Biological and molecular characterization of a multicapsid nucleopolyhedrovirus from Thysanoplusia orichalcea (L.) (Lepidoptera: Noctuidae)

Cheng, Xin; Carner, G. R.; Lange, Martin; Jehle, Johannes A.; Arif, Basil M.

doi:10.1016/j.jip.2004.12.003

Cited by 22 publications

(16 citation statements)

References 32 publications

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“…All cells were maintained in Grace's medium (Invitrogen) supplemented with 10 % fetal bovine serum at 27°C. Recombinant baculoviruses were generated from AcMNPV (Invitrogen), ThorMNPV [6] and SeMNPV as indicated below. ThorMNPV and SeMNPV formed polyhedra, while recombinant AcMNPVs did not.…”

Section: Cell Lines and Virusesmentioning

confidence: 99%

“…It was found that Autographa californica M nucleopolyhedrovirus (AcMNPV) (one of the most extensively studied viruses, of the family Baculoviridae) has a wide host range and infects insects of more than 33 species and successfully replicates in many cell lines [5]. On the other hand, narrow-host-range NPVs, such as Thysanoplusia orichalcea MNPV (ThorMNPV) and Spodoptera exigua MNPV (SeMNPV), successfully infect and replicate in only a few cell lines [6][7][8][9]. In nature, an insect can also be infected by more than one NPV simultaneously, resulting in a mixed infection [6,10].…”

Section: Introductionmentioning

confidence: 99%

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AcMNPV enhances infection by ThorNPV in Sf21 cells and SeMNPV in Hi5 cells

Salem

Cheng

2012

Arch Virol

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Section: Cell Lines and Virusesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

AcMNPV enhances infection by ThorNPV in Sf21 cells and SeMNPV in Hi5 cells

Salem

Cheng

2012

Arch Virol

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“…However, the images obtained by these authors do not differ from that obtained with the use of the technique three, described in this work, where the faces of the polyhedron appeared smooth, facilitating the morphologic descriptions. Similar methodology is described by Cheng et al (1998), Cheng & Carner (2000 and Cheng et al (2005), used to determine the morphology of SNPV and MNPV of Thysanoplusia orichalcea (L.) (Lepidoptera: Noctuidae).…”

Section: Resultsmentioning

confidence: 99%

Nucleopolyhedrovirus: scanning electron microscopy technique

Torquato¹,

Neto²,

Brancalhão³

et al. 2006

Neotrop. Entomol.

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Neotropical Entomology 35(6):787-790 (2006) Nucleopolyhedrovirus: Técnica de Microscopia Eletrônica de Varredura RESUMO -Um método simples foi desenvolvido para se estudar a forma geométrica do Bombyx mori Nucleopolyhedrovirus múltiplo, BmMNPV, para microscopia eletrônica de varredura. O vírus, pertencente à Família Baculoviridae, foi obtido do fi ltrado, do inóculo e da hemolinfa de lagartas de Bombyx mori (L.) (Lepidoptera: Bombycidae) infectadas. O material foi colocado diretamente sobre o suporte de varredura, onde previamente foi aderida uma fi ta de cobre. Após secagem e metalização com carbono e ouro, o vírus foi analisado ao microscópio de varredura. Os poliedros apresentaramse com as faces lisas, perfeitamente defi nidas, possibilitando a análise matemática que o identifi cou como um octaedro truncado. A forma do poliedro pode apresentar valor taxonômico, uma vez que ela é específi ca para cada linhagem viral. PALAVRAS-CHAVE: Baculovírus, Bombyx mori, BmMNPV, poliedro, octaedro truncado ABSTRACT -A simplifi ed methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the fi ltrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defi ned morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that identifi ed it as a truncated octahedron. The form of the polyhedron can present taxonomic value, once it is specifi c for each viral lineage.KEY WORDS: Baculovirus, Bombyx mori, BmMNPV, polyhedra, truncated octahedron Nucleopolyhedrovirus (NPV) is an important entomopathogenic virus belonging to Baculoviridae family. It is made up of double stranded DNA molecule, associated with proteins of the capsid, forming the nucleocapsid. Two different subgroups are recognized for NPV: the occlusion derived virions and the budded virus. These virion subgroups have different functions in the infection cycle. The occluded subgroups could be divided into two subgroups: those containing one nucleocapsid per virion are referred to as single-capsid NPVs (SNPVs), whereas those containing multiple nucleocapsids per virion are known as multiple NPVs (MNPVs) (Murphy et al. 1995, Blissard 1996.Nucleocapsid and envelope constitutes the virion or infectious particle. In NPV several virions are contained in a protein structure, named occlusion body or polyhedron (Adams & McClintock 1991, Murphy et al. 1995, Blissard 1996. Polyhedra are stable structures that can persist for long time in the environment, where they are commonly found on the surface of the plants or in the soil. In the so...

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“…A total of 300,000 PE reads were randomly selected and assembled into a single contig with Edena (3). The assembled genome sequence was confirmed by comparing three predicted restriction endonuclease (REN) profiles with the actual REN digestions (2).…”

mentioning

confidence: 99%

“…Top2 genomic DNA was extracted from budded viruses generated from Hi5 cell infections (4), and sequencing was performed with the Solexa Genome Analyzer at BGI (Beijing Genome Institution, Shenzhen, China). A total of 5,622,224 clean pair-end (PE) reads were obtained, with an average insert fragment size of 500 bp, roughly 500 million nucleotides, representing 3,800ϫ coverage of the 130-kbp ThorMNPV genome (2). A total of 300,000 PE reads were randomly selected and assembled into a single contig with Edena (3).…”

mentioning

confidence: 99%