Purification and characterization of NAD-dependent formate dehydrogenase from the white-rot fungus Ceriporiopsis subvermispora and a possible role of the enzyme in oxalate metabolism

Watanabe, Tomoki; Hattori, Takefumi; Sabrina, T.; Shimada, Morimi

doi:10.1016/j.enzmictec.2005.01.032

Cited by 37 publications

(33 citation statements)

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“…To our knowledge, C. subvermispora is the first fungus from which both ODC and OXO activities have been measured (Aguilar et al, 1999;Watanabe et al, 2005), and a scheme for the participation of both enzymes in the fungal metabolic reactions and lignin degradation has been proposed (Watanabe et al, 2005). Furthermore in C. subvermispora, ODC is suggested to act sequentially with formate dehydrogenase (FDH).…”

Section: Discussionmentioning

confidence: 99%

Oxalate decarboxylase of the white-rot fungus Dichomitus squalens demonstrates a novel enzyme primary structure and non-induced expression on wood and in liquid cultures

et al. 2009

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Oxalate decarboxylase (ODC) catalyses the conversion of oxalic acid to formic acid and CO 2 in bacteria and fungi. In wood-decaying fungi the enzyme has been linked to the regulation of intraand extracellular quantities of oxalic acid, which is one of the key components in biological decomposition of wood. ODC enzymes are biotechnologically interesting for their potential in diagnostics, agriculture and environmental applications, e.g. removal of oxalic acid from industrial wastewaters. We identified a novel ODC in mycelial extracts of two wild-type isolates of Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene expression when the fungus was cultivated on wood and in liquid medium. However, addition of oxalic acid in liquid cultures caused no increase in transcript amounts, thereby indicating a constitutive rather than inducible expression of Ds-odc. The detected stimulation of ODC activity by oxalic acid is more likely due to enzyme activation than to transcriptional upregulation of the Ds-odc gene. Our results support involvement of ODC in primary rather than secondary metabolism in fungi.

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Section: Discussionmentioning

confidence: 99%

Oxalate decarboxylase of the white-rot fungus Dichomitus squalens demonstrates a novel enzyme primary structure and non-induced expression on wood and in liquid cultures

et al. 2009

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“…This oxalate secretion is mainly due to continuous production of oxalate in primary carbon metabolism. Furthermore, the acid secretion could be due to the absence of oxalate degradation activity in F. palustris, in contrast to oxalate-decomposing white rot fungi (9,39,47,49) and several brown rot fungi such as P. placenta (25) and G. trabeum (10). Moreover, we have suggested that F. palustris transports oxalate out of the cells to prevent possible inhibition of intracellular metabolic reactions.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in the same fungi, a possible role of oxalate degradation in relation to energy metabolism should be evaluated to clarify the possible role of oxalate metabolism in brown rot fungi. In this context, a possible ATP generation by oxalate degradation is hypothesized for P. placenta (20) based on gene expression (24) as proposed for the oxalate-degrading white rot fungus C. subvermispora in which oxalate degradation by oxalate decarboxylase with formate dehydrogenase could produce NADH to be utilized for ATP synthesis (47).…”

Section: Discussionmentioning

confidence: 99%

Oxalate Efflux Transporter from the Brown Rot Fungus Fomitopsis palustris

Watanabe

Shitan

Suzuki

et al. 2010

Appl Environ Microbiol

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An oxalate-fermenting brown rot fungus, Fomitopsis palustris, secretes large amounts of oxalic acid during wood decay. Secretion of oxalic acid is indispensable for the degradation of wood cell walls, but almost nothing is known about the transport mechanism by which oxalic acid is secreted from F. palustris hyphal cells. We characterized the mechanism for oxalate transport using membrane vesicles of F. palustris. Oxalate transport in F. palustris was ATP dependent and was strongly inhibited by several inhibitors, such as valinomycin and NH 4 ؉ , suggesting the presence of a secondary oxalate transporter in this fungus. We then isolated a cDNA, FpOAR (Fomitopsis palustris oxalic acid resistance), from F. palustris by functional screening of yeast transformants with cDNAs grown on oxalic acid-containing plates. FpOAR is predicted to be a membrane protein that possesses six transmembrane domains but shows no similarity with known oxalate transporters. The yeast transformant possessing FpOAR (FpOAR-transformant) acquired resistance to oxalic acid and contained less oxalate than the control transformant. Biochemical analyses using membrane vesicles of the FpOAR-transformant showed that the oxalate transport property of FpOAR was consistent with that observed in membrane vesicles of F. palustris. The quantity of FpOAR transcripts was correlated with increasing oxalic acid accumulation in the culture medium and was induced when exogenous oxalate was added to the medium. These results strongly suggest that FpOAR plays an important role in wood decay by acting as a secondary transporter responsible for secretion of oxalate by F. palustris.

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“…OA in culture medium of F. palustris and in yeast cells was quantified by a commercial kit (Roche, Germany) [10] and GC-MS analysis [15], respectively. Yeast (OD 600 0.1) was cultured at 30°C in 50 ml of SD(-Ura) liquid medium containing 2 mM OA until OD 600 1.0-2.0.…”

Section: Quantification Of Oxalic Acidmentioning

confidence: 99%

“…Another wood-rot white-rot fungus is known to have an OA-resistance system [9][10][11], whereas the system has not been studied in F. palustris.…”

Section: Introductionmentioning

confidence: 99%

Involvement of FpTRP26, a thioredoxin‐related protein, in oxalic acid‐resistance of the brown‐rot fungus Fomitopsis palustris

et al. 2007

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Brown-rot fungus Fomitopsis palustris grows vigorously at high concentrations of oxalic acid (OA), which is fungal metabolite during wood decay. We isolated a cDNA FpTRP26 from F. palustris by functional screening of yeast transformants with cDNAs grown on plates containing OA. FpTRP26 conferred a specific resistance to OA on the transformant. OA-content in transformants grown with 2 mM OA decreased by 65% compared to that of the control. The amount of FpTRP26 transcript in F. palustris amplified with increasing OA-accumulation, and was maintained at high levels even in the stationary phase. Its transcription in F. palustris was inducible in response to exogenously added OA. These results suggest that FpTRP26 is involved in the OA-resistance in F. palustris.

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Purification and characterization of NAD-dependent formate dehydrogenase from the white-rot fungus Ceriporiopsis subvermispora and a possible role of the enzyme in oxalate metabolism

Cited by 37 publications

References 38 publications

Oxalate decarboxylase of the white-rot fungus Dichomitus squalens demonstrates a novel enzyme primary structure and non-induced expression on wood and in liquid cultures

Oxalate decarboxylase of the white-rot fungus Dichomitus squalens demonstrates a novel enzyme primary structure and non-induced expression on wood and in liquid cultures

Oxalate Efflux Transporter from the Brown Rot Fungus Fomitopsis palustris

Involvement of FpTRP26, a thioredoxin‐related protein, in oxalic acid‐resistance of the brown‐rot fungus Fomitopsis palustris

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