Polyhedrin sequence determines the tetrahedral shape of occlusion bodies in Thysanoplusia orichalcea single-nucleocapsid nucleopolyhedrovirus.

Cheng, Xin; Carner, G. R.; Fescemyer, Howard W.

doi:10.1099/0022-1317-79-10-2549

Cited by 20 publications

(17 citation statements)

References 29 publications

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“…Second, mutations introduced in or near helix H1/2 affect the morphology and infectivity of polyhedra, suggesting a role for this helix in assembly and virus packaging. Mutations controlling the shape of polyhedra were previously identified at residues 24, 41, and 58 (mutations were reported as G25D (11), L43I (14), and P58L (15) in the original numbering). These mutations result in a change of the crystal morphology from a variety of polyhedral forms to cuboid crystals.…”

Section: Polyhedra Consist Of Disulfide-linked Tetrahedral Clusters Omentioning

confidence: 99%

The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses

Coulibaly

Chiu

Gutmann

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Baculoviruses are ubiquitous insect viruses well known for their use as bioinsecticides, gene therapy vectors, and protein expression systems. Overexpression of recombinant proteins in insect cell culture utilizes the strong promoter of the polyhedrin gene. In infected larvae, the polyhedrin protein forms robust intracellular crystals called polyhedra, which protect encased virions for prolonged periods in the environment. Polyhedra are produced by two unrelated families of insect viruses, baculoviruses and cypoviruses. The atomic structure of cypovirus polyhedra revealed an intricate packing of trimers, which are interconnected by a projecting N-terminal helical arm of the polyhedrin molecule. Baculovirus and cypovirus polyhedra share nearly identical lattices, and the Nterminal region of the otherwise unrelated baculovirus polyhedrin protein sequence is also predicted to be ␣-helical. These results suggest homology between the proteins and a common structural basis for viral polyhedra. Here, we present the 2.2-Å structure of baculovirus polyhedra determined by x-ray crystallography from microcrystals produced in vivo. We show that the underlying molecular organization is, in fact, very different. Although both polyhedra have nearly identical unit cell dimensions and share I23 symmetry, the polyhedrin molecules are structurally unrelated and pack differently in the crystals. In particular, disulfide bonds and domain-swapped N-terminal domains stabilize the building blocks of baculovirus polyhedra and interlocking C-terminal arms join unit cells together. We show that the N-terminal projecting helical arms have different structural roles in baculovirus and cypovirus polyhedra and conclude that there is no structural evidence for a common evolutionary origin for both classes of polyhedra.in vivo crystallization ͉ molecular arms ͉ occlusion body ͉ self-assembly ͉ virus evolution

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Section: Polyhedra Consist Of Disulfide-linked Tetrahedral Clusters Omentioning

confidence: 99%

The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses

Coulibaly

Chiu

Gutmann

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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“…AAB31529); MbMNPV, Mamestra brassicae MNPV ; OpMNPV, Orgyia pseudotsugata MNPV (Ahrens et al, 1997); OpSNPV, Orgyia pseudotsugata SNPV (Leisy et al, 1986); PaflMNPV, Panolis flammea MNPV (Oakey et al, 1989); PenuNPV, Perina nuda NPV (Chou et al, 1996); RoMNPV, Rachiplusia ou MNPV (Harrison & Bonning, 1999); SeMNPV, Spodoptera exigua MNPV (IJkel et al, 1999); SfMNPV, Spodoptera frugiperda MNPV (Gonzalez et al, 1989); SpltMNPV, Spodoptera litura MNPV (Pang et al, 2001); ThorMNPV, Thysanoplusia orichalcea MNPV (accession no. AF169480); ThorSNPV, Thysanoplusia orichalcea SNPV (Cheng et al, 1998); TnSNPV, Trichoplusia ni SNPV (Fielding & Davison, 1999); WisiNPV, Wiseana signata NPV (accession no. AF016916); XecnGV, Xestia c-nigrum granulovirus (Hayakawa et al, 1999 (Dreschers et al, 2001).…”

Section: Comparison Of Romnpv and Acmnpv Orfsmentioning

confidence: 99%

Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses

Harrison

Bonning

2003

Journal of General Virology

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The Rachiplusia ou multiple nucleopolyhedrovirus (RoMNPV) is a variant of Autographa californica MNPV (AcMNPV) but is significantly more virulent against several major agricultural pests. The genome sequence of the R1 strain of RoMNPV was determined and compared to that of AcMNPV strain C6. The RoMNPV genome is approximately 131?5 kbp with a G+C content of 39?1 %. The homologous repeat regions (hrs) described for AcMNPV-C6 are present in RoMNPV-R1 but the hrs of RoMNPV have fewer palindromic repeats. The RoMNPV-R1 nucleotide sequence is almost completely collinear with the sequence of AcMNPV-C6 and contains homologues of 150 of the 155 ORFs described for AcMNPV-C6. Deletions, insertions and substitutions have resulted in the loss of homologues for AcMNPV ORFs ac2 (bro), ac3 (ctl), ac97, ac121 and ac140 from the RoMNPV genome. The average amino acid sequence identity between RoMNPV and AcMNPV ORFs is 96?1 % and there are differences in promoter motif composition for 23 of these ORFs. Maximum-likelihood analysis of selection pressures on AcMNPV and RoMNPV ORFs indicate that ORFs ro18/ac20-ac21 (arif-1) and ro135/ac143 (odv-e18) have undergone positive selection.

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“…However, the images obtained by these authors do not differ from that obtained with the use of the technique three, described in this work, where the faces of the polyhedron appeared smooth, facilitating the morphologic descriptions. Similar methodology is described by Cheng et al (1998), Cheng & Carner (2000 and Cheng et al (2005), used to determine the morphology of SNPV and MNPV of Thysanoplusia orichalcea (L.) (Lepidoptera: Noctuidae).…”

Section: Resultsmentioning

confidence: 99%

Nucleopolyhedrovirus: scanning electron microscopy technique

Torquato¹,

Neto²,

Brancalhão³

et al. 2006

Neotrop. Entomol.

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Neotropical Entomology 35(6):787-790 (2006) Nucleopolyhedrovirus: Técnica de Microscopia Eletrônica de Varredura RESUMO -Um método simples foi desenvolvido para se estudar a forma geométrica do Bombyx mori Nucleopolyhedrovirus múltiplo, BmMNPV, para microscopia eletrônica de varredura. O vírus, pertencente à Família Baculoviridae, foi obtido do fi ltrado, do inóculo e da hemolinfa de lagartas de Bombyx mori (L.) (Lepidoptera: Bombycidae) infectadas. O material foi colocado diretamente sobre o suporte de varredura, onde previamente foi aderida uma fi ta de cobre. Após secagem e metalização com carbono e ouro, o vírus foi analisado ao microscópio de varredura. Os poliedros apresentaramse com as faces lisas, perfeitamente defi nidas, possibilitando a análise matemática que o identifi cou como um octaedro truncado. A forma do poliedro pode apresentar valor taxonômico, uma vez que ela é específi ca para cada linhagem viral. PALAVRAS-CHAVE: Baculovírus, Bombyx mori, BmMNPV, poliedro, octaedro truncado ABSTRACT -A simplifi ed methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the fi ltrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defi ned morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that identifi ed it as a truncated octahedron. The form of the polyhedron can present taxonomic value, once it is specifi c for each viral lineage.KEY WORDS: Baculovirus, Bombyx mori, BmMNPV, polyhedra, truncated octahedron Nucleopolyhedrovirus (NPV) is an important entomopathogenic virus belonging to Baculoviridae family. It is made up of double stranded DNA molecule, associated with proteins of the capsid, forming the nucleocapsid. Two different subgroups are recognized for NPV: the occlusion derived virions and the budded virus. These virion subgroups have different functions in the infection cycle. The occluded subgroups could be divided into two subgroups: those containing one nucleocapsid per virion are referred to as single-capsid NPVs (SNPVs), whereas those containing multiple nucleocapsids per virion are known as multiple NPVs (MNPVs) (Murphy et al. 1995, Blissard 1996.Nucleocapsid and envelope constitutes the virion or infectious particle. In NPV several virions are contained in a protein structure, named occlusion body or polyhedron (Adams & McClintock 1991, Murphy et al. 1995, Blissard 1996. Polyhedra are stable structures that can persist for long time in the environment, where they are commonly found on the surface of the plants or in the soil. In the so...

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Polyhedrin sequence determines the tetrahedral shape of occlusion bodies in Thysanoplusia orichalcea single-nucleocapsid nucleopolyhedrovirus.

Cited by 20 publications

References 29 publications

The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses

The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses

Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses

Nucleopolyhedrovirus: scanning electron microscopy technique

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