Regulation of the Expression of the Uteroglobin Gene by Ovarian Hormones

Beato, Miguel; Arnemann, J.; Menne, C.; Müller, Heidrun; Suske, Guntram; Wenz, Michael

doi:10.1007/978-1-4684-4418-6_8

Cited by 19 publications

(11 citation statements)

References 113 publications

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“…In the genital tract this gene is expressed under the control of gonadal steroids and in the lung, under the control of glucocorticoids (for a review, see Beato et al, 1983). There is enough evidence in favour of a transcriptional control of uteroglobin expression by progesterone in the endometrium, but in the lung this hormone exhibits no such effect, although the corresponding receptor is present in this organ (Muller and Beato, 1980;Savouret et a!., 1980;Shen et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

The nucleotide sequences recognized by the glucocorticoid receptor in the rabbit uteroglobin gene region are located far upstream from the initiation of transcription.

Cato¹,

Geisse²,

Wenz³

et al. 1984

The EMBO Journal

116

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Although the expression of the uteroglobin gene in the lung is regulated by glucocorticoids, no binding sites for the glucocorticoid receptor are found in the promoter region nor can they be observed in the coding sequences. Instead a fragment situated 2.6 kb upstream from the start of transcription of the uteroglobin gene shows a high affinity for the receptor.Deoxyribonuclease I and methylation protection studies show three contiguous binding sites located within this fragment. All three sites show homology to the glucocorticoid receptor binding sites described for other genes. Two of them encompass the hexanucleotide 5' -TGTTCT-3', and the other binding site contains the homologue hexanucleotide, 5'-AGTCCT-3', but the contacts between the receptor and the hexanucleotides are equivalent to those found in other functional regulatory elements for glucocorticoids. These elements may therefore be responsible for the glucocorticoid regulation of uteroglobin gene expression by acting over a relatively long stretch of nucleotide sequences.

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Section: Introductionmentioning

confidence: 99%

The nucleotide sequences recognized by the glucocorticoid receptor in the rabbit uteroglobin gene region are located far upstream from the initiation of transcription.

Cato¹,

Geisse²,

Wenz³

et al. 1984

The EMBO Journal

116

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show abstract

“…(hCG), discovered for the first time by Ascham and Zondek in the urine of pregnant women in 1927, is perhaps the best example of a placental protein hormone used for pregnancy diagnosis [38] . With the advancement of biotechnological tools, hCG based pregnancy diagnosis has become the simplest, cheapest, and most commonly practiced test for humans to diagnose pregnancy as early as 8-10 days after conception.…”

Section: Jmscr Vol||05||issue||12||page 31222-31234||december 2017mentioning

confidence: 99%

Molecular Cloning and Characterization of Pregnancy Specific Protein Uteroglobin can Lead to Pregnancy Detection in Cattle- A Review

Ali¹

2017

jmscr

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“…It is possible that, as in the rat tyrosine aminotransferase gene (55), several widely spaced enhancer elements exist in the uteroglobin gene. As the gene is differentially regulated in various tissues (3,4), the different enhancers could selectively participate in the physiological regulation of the uteroglobin gene in different epithelial cells.…”

Section: Recruitment Of Nf-y Is Accompanied By Remodeling Of Nucleosomentioning

confidence: 99%

Hormone-dependent Recruitment of NF-Y to the Uteroglobin Gene Enhancer Associated with Chromatin Remodeling in Rabbit Endometrial Epithelium

Scholz

Truss

Beato

1999

Journal of Biological Chemistry

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Expression of the rabbit uteroglobin gene is hormonally induced in cells of the endometrial epithelium during the preimplantation phase of pregnancy. Here we show that progesterone activation of the gene is mediated by two clusters of hormone responsive elements located between 2.4 and 2.7 kilobase pairs upstream of the transcriptional start site. Between these two clusters, genomic footprinting studies in the intact endometrial epithelium reveal the hormone-inducible occupancy of several cis-acting elements. One of the protected elements shows sequence homology to the consensus binding site of the transcription factor NF-Y, which binds to the element in gel shift experiments. This uteroglobin Y box is essential for enhancer activity in transient transfection experiments with endometrial and non-endometrial cell lines, in accordance with the ubiquitous expression of NF-Y. To understand why binding of this ubiquitous factor to the uteroglobin Y box in endometrium depends on hormone induction, we examined the chromatin structure of the relevant gene region. In the uninduced state, the enhancer region appears to be organized into positioned nucleosomes. Upon hormone induction, this nucleosomal pattern is lost and the enhancer region becomes hypersensitive to nucleases, suggesting that a hormone-induced change in the local chromatin structure unmasks previously unaccessible binding sites for transcription factors. Our results emphasize the limitations of using transient transfection assays for the functional analysis of cis-acting elements and underline the need for including the native chromatin organization in this kind of studies.

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Regulation of the Expression of the Uteroglobin Gene by Ovarian Hormones

Cited by 19 publications

References 113 publications

The nucleotide sequences recognized by the glucocorticoid receptor in the rabbit uteroglobin gene region are located far upstream from the initiation of transcription.

The nucleotide sequences recognized by the glucocorticoid receptor in the rabbit uteroglobin gene region are located far upstream from the initiation of transcription.

Molecular Cloning and Characterization of Pregnancy Specific Protein Uteroglobin can Lead to Pregnancy Detection in Cattle- A Review

Hormone-dependent Recruitment of NF-Y to the Uteroglobin Gene Enhancer Associated with Chromatin Remodeling in Rabbit Endometrial Epithelium

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