A Pepsin-Resistant 20 kDa Protein Found in Red Kidney Bean (Phaseolus vulgarisL.) Identified as Basic Subunit of Legumin

Electrophoretic Profiles and Angiotensin I-Converting Enzyme Inhibitory Activities of Nine Varieties of Phaseolus Vulgaris Protein Hydrolysates

“…Various workers have indicated that the predominant protein in dry beans, i.e. phaseolin, which has a MM of around 47 kDa, has a compact structure and high β-sheet conformation which makes it resistant to peptic digestion and partially resistant to trypsin and α-chymotrypsin digestion [12,18,19]. This is consistent with our findings.…”

Allergenic responses of red kidney bean (Phaseolus vulgaris cv chitra) polypeptides in BALB/c mice recognized by bronchial asthma and allergic rhinitis patients

“…IgE antibodies were detected against RKB, white bean, pinto bean, chickpea, pea and black-eyed in human (Zacharisen & Kurup, 1998). Pepsin resistant proteins have also been reported in RKB by some groups using simulated gastric fluid (SGF) assay (Misra, Prasad, Das, & Dwivedi, 2009;Moma, 2006) but their purification and characterization is not well established.…”

Characterization of a Novel Legumin α-Amylase Inhibitor from Chickpea (Cicer arietinumL.) Seeds

“…According to the UniProt Knowledgebase, the legumin (Q9SMJ4) from Cicer arietinum consists of alpha and beta subunits, but no property of enzyme activity has been reported for it. To our knowledge, a basic subunit of legumin in the red kidney bean has been reported to have proteinase inhibitory activity, 19) but no legumin has been reported to have -amylase inhibitory activity. This suggests that CLAI is the first legumin reported to have -amylase inhibitory activity.…”