Purification method development for chiral separation in supercritical fluid chromatography with the solubilities in supercritical fluid chromatographic mobile phases

Supercritical fluid chromatography in pharmaceutical analysis

“…Some other more exotic solvents (tetrahydrofuran, dichloromethane, ethyl acetate etc.) may also be employed to improve sample solubility [143], but they are only compatible with a limited number of stationary phases and their success rate is limited too. Again, because selectivity varies in an unpredictable way and since the effect of co-solvent on retention is compound and CSP-dependent, various organic solvents have to be tested during the initial screening of conditions.…”

Chiral Separations: Fundamental Review 2010

“…A comprehensive approach to the development of a purification method for SFC chiral separation for an analyte that was found to be unusually difficult to scale-up was presented, using major factors such as the solubility in mobile phases, impurity profiles, and cycle time (239).…”

Development of Mitotane Lipid Nanocarriers and Enantiomers: Two-in-One Solution to Efficiently Treat Adreno-Cortical Carcinoma

“…(E)-(Z) isomers) owing to its speed, efficiency, cost effectiveness [124,[126][127][128][129]. SFC has some significant advantages over standard HPLC methods [98,130] such as: (i) more flexibility in solvent selection; (ii) less pressure drop across the columns; (iii) faster column equilibration; (iv) faster method development; (v) higher efficiency separations and significantly less generation of hazardous waste; (vi) improving selectivity and solubility; (vii) direct mixing of polar modifiers with CO 2 . The major advantage that SFC holds over GC is the ability to separate thermally labile compounds [98,130].…”

“…SFC has some significant advantages over standard HPLC methods [98,130] such as: (i) more flexibility in solvent selection; (ii) less pressure drop across the columns; (iii) faster column equilibration; (iv) faster method development; (v) higher efficiency separations and significantly less generation of hazardous waste; (vi) improving selectivity and solubility; (vii) direct mixing of polar modifiers with CO 2 . The major advantage that SFC holds over GC is the ability to separate thermally labile compounds [98,130]. The use of SFC for the separation of enantiomers has been one of its most successful applications due to the inherent advantages of using liquid CO 2 in the mobile phase, resulting from its high diffusivity and low viscosity [131].…”