Purification and Characterization of the Acid Soluble 26-kDa Polypeptide from Soybean Seeds

Peptide Mapping and Assessment of Cryoprotective Activity of 26/27-kDa Dehydrin from Soybean Seeds

“…In the electrophoretic development, three bands of fragments were detected on the PVDF membrane, similarly to the previous report 2) (data not shown). The partial peptide sequence of a digested fragment was found to be identical to the Nterminal DEYGNP conservative motif of Mat9, and another peptide was attributed to a region between the internal glycine-rich region and C-terminal lysine motif (Fig.…”

“…The puriˆcation procedure for dehydrin reported previously 2) was modiˆed by introducing a heating process after ammonium sulfate fractionation to completely remove the basic 7S globulin which was composed of 16-kDa and 27-kDa subunits. 5) Since this heating procedure facilitated the removal of other heat-sensitive polypeptides, dehydrins were puriˆed through the following ion-exchange chromatography and hydrophobic interaction chromatography to one band on SDS-PAGE (Fig.…”

Plant dehydrins — Tissue location, structure and function

“…Carrot ECP40 (YSK 2 ) was found to localize in the endosperm and zygotic embryos in mature seeds [29]. Other dehydrins, such as a 24-kD (MAT1; YK) and a 26/27-kD (MAT9; YK) were purified from mature soybean (Glycine max) seeds [30,31]; a 35-kDa dehydrin (Y 2 K) was purified from dry seeds of cowpea (Vigna unguiculata) [22]. Most of the known dehydrins are localized in vegetative tissues under normal growth conditions, and in floral organs.…”

“…For example, six Dhn genes have been characterized in Arabidopsis [23], thirteen in Hordeum vulgare [24,25], and at least two in many other plant species [26, UniProt Knowledgebase, http://www.expasy.org/cgi-bin/]. Immunohistochemical and fractionation studies revealed that dehydrins are not only accumulated under the conditions of water deficit that occur during the late stages of embryogenesis [2,[27][28][29][30][31] and in vegetative tissues in response to stress leading to cellular dehydration such as drought, low temperature and salinity [1,2,14,21,22,[32][33][34][35][36], but they are also present in nearly all vegetative tissues during normal growth conditions [25,32,34,37,38]. Such a wide distribution of dehydrins in various tissues during plant growth and in response to stress leading to cellular dehydration suggests that these proteins may play an essential role in plant growth and in stress tolerance.…”

A Pepsin-Resistant 20 kDa Protein Found in Red Kidney Bean (Phaseolus vulgarisL.) Identified as Basic Subunit of Legumin

“…3) The disulfide bonds in the bean proteins were examined by the fluorescent labeling method. 4) Bean flour (200 mg) was mixed with 2 ml of extraction buffer (30 mM Tris-HCl, pH 7.9, containing 1 mM PMSF and 0.02% NaN 3 ), and centrifuged at 10;000 Â g for 15 min.…”