Preparation of potassium 2-deoxy-2-[35S]sulphoamino-d-glucose

Abstract

2. The isomers in the major dienoic acid fraction had c8-c8 non-conjugated configurations, and the double bonds were largely at C-11 or C-12 and C-15 or C-16. 3. The monoenoic acids formed were largely trans, with the double bond predominantly at C-13 or C-14. 4. Rapid hydrogenation of [1-14C]linoleic acid and [1-14C]oleic acid occurred in the artificial rumen. The former gave a trans monoenoic acid as an intermediary. 5. The tran8 C18 monoenoic acids passing from the rumen were almost quantitatively absorbed in the ileum. 6. The unsaturated C18 acids present in the ileum digesta were hydrogenated in the caecum and colon, so that nearly all the acids in the excreta were saturated. 7. The hydrogenation of [1-'4C]linoleic acid by caecal and colon contents resulted again in the formation of substantial amounts of trans C18 monoenoic acids. T. W. S. was in receipt of a Fellowship from the International Atomic Energy Agency during the course of this work. The Agency is also thanked for a grant towards the purchase of radioactive substrates and measuring equipment. Mr N. Huskisson and Mr N. Clarke are thanked for their valuable assistance.

Infrared spectroscopy of heparins suggests that the region 750-950 cm-1 is sensitive to changes in iduronate residue ring conformation

“…N-Desulphonation together with 0-desulphation of heparin involved incubation in dimethyl sulphoxide/methanol (9:1, v/v) for 10h at 100°C (Nagasawa et al, 1977;Nagasawa & Inoue, 1980). N-Resulphonation of N-desulphonated 0-desulphated heparin involved use of a pyridine-SO3 complex (Levy & Petracek, 1962;Lloyd et al, 1964;Inoue & Nagasawa, 1973;Bruce et al, 1985). Partially carboxy-group-reduced heparin was prepared by the methods of Taylor & Conrad (1972) and Taylor et al (1980).…”

Infrared spectroscopy of chemically modified heparins

“…N-Desulphonation together with O-desulphation involved incubation in DMSO/methanol (9:1, v/v) for 10h at 100°C (Nagasawa et al, 1977;Nagasawa & Inoue, 1980). N-Resulphonation of Ndesulphonated, O-desulphated heparin involved use of a pyridine-sulphur trioxide complex; sequential incubations led to polymers that were 54, 89 and 1000% N-resulphonated (Levy & Petracek, 1962;Lloyd et al, 1964;Inoue & Nagasawa, 1973;Bruce et al, 1985). Partially (83 00)-carboxy-reduced heparin was prepared by the method of Taylor & Conrad (1972).…”

The structure of heparin oligosaccharide fragments with high anti-(factor Xa) activity containing the minimal antithrombin III-binding sequence Chemical and13C nuclear-magnetic-resonance studies

“…The yield was 20mg of heparinase from 20g of freeze-dried cells, The enzyme activity was determined as described by Linker & Hovingh (1972 N,O-Desulphated, re-N-sulphated heparin. This was prepared by selective re-N-sulphation (Lloyd et al, 1964) of N,O-desulphated heparin, prepared as described by Nagasawa et al (1977).…”