Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

Nubi, Tolulope; Adewole, Taiwo Scholes; Agunbiade, Titilayo O.; Osukoya, Olukemi Adetutu; Kuku, Adenike

doi:10.1016/j.btre.2021.e00650

Cited by 4 publications

(3 citation statements)

References 71 publications

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“…Oxidation of exposed tryptophan at low pH is often done by the tryptophan-specific reagent N-bromosuccinimide (NBS) [ 37 , 38 ]. Titration of ProLec with aliquots of NBS at pH 6, indicated that exposed tryptophan residues represented approx 2% of the total protein (Figure 5 ), and modification of half of these tryptophans residues inactivated protein (results not shown).…”

Section: Resultsmentioning

confidence: 99%

Purification, Partial Characterization, and Evaluation of the Antiulcer Activity of Calotropis procera Leaf Lectin

Althobaiti

Konozy

2022

PPL

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Background: Lectins are proteins with therapeutic and diagnostic potential that can be applied in battling various ailments. Aim and Objective: This study was designed to purify and characterize the hemagglutinating activity derived from the leaves of Calotropis procera and its possible role in protecting the stomach against ethanol-induced lesions. Methods: The Calotropis procera leaf lectin (ProLec), was isolated by homogenization of the defatted leaf powder in Phosphate-Buffered Saline (PBS) and purified by affinity chromatography on Sephadex G-100. The lectin was eluted from the affinity column by 3% acetic acid and was physicochemically characterized. In a dose-dependent manner, ProLec was administered to rats with ethanol-induced ulcers, Biochemical, histopathological and toxicological examinations were performed. Results: ProLec is a heterodimer of 75 and 68kDa. It agglutinated all human RBCs, whereas it showed weak interaction with animal erythrocytes. The protein was optimally active at 25°C and was labile above this temperature. ProLec exhibited two pH optima and was a metalo-protein requiring Ca, Mn, and Ni. It contains 1.6% tryptophan residues of which about 1% is exposed and critical for lectin activity. The lectin exhibited a potent gastroprotective effect against ethanol-induced gastric lesions with no apparent toxicity to both kidneys and liver. Examination of the pH of the gastric juice of lectin treated animals indicated a possible role of lectin in maintaining stomach acidity within the normal ranges compared to the gastric juice pH of animals that received ethanol only. Conclusion: These results may suggest that ProLec could conceivably be a good future drug for the treatment of gastric ulcers, however, extensive immunological and toxicologically research remains needed.

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Section: Resultsmentioning

confidence: 99%

Purification, Partial Characterization, and Evaluation of the Antiulcer Activity of Calotropis procera Leaf Lectin

Althobaiti

Konozy

2022

PPL

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“…Purification was repeated several times to obtain pure ESL in sufficient quantities for antimicrobial study. The purity and molecular weight of ESL were determined using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on a 12.5% acrylamide gel using low-molecular-weight protein markers (Nubi et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Lectins are unique proteins or glycoproteins, having at least one domain devoid of catalytic activity and capable of binding specific glycan moieties reversibly (Alen’kina et al, 2014 ; Nubi et al, 2021 ). These proteins are heterogeneous in structures and proficient in agglutinating or precipitating cells and carbohydrate-relate structures via specific interactions (Singh et al, 2016 ; Adedoyin et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activities and Phylogenetic Study of Erythrina senegalensis DC (Fabaceae) Seed lectin

Enoma¹,

Adewole²,

Agunbiade³

et al. 2023

bta

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Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.

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Investigation of functional, antioxidant, anti-inflammatory, and antidiabetic properties of legume seed protein hydrolysates

Adewole,

Bieni,

Ogundepo

et al. 2024

Food Hydrocolloids for Health

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Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

Cited by 4 publications

References 71 publications

Purification, Partial Characterization, and Evaluation of the Antiulcer Activity of Calotropis procera Leaf Lectin

Purification, Partial Characterization, and Evaluation of the Antiulcer Activity of Calotropis procera Leaf Lectin

Antimicrobial Activities and Phylogenetic Study of Erythrina senegalensis DC (Fabaceae) Seed lectin

Investigation of functional, antioxidant, anti-inflammatory, and antidiabetic properties of legume seed protein hydrolysates

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