Involvement of FpTRP26, a thioredoxin‐related protein, in oxalic acid‐resistance of the brown‐rot fungus <i>Fomitopsis palustris</i>

Watanabe, Tomoki; Shitan, Nobukazu; Umezawa, Toshiaki; Yazaki, Kazufumi; Shimada, Morimi; Hattori, Takefumi

doi:10.1016/j.febslet.2007.03.070

Cited by 1 publication

(8 citation statements)

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“…palustris culture conditions. Two mycelial plugs (5 mm in diameter) of F. palustris (Berkely et Curtis) Murill TYP6137 were grown as a stationary culture in a 200-ml Erlenmeyer flask containing 40 ml of liquid medium as previously reported (48).…”

Section: Methodsmentioning

confidence: 99%

“…Functional screening of a Saccharomyces cerevisiae AD12345678 (6) transformant with an F. palustris cDNA library with vector pDR196 (50) was carried out to screen the transformants to grow with 12 mM oxalic acid (48). Because the cDNA fragment recovered from oxalic acid-resistant yeast showing a strong phenotype lacked the 5Ј region, the 5Ј end of the cDNA was determined with the Gene Racer kit (Invitrogen) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Accordingly, these cDNAs were expected to encode transporters catalyzing oxalate transport out of the cells or oxalate-decomposing enzymes and other proteins possessing unidentified functions. By this strategy, we successfully isolated the cDNA FpTRP26 conferring oxalic acid resistance for F. palustris (48). We have further characterized the remaining transformants showing oxalic acid resistance (48) and obtained the gene product, FpOAR (Fomitopsis palustris oxalic acid resistance), which is a putative plasma membrane protein that showed distinct oxalate transport activity in yeast membrane vesicles.…”

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confidence: 99%

“…By this strategy, we successfully isolated the cDNA FpTRP26 conferring oxalic acid resistance for F. palustris (48). We have further characterized the remaining transformants showing oxalic acid resistance (48) and obtained the gene product, FpOAR (Fomitopsis palustris oxalic acid resistance), which is a putative plasma membrane protein that showed distinct oxalate transport activity in yeast membrane vesicles. The oxalate transport property of FpOAR was similar to that of F. palustris membrane vesicles.…”

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confidence: 99%

“…To isolate a cDNA encoding the oxalate transporter, we previously isolated fungal cDNAs from yeast transformants with cDNA of F. palustris grown in the presence of a high oxalate concentration that is lethal to wild-type cells (48). Even if oxalate was incorporated into the cells, the transformants probably transported oxalate out of the cells or it was degraded by the gene products of the cDNA from F. palustris.…”

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Oxalate Efflux Transporter from the Brown Rot Fungus Fomitopsis palustris

Watanabe

Shitan

Suzuki

et al. 2010

Appl Environ Microbiol

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An oxalate-fermenting brown rot fungus, Fomitopsis palustris, secretes large amounts of oxalic acid during wood decay. Secretion of oxalic acid is indispensable for the degradation of wood cell walls, but almost nothing is known about the transport mechanism by which oxalic acid is secreted from F. palustris hyphal cells. We characterized the mechanism for oxalate transport using membrane vesicles of F. palustris. Oxalate transport in F. palustris was ATP dependent and was strongly inhibited by several inhibitors, such as valinomycin and NH 4 ؉ , suggesting the presence of a secondary oxalate transporter in this fungus. We then isolated a cDNA, FpOAR (Fomitopsis palustris oxalic acid resistance), from F. palustris by functional screening of yeast transformants with cDNAs grown on oxalic acid-containing plates. FpOAR is predicted to be a membrane protein that possesses six transmembrane domains but shows no similarity with known oxalate transporters. The yeast transformant possessing FpOAR (FpOAR-transformant) acquired resistance to oxalic acid and contained less oxalate than the control transformant. Biochemical analyses using membrane vesicles of the FpOAR-transformant showed that the oxalate transport property of FpOAR was consistent with that observed in membrane vesicles of F. palustris. The quantity of FpOAR transcripts was correlated with increasing oxalic acid accumulation in the culture medium and was induced when exogenous oxalate was added to the medium. These results strongly suggest that FpOAR plays an important role in wood decay by acting as a secondary transporter responsible for secretion of oxalate by F. palustris.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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