for sepsis, is shown . In this example, the layer of biological recognition element (the procalcitonin specific antibody) is attached, through direct physical adsorption, to the surface of the spin‐coated P3HT organic semiconductor.…”

Section: Electrolyte‐gated Organic Field‐effect Transistor Sensorsmentioning

confidence: 99%

See 2 more Smart Citations

Ultimately Sensitive Organic Bioelectronic Transistor Sensors by Materials and Device Structure Design

Picca

Manoli

Macchia

et al. 2019

Adv Funct Materials

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140

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Organic bioelectronic sensors are gaining momentum as they can combine high-performance sensing level with flexible large-area processable materials. This opens to potentially highly powerful sensing systems for point-of-care health monitoring and diagnostics at low cost. Prominent to detect biochemical recognition events, are electrolyte-gated organic field-effect transistors (EGOFETs) and organic electrochemical transistors (OECTs) as they are easily fabricated and operated. EGOFETs are recently shown to be capable of labelfree single-molecule detections, even in serum. This progress report aims to provide a critical perspective through a selected overview of the literature on both EGOFET and OECT biosensors. Attention is paid to correctly attribute them to the potentiometric and amperometric biosensor categories, which is important to set the right conditions for quantification purposes. Moreover, to deepen the understanding of the sensing mechanisms, with the support of unpublished data, focus is put on two among the most critical aspects, namely, the capacitance interplay and the role of Faradaic currents. The final aim is to provide a rationale of the functional mechanisms encompassing both EGOFET and OECT sensors, to improve materials and devices' designs taking advantage of the processes that enhance the sensing response enabling the extremely highperformance level resulting in ultimate sensitivity, selectivity, and fast response.

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Section: The Basic Features In Organic Bioelectronic Transistor Sensorsmentioning

confidence: 99%

Section: Electrolyte‐gated Organic Field‐effect Transistor Sensorsmentioning

confidence: 99%

Section: Electrolyte‐gated Organic Field‐effect Transistor Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Ultimately Sensitive Organic Bioelectronic Transistor Sensors by Materials and Device Structure Design

Picca

Manoli

Macchia

et al. 2019

Adv Funct Materials

Self Cite

113

140

View full text Add to dashboard Cite

show abstract

“…The entire functionalization process was reported to be quick and simple, and the surface plasmon resonance results showed that the antibody coverage of CRP was effectively detected to be about 23%. Similarly, Seshadri et al [46] used a physical adsorption method to directly immobilize procalcitonin (PCT) specific Abs on the P3HT OSCs, blocked by BSA to prevent non-specific adsorption. The antibody and BSA acted as a biorecognition layer to selectively capture procalcitonin target analytes.…”

Section: Immobilization Strategies Of Antibody In Osc Layermentioning

confidence: 99%

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

Wang

Xiao

et al. 2020

Trans. Tianjin Univ.

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Organic field-effect transistors (OFETs) are fabricated using organic semiconductors (OSCs) as the active layer in the form of thin films. Due to its advantages of high sensitivity, low cost, compact integration, flexibility, and printability, OFETs have been used extensively in the sensing area. For analysis platforms, the construction of sensing layers is a key element for their efficient detection capability. The strategy used to immobilize biomolecules in these devices is especially important for ensuring that the sensing functions of the OFET are effective. Generally, analysis platforms are developed by modifying the gate/electrolyte or OSC/electrolyte interface using biomolecules, such as enzymes, antibodies, or deoxyribonucleic acid (DNA) to ensure high selectivity. To provide better or more convenient biological immobilization methods for researchers in this field and thereby improve detection sensitivity, this review summarizes recent developments in the immobilization strategies used for biological macromolecules in OFETs, including cross-linking, physical adsorption, embedding, and chemical covalent binding. The influences of biomolecules on device performance are also discussed.

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“…Poly(3-hexylthiophene) (P3HT) is one of the most used materials for this kind of devices due to its relatively high carrier mobility and easy processability [2]. Recent works have exploited P3HT as organic semiconductor for EGOFET biosensors [3], but the main drawback still affecting these devices is their fast degradation when working in ambient conditions [4], also with respect to similar devices as Organic Electro Chemical Transistors (OECTs), which exhibit a better stability in-liquid [5] and a tailorable conductive behavior [6].…”

Section: Introductionmentioning

confidence: 99%

P3HT Processing Study for In-Liquid EGOFET Biosensors: Effects of the Solvent and the Surface

Parmeggiani

Verna

Ballesio

et al. 2019

7th International Symposium on Sensor Science

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In-liquid biosensing is the new frontier of cells real time monitoring and biomarkers detection. In order to improve the stability and electrical properties of an Electrolyte Gated Organic Field Effect Transistor (EGOFET) biosensor, in this study we investigate the effect of the solvent and of the substrate modification on thin films of organic semiconductor Poly(3-hexylthiophene) (P3HT). The studied surface is the relevant interface between the P3HT and the electrolyte acting as gate dielectric for in-liquid detection of an analyte. AFM and XPS characterizations were employed to study the effect of two solvents (toluene and 1,2-dichlorobenzene) and of the adhesion promoter (Ti prime) on the morphological structure and electronic properties of P3HT film. Combining the results from the surface characterizations with electrical measurements, we investigate the changes on the EGOFET performances and stability in DI water with an Ag/AgCl gate electrode.

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Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor

Cited by 105 publications

References 43 publications

Ultimately Sensitive Organic Bioelectronic Transistor Sensors by Materials and Device Structure Design

Ultimately Sensitive Organic Bioelectronic Transistor Sensors by Materials and Device Structure Design

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

P3HT Processing Study for In-Liquid EGOFET Biosensors: Effects of the Solvent and the Surface

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