Purification and Characterization of the Platynota idaeusalis Baculovirus Pathogenic to the Tufted Apple Bud Moth

P34.8 (GP37) is not essential for baculovirus replication

“…Inactivation of p34.8 offers the possibility of investigating the effect on infectivity of OBs to larvae or on the kinetics of virus replication in tissue culture. The LD 50 of purified Acp34.8 − OBs was determined in third-instar T. ni larvae by using the diet plug method (Wang & McCathy, 1993 ). Parental AcUW1-LacZ was used as a control.…”

“…OBs of AcUW1-LacZ and pAcp34.8 − were purified by differential and sucrose gradient centrifugation and suspended in distilled water at a concentration of 3×10 6 OBs/ml (Cheng et al ., 1990 ). Samples containing 1·5×10 4 , 3×10 4 , 3×10 5 , 1·6×10 6 and 3×10 6 OBs/ml were used to determine the LD 50 in larvae (Wang & McCathy, 1993 ). Briefly, 1 μl of virus suspension was applied onto a small diet plug and third-instar Trichoplusia ni larvae (50 animals per sample) were allowed to feed until all the diet was consumed, before transfer onto fresh diet.…”

Biological and molecular characterization of a multicapsid nucleopolyhedrovirus from Thysanoplusia orichalcea (L.) (Lepidoptera: Noctuidae)

“…PuriWcation of virions of baculoviruses is mostly accomplished by sucrose gradient centrifugation (Harrap et al, 1977;Kawanishi and Paschke, 1970;Wang and McCarthy, 1993). The number of bands formed by an NPV depends on the maximum number of nucleocapsids per envelope (Kawanishi and Paschke, 1970).…”

Polyhedrin sequence determines the tetrahedral shape of occlusion bodies in Thysanoplusia orichalcea single-nucleocapsid nucleopolyhedrovirus.

“…Because the soybean looper colony was contaminated with a cypovirus, an alternative species, beet armyworm, was used for the infectivity test. The method for bioassay followed Wang & McCarthy (1993) with the following modification. Lettuce leaf disks (4 mm) were substituted for the diet disks (Wang & McCarthy, 1993) and 50 third-instar beet armyworms were given each virus dose.…”

“…The method for bioassay followed Wang & McCarthy (1993) with the following modification. Lettuce leaf disks (4 mm) were substituted for the diet disks (Wang & McCarthy, 1993) and 50 third-instar beet armyworms were given each virus dose. The doses were 0n29, 2n9, 29, 290 and 2900 OBs per larva for AcMNPV, and 0n36, 3n6, 36n6, 366 and 3660 OBs per larva for RECAcV.…”