Purification and properties of growth stage-dependent antiviral proteins from the leaves of Celosia cristata

Balasubrahmanyam, A.; Baranwal, V. K.; Lodha, M. L.; Varma, Anupam; Kapoor, H. C.

doi:10.1016/s0168-9452(99)00192-2

Cited by 52 publications

(31 citation statements)

References 20 publications

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“…Furthermore, it has been shown in Celosia cristata leaves that the level of two antiviral proteins against a systemic host-virus is higher at the pre-flowering stage than at the post-flowering stage (29). However, there is no information in the literature about whether this induction of expression of defense-related genes is a systemic response throughout the plant from leaves to roots and into the rhizosphere.…”

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confidence: 82%

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

De‐la‐Peña

Badri

Lei

et al. 2010

Journal of Biological Chemistry

107

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Proteins found in the root exudates are thought to play a role in the interactions between plants and soil organisms. To gain a better understanding of protein secretion by roots, we conducted a systematic proteomic analysis of the root exudates of Arabidopsis thaliana at different plant developmental stages. In total, we identified 111 proteins secreted by roots, the majority of which were exuded constitutively during all stages of development. However, defense-related proteins such as chitinases, glucanases, myrosinases, and others showed enhanced secretion during flowering. Defense-impaired mutants npr1-1 and NahG showed lower levels of secretion of defense proteins at flowering compared with the wild type. The flowering-defective mutants fca-1, stm-4, and co-1 showed almost undetectable levels of defense proteins in their root exudates at similar time points. In contrast, root secretions of defense-enhanced cpr5-2 mutants showed higher levels of defense proteins. The proteomics data were positively correlated with enzymatic activity assays for defense proteins and with in silico gene expression analysis of genes specifically expressed in roots of Arabidopsis. In conclusion, our results show a clear correlation between defense-related proteins secreted by roots and flowering time.The plant root system serves many roles, including anchorage and uptake of nutrients and water. The ability of roots to communicate with roots of neighboring plants and other organisms in the rhizosphere has been the focus of increasing attention (1, 2). Root secretions of secondary metabolites and volatile organic compounds have been shown to play offensive, defensive, and symbiotic roles (3-7). Likewise, the symbioses between Rhizobium species and various members of the legume family have been extensively studied and involve the initial secretion of specific flavonoids by plant roots (8 -10). Similarly, a multitrophic interaction has been uncovered, in which volatile organic compounds released by Medicago truncatula were shown to attract soil nematodes that could in turn bring Rhizobia, used as food by the nematodes, to the roots and thus facilitate the symbiotic process (11). All these interactions involve carbon-containing compounds secreted in the root exudates.Although numerous reports show changes in the protein profile of leaves and root tissues in response to wounding (12, 13), insect attack (14), fungal or oomycete infection (15-20), or the defense-related hormone jasmonic acid (21, 22), fewer reports have discussed the biological roles of proteins secreted by the roots (23-25). One recent study has shown that the composition of proteins secreted in the root exudates changes with the presence of a given microbial neighbor and that the exudation of proteins by a given bacterium is altered by the presence of a specific plant neighbor (26). These studies examined root secretion of proteins under inducible conditions, but there is no information available about the protein profiles in the root exudates under normal, nonstressed...

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confidence: 82%

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

De‐la‐Peña

Badri

Lei

et al. 2010

Journal of Biological Chemistry

107

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“…Thus, this plant is also popular as the ornamental plant for landscape, potted, and cut flowers. C. cristata is also used as a medicinal plant with several biological activities such as antioxidant activity from flower extract (Kim et al 2015b), antiviral activity from leaves extract (Balasubrahmanyam et al 2000), immunomodulatory activity from polysaccharide CP1-1 isolated (Sun et al 2015), and hepatoprotective activity from saponin and triterpenoid saponin isolated ( Wang et al 2010;Sun et al 2011). Therefore, the diversity of morphology and metabolite in C. cristata needs to be increased to maximize the potential plants used as an ornamental and herb plant.…”

Section: Introductionmentioning

confidence: 99%

The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds

et al. 2019

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Abstract. Aisyah SI, Muhallilin I, Sukma D, Nurcholis W. 2019. The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds. Biodiversitas 20: 3766-3771. The present work investigated the agro-morphological changes in Celosia cristata L. seeds exposed to gamma irradiations. This study also conducted phytochemical screening to screened secondary metabolite change in C. cristata. C. cristata seeds were irradiated with acute radiation at doses of 470, 480, and 490 Gy and were planted until M1 generation. Recurrent irradiation was done on M1 seeds at a dose of 250 Gy. The agro-morphological and phytochemical diversity was performed in M2 generation. The highest difference characters in acute irradiated plants were found on plant height, number of branches, and flower length, while the plant diameter, flower width, and number of flowers were found the highest in recurrent irradiation. Agro-morphological important changes in recurrent irradiated plants were decreased plant height and increased the number of flowers that compared with acute irradiated and control plants. The qualitative phytochemical showed no difference between acute and recurrent irradiated plants. It can be concluded that the recurrent irradiated changes the morphological valuable of C. cristata, while the phytochemical is needed further research.

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“…However, almost all of the virus‐inhibiting substances from higher plants that have been studied have been recognized as basic proteins 3. A variety of proteins with inhibitory activity against viral infection and/or replication have been isolated and purified 8–13. In addition, studies have pointed out that some natural low‐molecular‐weight compounds such as flavonoids and alkaloids also show antiphytoviral effects 14–17…”

Section: Introductionmentioning

confidence: 99%

Controllable Synthesis of Organic–Inorganic Hybrid MoO_x/Polyaniline Nanowires and Nanotubes

Wang

Gao

Zhang

et al. 2011

Chemistry A European J

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A novel chemical oxidative polymerization approach has been proposed for the controllable preparation of organic-inorganic hybrid MoO(x)/polyaniline (PANI) nanocomposites based on the nanowire precursor of Mo(3)O(10)(C(6)H(8)N)(2)·2H(2)O with sub-nanometer periodic structures. The nanotubes, nanowires, and rambutan-like nanoparticles of MoO(x)/PANI were successfully obtained through simply modulating the pH values to 2.5-3.5, ≈2.0 and ≈1.0, respectively. Through systematic physicochemical characterization, such as scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and so forth, the composition and structure of MoO(x)/PANI hybrid nanocomposites are well confirmed. It is found that the nanowire morphology of the precursor is the key to achieve the one-dimensional (1D) structures of final products. A new polymerization-dissolution mechanism is proposed to explain the formation of such products with different morphologies, in which the match between polymerization and dissolution processes of the precursor plays the important role. This approach will find a new way to controllably prepare various organic-inorganic hybrid 1D nanomaterials especially for polymer-hybrid nanostructures.

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Purification and properties of growth stage-dependent antiviral proteins from the leaves of Celosia cristata

Cited by 52 publications

References 20 publications

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds

Controllable Synthesis of Organic–Inorganic Hybrid MoO_x/Polyaniline Nanowires and Nanotubes

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Purification and properties of growth stage-dependent antiviral proteins from the leaves of Celosia cristata

Cited by 52 publications

References 20 publications

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds

Controllable Synthesis of Organic–Inorganic Hybrid MoOx/Polyaniline Nanowires and Nanotubes

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Controllable Synthesis of Organic–Inorganic Hybrid MoO_x/Polyaniline Nanowires and Nanotubes