Purification de l'amylopectine. Recherches sur l'amidon 46

Meyer, Kurt H.; Gibbons, Gregory C.

doi:10.1002/hlca.19500330132

Cited by 23 publications

(5 citation statements)

References 9 publications

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“…Other sulfation procedures have involved use of S03bis(2-chloroethyl) ether at -5°for 1.5 hours for the sulfation of cellulose (247); 1,2-dichloroethane was employed as the reaction solvent. Chitosan reacted with S03 in liquid S02 for 10 to 24 hours at -10°( 514); the same system has been used for chondroitin sulfuric acid (326), and for glucosamine (327). Sulfur trioxidedioxane, used in excess, quantitatively sulfates all the hydroxyl groups in glucose and galactose and four of the hydroxyl groups in fructose at room temperature in 1 to 2 hours (485).…”

Section: Carbohydrates and Nitrogenous Polysaccharidesmentioning

confidence: 99%

The Reactions of Sulfur Trioxide, and Its Adducts, with Organic Compounds.

Gilbert¹

1962

Chem. Rev.

173

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Section: Carbohydrates and Nitrogenous Polysaccharidesmentioning

confidence: 99%

The Reactions of Sulfur Trioxide, and Its Adducts, with Organic Compounds.

Gilbert¹

1962

Chem. Rev.

173

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“…The 'active sulphate', formed enzymically under conditions similar to those described for liver, is an adenine nucleotide carrying a labile sulphate group. The rate of hydrolysis of the latter is substantially greater than that of alkyl 0or N-sulphates (Meyer & Schwartz, 1950) and is suggestive of an anhydride linkage. The substance has the same electrophoretic mobility (0.6 that of S042ions at pH 5.6) as adenosine 3'-phosphate 5'-phosphosulphate.…”

Section: Discussionmentioning

confidence: 86%

Biosynthesis of intestinal mucins. 3. Formation of ‘active sulphate’ by cell-free extracts of sheep colonic mucosa

Kent

Pasternak

1958

Biochemical Journal

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Previous results (Pasternak, Kent & Davies, 1958) showed that 35SO42ion is utilized in differing degrees by surviving intestinal tissues and that the ion is incorporated as sulphate esters of intestinal mucins (Pasternak & Kent, 1958). The incorporation is energy-dependent and inhibitors of respiration also prevent the uptake of sulphate. A relatively high level of incorporation of SO42ion was found in colonic mucosa, and on account of its availability and ease of homogenization this tissue was selected for an investigation of the individual stages of the sulphation mechanism. In liver, formation of phenolic sulphates involves the enzymic activation of SO42ions by combination with adenosine triphosphate (ATP), forming adenosine 3'-phosphate 5'-phosphosulphate (Hilz & Lipmann, 1955). D'Abramo & Lipmann (1957) secured, from chick-embryo condyles, cell-free preparationm which promote the incorporation of 35SO42ion into chondroitin sulphate, with indications that a similar sulphate-activation mechanism participates. MATERIALS AND METHODS Preparation of tissue homogenates. A portion of the large intestine (approx. 30 cm. from the anus) of adult sheep was obtained immediately after slaughter and placed in icecold saline. Within 30 min. the intestine was emptied, rinsed with water and transferred to ice-cold KCI solution (0-9 %) brought to pH 7-5 with a little 2-amino-2-hydroxymethylpropane-1:3-diol (tris). The mucosa (12-5 g.), removed by scraping with a scalpel, was immersed in 25 ml. of 0-25M-sucrose or KCI (0-9%) at 0°, eagh solution being brought to pH 8-5 by addition of tris. The tissue was then homogenized (12 strokes) in a chilled Potter-type stainlesssteel apparatus. Fractionation of homogenates. The homogenate (37 ml.) was centrifuged at 00 for 20 min. at 24 000g. The clear supernatant fraction (22 ml.) was decanted and used in the experiments described below. The precipitate was resuspended in 12 ml. of 0-25M-sucrose before investigation. Incubations. Experiments (Table 1) were performed under 02 in Warburg manometer flasks at 370 for 30-60 min., the centre well containing NaOH on filter paper. Each flask contained 0-1 m-sodium phosphate buffer (pH 7-6, 0-4 ml.), 0-2ar-MgCl2 (0-2 ml.), 0-154m-MgSO4 (0-036 ml.), 0-O2M-ATP (0-2 ml.), 10juc of Na,,35SO4 (approx. 0-1 ml.) and 0-2 ml. of a solution containing 0-5Msodium pyruvate and 0-04M-sodium fumarate. In each case 3 ml. of a suspension of mucosal scrapings or other enzyme fraction was added. In appropriate cases, 0-5msodium fluoride (0-3 ml.

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“…Foster et al 1961). On the other hand, the use of unsubstituted starting materials results in the formation of N-sulphate compounds that may also be accompanied by varying degrees of O-sulphation (see Meyer & Schwartz, 1950;Wolfrom, Shen & Summers, 1953;Ricketts, 1953;Doczi, Fischman & King, 1953;Coleman, McCarty, Warner, Willy & Flokstra, 1953;Wolfrom & Shen-Han, 1959). The sulphation conditions, based on those of Warner & Coleman (1958), used in the present study permit the selective sulphation of the amine group under conditions where O-sulphation is unlikely to occur.…”

Section: Discussionmentioning

confidence: 99%

Preparation of potassium 2-deoxy-2-[35S]sulphoamino-d-glucose

Lloyd¹,

Wusteman²,

Tudball³

et al. 1964

Biochemical Journal

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2. The isomers in the major dienoic acid fraction had c8-c8 non-conjugated configurations, and the double bonds were largely at C-11 or C-12 and C-15 or C-16. 3. The monoenoic acids formed were largely trans, with the double bond predominantly at C-13 or C-14. 4. Rapid hydrogenation of [1-14C]linoleic acid and [1-14C]oleic acid occurred in the artificial rumen. The former gave a trans monoenoic acid as an intermediary. 5. The tran8 C18 monoenoic acids passing from the rumen were almost quantitatively absorbed in the ileum. 6. The unsaturated C18 acids present in the ileum digesta were hydrogenated in the caecum and colon, so that nearly all the acids in the excreta were saturated. 7. The hydrogenation of [1-'4C]linoleic acid by caecal and colon contents resulted again in the formation of substantial amounts of trans C18 monoenoic acids. T. W. S. was in receipt of a Fellowship from the International Atomic Energy Agency during the course of this work. The Agency is also thanked for a grant towards the purchase of radioactive substrates and measuring equipment. Mr N. Huskisson and Mr N. Clarke are thanked for their valuable assistance.

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Purification de l'amylopectine. Recherches sur l'amidon 46

Cited by 23 publications

References 9 publications

The Reactions of Sulfur Trioxide, and Its Adducts, with Organic Compounds.

The Reactions of Sulfur Trioxide, and Its Adducts, with Organic Compounds.

Biosynthesis of intestinal mucins. 3. Formation of ‘active sulphate’ by cell-free extracts of sheep colonic mucosa

Preparation of potassium 2-deoxy-2-[35S]sulphoamino-d-glucose

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