Purification and properties of urease from the leaf of mulberry, Morus alba

Effective Use of Plant-Derived Urease in the Field of Geoenvironmental/ Geotechnical Engineering

“…The urease isolated from chickpea seeds (Cicer arietinum L.) yields maximum activity at pH 7.2 [39]. This result is similar to those reported for urease from jack bean [40] and pigeon pea [13] but different from that isolated from mulberry leaves [15]. Maximum urease activity of chickpea seeds obtains at temperature of 55°C and beyond that the enzyme denatures Trifolium repens L.…”

“…Kharbooza and Banggi are common names for muskmelon. Recently, urease from mulberry leaves (Morus alba) has also purified and characterized [15].…”

“…Several families of common plants are very rich in urease, including some varieties of beans, melons and squash, and the pine family [13]. Seed urease has been purified to homogeneity from several leguminous plants [14], while leaf urease has also been subjected to investigations [15]. Extraction of urease enzyme from most urease containing plant species is simple and the enzyme is readily available from laboratory suppliers also.…”

Purification, characterization and immobilization of urease from Momordica charantia seeds

“…2B). The enzyme shows molecular mass of 174.5 kDa which is similar to urease from M. alba with 175 kDa [11] and suggesting that enzyme may exist as a homodimer. The molecular mass of urease from pigeon pea and watermelon were reported to be 540 kDa and 470 kDa [10].…”

Soybean (Glycine max) urease: Significance of sulfhydryl groups in urea catalysis

“…Urease is found in a wide variety of organisms including plants, fungi and bacteria [3,6,17,19]. Most of our knowledge about the molecular mechanism of ureolytic catalysis by plant ureases and the structure of active site is based on the 3-D structures of two bacterial ureases namely, Klebsiella aerogenes [7,8] and Bacillus pasteurii [1,2].…”