Purification, characterization and immobilization of urease from Momordica charantia seeds

Bankapalli, Leela Krishna; Singh, Abhay Narayan; Patra, Sanjukta; Dubey, Vikash Kumar

doi:10.1016/j.procbio.2011.03.022

Cited by 34 publications

(21 citation statements)

References 19 publications

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“…With the exception of dimeric ureases described for some plants, such as canatoxin from C. ensiformis [5], and the ureases from Morus alba [57] and Momordica charantia [58], all reported ureases share a basic trimeric state that may aggregate to form larger oligomers, as hexamers in the case of most plant ureases, in which each monomer carries one active site. For bac-teria, each unit of this trimer is usually itself a heterotrimer composed by the subunits UreA, UreB and UreC which co-align with plant ureases with over 50% identity.…”

Section: The Intricate Process Of Urease Activation -Much More Than Smentioning

confidence: 99%

Soybean Urease: Over a Hundred Years of Knowledge

Real‐Guerra¹,

Stanisçuaski²,

Carlini³

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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Section: The Intricate Process Of Urease Activation -Much More Than Smentioning

confidence: 99%

Soybean Urease: Over a Hundred Years of Knowledge

Real‐Guerra¹,

Stanisçuaski²,

Carlini³

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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“…It has a resistance modifying effect for aminoglycosides against methicillin-resistant Staphylococcus aureus 32 . The plant is a source of urease for urea determination 33 . Wan et al 34 have also found that M. charantia peroxidase can be used for biotransformation of piceatannol into antihyperglycaemic oligomeric stilbenes.…”

Section: Introductionmentioning

confidence: 99%

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Nkambo¹,

Anyama²,

Onegi³

2014

Afr H. Sci.

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Background: Momordica charantia L. is a medicinal plant commonly used in the management of diabetes mellitus. Objectives: We investigated the blood glucose lowering effect of the methanolic fruit extract of the Ugandan variety of M. charantia L. in alloxan-induced diabetic albino rats. Methods: 500g of M. charantia powder were macerated in methanol and the extract administered to two groups of alloxaninduced diabetic rats. The first group received 125mg/kg, the second 375mg/kg and a third group 7mg/ kg of metformin. A fourth group received 1ml normal saline. Fasting blood glucose (FBG) levels were measured at 0.5,1,2,3,5,8 and 12 hours and compared using one-way ANOVA. Results: There was an initial rise in FBG for 1 hour after administration of extracts followed by steep reductions. Significant reduction in FBG occurred at 2 hours for 125mg/kg of extract (-3.2%, 313±25.9 to 303±25.0mg/dL, p = 0.049), 375mg/ kg of extract (-3.9%, 356±19.7 to 342±20.3mg/dL, p = 0.001), and metformin (-2.6%, 344±21.7 to 335±21.1mg/dL, p = 0.003) when compared to normal saline. The maximum percentage reduction in FBG by both extracts occurred between 3 and 12 hours post dose. Conclusions: The methanolic fruit extract of M. charantia exhibits dose dependent hypoglycaemic activity in vivo.

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“…Of the three absorbance peaks identified and investigated to quantify the activity of the enzyme urease, the peak at 1,362 cm −1 was the most sensitive (greatest A over concentrations) and was the best indicator of urease activity in the control urease assay of Jackbean urease (Figures 1c and 2a-c). Karmali et al (2004) and Krishna et al (2011) also found that the 1,362-1,365 cm −1 bicarbonate peak was the most suitable for monitoring urea hydrolysis. The bicarbonate peak at 1,362 cm −1 was therefore the only peak selected to describe the urease activity in crop residues for this study.…”

Section: Identification Of Fti-r Peaks For Assaysmentioning

confidence: 92%

Ureolytic Activity of Soybean and Corn Residue Extracts

Frame

Ridgley

Gaasch

et al. 2014

Communications in Soil Science and Plant Analysis

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The enzyme urease is responsible for the rapid hydrolysis of urea in agroecosystems where more than 50% of the applied nitrogen (N) can be lost via ammonia volatilization. The objectives of the study were to (1) extract urease from corn (Zea mays L.) and soybean (Glycine max L.) and (2) compare the urease activity from soybean and corn residues to Jackbean (Canavalia ensiformis) urease using Fourier transform infrared spectroscopy (FTI-R). Concentrations of urea and sodium bicarbonate ranging from 0 to 200 mM were analyzed with FTI-R to determine the correlation to peak height. Bicarbonate produced the most responsive peaks to concentration at 1,362 cm −1 . Urease extracted from soybean residue was active, producing a bicarbonate peak at 1362 cm −1 , whereas no urease activity was observed in corn residue. Variation among urease activities in crop residues could suggest a need for more precise nitrogen management in conservation tillage agroecosystems to reduce ammonia volatilization.

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Purification, characterization and immobilization of urease from Momordica charantia seeds

Cited by 34 publications

References 19 publications

Soybean Urease: Over a Hundred Years of Knowledge

Soybean Urease: Over a Hundred Years of Knowledge

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Ureolytic Activity of Soybean and Corn Residue Extracts

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