1977
DOI: 10.1093/nar/4.11.4023
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Purification and properties of rabbit uterus preuteroglobin mRNA

Abstract: The mRNA for preuteroglobin, a precursor of the hormonally induced protein uteroglobin, has been partially purified from the endometrium of progesterone treated rabbits. The purification procedure starts with total endometrial polysomes and involves treatment with proteinase K and dodecylsulfate, chromatography on oligo(dT)-cellulose, sucrose density gradient centrifugation, electrophoresis in polyacrylamide gels containing dodecylsulfate, and a second absorption to oligo(dT)-cellulose. The final mRNA preparat… Show more

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Cited by 21 publications
(19 citation statements)
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“…Injection of the rabbits, preparation of the endometrium and isolation of total polysomal RNA were as previously reported [2,3]. Fractionation of endometrial polysomes into the free and membrane-bound fraction was performed by a modification of the procedure of Marcu et al [4].…”
Section: Purification Of M R N Amentioning
confidence: 99%
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“…Injection of the rabbits, preparation of the endometrium and isolation of total polysomal RNA were as previously reported [2,3]. Fractionation of endometrial polysomes into the free and membrane-bound fraction was performed by a modification of the procedure of Marcu et al [4].…”
Section: Purification Of M R N Amentioning
confidence: 99%
“…The poly(A)-containing RNA was purified from polysomal RNA by repeated (twice) chromatography on oligo(dT)-cellulose, and centrifuged through sucrose density gradients as previously reported [2]. Before each step the RNA was heated at 65°C and low salt for 5 min.…”
Section: Purification Of M R N Amentioning
confidence: 99%
See 3 more Smart Citations