Purification of a novel peptide derived from Mytilus coruscus and in vitro/in vivo evaluation of its bioactive properties

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

“…Crude homogenate or mince of samples in cold water as well as pulverized, lyophilized samples, without further protein enrichment, were used for proteolysis and subsequent isolation of antioxidant peptides from the oyster [35], prawn [48], short-neck clam [34], mussels [32,44,52,53], shrimp processing waste [54], scallop female gonad [49], and sea squirt [50]. Isopropanol was used to defat the homogenates of the blood clam [33] and blue mussel [31] prior to their use in the preparation of hydrolysates.…”

“…Proteases of animal, plant, and microbial origins that are commercially available in pure form, such as pepsin, trypsin, α-chymotrypsin, papain, alcalase, and neutrase, have been used to prepare protein hydrolysates from edible marine invertebrates. Other commercial proteases that have been used, but less frequently, include kojizyme, flavourzyme, protamex, neutral protease, acid protease, newlase F, pronase and pancreatin [44,45,48,50,53,55]. …”

“…Hence, one strategy used by some studies was comparing the degree of hydrolysis (DH), yield, and/or antioxidant activity of multiple hydrolysates produced by using different proteases under optimum pH and temperature conditions. A hydrolysate was then chosen and used for the subsequent purification and identification of antioxidant peptides [31,33,41,43,44,45,48,50,53,55,56,57]. For example, Rajapakse, Mendis, Byun and Kim [43] hydrolyzed squid muscle separately with pepsin, trypsin and α-chymotrypsin for six hours.…”

“…The effectiveness of the marine invertebrate peptides in scavenging ROS, chelating metals, suppressing lipid peroxidation, and protecting cells against ROS-induced toxicity has been demonstrated by using chemical and cell-based assays [40,41,42,43]. Notably, the in vivo effects of antioxidant peptides identified from the mussel [44] and sea squirt [45] were reported, suggesting that antioxidant peptides identified from edible marine invertebrates can have biological or physiological significance. Collectively, the aforementioned findings suggest that edible marine invertebrates deserve more attention than they have received to date as a promising source of potent antioxidant peptides.…”

Improved short peptide identification using HILIC–MS/MS: Retention time prediction model based on the impact of amino acid position in the peptide sequence

“…Many studies have reported the health benefits of short food protein-derived peptides, 42 these include peptides with antioxidant, antidiabetic and antihypertensive properties (Matsui,43 Yukiyoshi, Doi, Sugimoto, Yamada & Matsumoto, 2002;Kim et al, 2013;Memarpoor-44 Yazdi, Mahaki & Zare-Zardini, 2013;Power, Jakeman & FitzGerald, 2013). One of the most 45 successful strategies for the production of bioactive peptides has been via the hydrolysis of 46 proteins.…”