Purified Diet for Dental Caries Research with Rats

Cariogenic Potential of Liquid Medications in Rats (Short Communication)

“…3 days fol lowing birth, litters were reduced to 6 pups, with all litters providing at least 3 males for the experiment. Beginning at 18 days of age all the pups were provided diet MIT 200 [Navia, et al, 1969] and inoculat-Green wood/Feigal/Messer ed with Streptococcus mutans 6715-15. The pups were inoculated on 2 successive days.…”

Effects of Lactate Dehydrogenase on Fissure Caries in Rats

“…The sucrose content of the diet used in the main part of this study was similar in composition to the MIT-200 caries-promoting diet [Navia et al, 1969], which was used by Bibby et al [1985] who were able to show that LDH appeared to be beneficial in reducing only the number of the most severe lesions in rats. This apparent discrepancy may be attributable to dif ferences in the caries susceptibility of the rats and dif ferences in duration of experiment between the pres ent study and that of Bibby et al [1985], since our findings suggest that LDH may play a role in delaying the onset of caries lesion formation, although with in creasing duration of dietary exposure the preventive effect in less severe lesions was overcome.…”

“…More re cently, LDH has been implicated in reducing the severity of dental caries in rats [Bibby et al, 1985], The availability of suitable cariogenic diets [Navia et al, 1969] and the development of caries scoring tech niques [König et al, 1958;Green and Hartles, 1967] has enabled laboratory rats to be used extensively as reliable models for studying the effects of various fac tors on dental caries [Navia, 1977;Bowen et al, 1980;Firestone and Navia, 1988;Shern, 1988]. The purpose of this study was to determine whether the addition of LDH to high and low sucrose in the diet and drinking water may be important in reducing the number and severity of caries lesions in laboratory rats.…”

Prevention of Rat Fissure Caries by Sodium Fluoride Varnish (Duraphat®) with Different Fluoride Concentrations

“…On the 1st and 2nd days of the experi ment, the mouths of all animals were inoculated with a fresh culture of Streptococcus mutans ATCC 27351 [K-l;Fitzgerald, 1968, now known as S. sobrinus]. After weaning, all rats were fed a cariogenic diet [modified formula 200 of Navia et al, 1969] containing 49% sucrose and 18% wheat fluor. On the 1st, 2nd and 3rd day the rats were treated as follows: group 1: control, no treatment; group 2: Duraphat placebo; group 3: 2.26% Duraphat (Woelm, FRG); group 4: 1.13% Duraphat; group 5:0.56% Duraphat.…”