Purification of Fumonisin B 1 from Liquid Cultures of Fusarium proliferatum

Six 100 ppm fumonisin B(1) (FB(1)) solutions were prepared by dissolving pure standard in six different solvents containing SO(2). Two of the solvents contained 0.2 or 0.4% SO(2) in distilled water. The other four solvents were obtained by steeping corn kernels at 60 degrees C in a 0.2% SO(2) aqueous solution for 6, 12, 24, or 48 h. After the addition of FB(1), all solutions were maintained at 60 degrees C for 7 days. Fumonisin B(1) content in each solution was determined in triplicate by HPLC. Steeping corn kernels in 0.2% solution at 60 degrees C for 6 h seems to be the most effective treatment to decrease the amount of FB(1).

Section: Methodsmentioning

confidence: 99%

Fate of Fumonisin B₁ in Corn Kernel Steeping Water Containing SO₂

Pujol

Torres

Sanchis

et al. 1998

“…FB1-containing fractions, selected by TLC, were pooled and lyophilized. FB1 and HFB1 purity was evaluated by measurement of the OPA derivative as described in Dantzer et al (1996b).…”

Section: Methodsmentioning

confidence: 99%

Excretion of Fumonisin B₁, Hydrolyzed Fumonisin B₁, and the Fumonisin B₁−Fructose Adduct in Rats

Hopmans

Hauck

Hendrich

et al. 1997

Self Cite

The excretion of fumonisin B1 (FB1), hydrolyzed FB1 (HFB1), and FB1−fructose addition products (FB1−fructose) was determined in male Fisher 344/NHsd rats. Rats were dosed by gavage with 0.69, 6.93, or 69.3 μmol/kg of body weight FB1, HFB1, or FB1−fructose. Excretion of unchanged FB1, HFB1, and HFB1 after hydrolysis was determined in urine and feces by analytical reverse phase HPLC and fluorometric detection of the o-phthaldialdehyde derivatives. Average total FB1 backbone excretion in feces was 101, 76, and 50% of the dose for FB1, HFB1, and FB1−fructose, respectively. No effect of dose level was found on the percentage of the dose excreted as total FB1 after hydrolysis. FB1−fructose appears to be absorbed to the highest extent, followed by HFB1. FB1 appears to be excreted nearly completely in the feces. The greater absorption of HFB1 may explain the greater toxicity of HFB1 compared to FB1 on a molar basis. However, the detoxification of FB1 by formation of the fructose adduct cannot be explained by reduced absorption. Average total FB1 backbone excretion in urine was 2.7, 5.0, and 5.3% of the dose for FB1, HFB1, or FB1−fructose, respectively.

“…The HFB1 standard was shown to be >95% pure by FAB-mass spectrometry (Hopmans et al, 1997;Mirocha, personal communication). Residual FB1 in the 14 C-FB1-fructose dose was analyzed by HPLC (Dantzer et al, 1996b).…”

Section: Methodsmentioning

confidence: 99%

“…14 C-Fumonisin B1 purification was performed according to Dantzer et al (1996b) with modifications. Briefly, the 50 mL of 14 C-FB1-containing LM was filtered through a Whatman no.…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of 14 C-FB1 and -HFB1 were estimated by HPLC analysis of o-phthaldialdehyde derivative using a Turner fluorometer (Corning 7-60 primary filter and Wratten 2a secondary filter, 15 µL flow cell) (Dantzer et al, 1996b). The purity of 14 C-FB1 was determined by comparing the amounts as measured by HPLC with the freeze-dried mass of the purified 14 C-FB1.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Excretion of ¹⁴C-Fumonisin B₁, ¹⁴C-Hydrolyzed Fumonisin B₁, and ¹⁴C-Fumonisin B₁-Fructose in Rats

Dantzer

Hopper

Mullin

et al. 1999

Self Cite

14C-Fumonisin B(1) (FB(1)) was produced by Fusarium proliferatum M-5991 in modified Myro liquid medium and purified to >95% purity with a specific activity of 1.7 mCi/mmol. Nine male and nine female F344/N rats were each dosed by gavage with 0.69 micromol of (14)C-FB(1), (14)C-hydrolyzed FB(1), or (14)C-FB(1)-fructose/kg body weight. Urinary excretion of (14)C-FB(1) and (14)C-FB(1)-fructose was 0.5% and 4.4% of the total dose, respectively, and was similar between male and female rats. Urinary excretion of (14)C-hydrolyzed HFB(1) was significantly greater (P > 0.05) in female rats as compared with male rats (17.3% vs 12.8% of the total dose, respectively). There were no significant (P > 0.05) differences in biliary excretion of the three fumonisin compounds with a mean of 1. 4% of the dose excreted at 4 h after dosing. Lesser amounts continued to be excreted up to 9.25 h after dosing. Although biliary excretion of the (14)C-FB(1), (14)C-hydrolyzed FB(1), and (14)C-FB(1)-fructose was similar, increased urinary excretion of the (14)C-hydrolyzed FB(1) as compared to (14)C-FB(1) and (14)C-FB(1)-fructose indicated a greater absorption of the hydrolyzed form.