Purification, characterization and biological significance of mannose binding lectin from Dioscorea bulbifera bulbils

CAPTURE OF LECTINS FROM JACKFRUIT (Artocarpus integrifolia) SEEDS IN A SINGLE STEP USING A SUPERMACROPOROUS ION EXCHANGE CRYOGEL

“…Lectin purification from sea sponge was investigated by Marques et al (2018), who used affinity chromatography to obtain a 21-fold purification and 34% recovery. Sharma et al (2017) purified lectins from Dioscorea bulbifera by affinity techniques, also obtaining a 21-fold purification but a 16.8% recovery. Ion exchange was also used to purify lectins, with adequate results.…”

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

“…Protein purification is often tasking, time-consuming and sometimes result in the generation of unexpected results, however, attempts are being made at simplifying specific purification process taking consideration of yield, purity and biological activity of the targeted protein [51] . In recent times, diverse chromatographic techniques are being used in the purification of lectins, exploiting the size, charge, or ligand affinity of the lectin [52] , [53] , [54] , [55] . MoL was purified to homogeneity via a simple and efficient two-step purification process involving ammonium sulfate precipitation and size exclusion chromatography ( Fig.…”

Potential distributional shifts in North America of allelopathic invasive plant species under climate change models

“…For allelopathic species rich in biochemical compounds, such as those investigated here, the proposition of using bioprospecting appears viable. D. bulbifera , for instance, provides a folk remedy used in treating cancer ( Sharma et al., 2017 ). In a recent study, bulbils of D. bulbifera were isolated and purified to produce mannose-binding lectin, which could influence cancer and HIV treatment regimens ( Sharma et al., 2017 ).…”

“…D. bulbifera , for instance, provides a folk remedy used in treating cancer ( Sharma et al., 2017 ). In a recent study, bulbils of D. bulbifera were isolated and purified to produce mannose-binding lectin, which could influence cancer and HIV treatment regimens ( Sharma et al., 2017 ). S. terebinthifolia , another species with anticancer properties, similarly exhibits potentially valuable applications ( Oliveira et al., 2018 ).…”