Purification and Biochemical Characterization of Cell Wall-bound Trehalase from Pericarp Tissues of Actinidia deliciosa

Li, Xingjun; Nakagawa, Naoki; Sakurai, Naoki

doi:10.2503/jjshs1.77.229

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…An increased amount of trehalose, nonreducing disaccharide, in fruits has been reported to improve shelf life [41]. In this study, the gene that encodes trehalase (α-glucoside-1-glucohydrolase), a hydrolytic cell wall-bound enzyme that cleaves trehalose into two glucose moieties [41] was upregulated due to ethylene treatment. This phenomenon degrades apoplastic trehalose which in turn causes softening of the fruit ( Figure 5).…”

Section: Firmness and Genes Related To Softeningmentioning

confidence: 72%

“…In addition, the genes that encode aquaporin PIP1-4-like and aquaporin PIP2-7-like were downregulated probably due to the reduction of the transport of water and solutes across the cell membrane [46] as the fruit ripened. 7 wall-bound enzyme that cleaves trehalose into two glucose moieties [41] was upregulated due to ethylene treatment. This phenomenon degrades apoplastic trehalose which in turn causes softening of the fruit ( Figure 5).…”

Section: Firmness and Genes Related To Softeningmentioning

confidence: 99%

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Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

et al. 2020

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Fruit ripening involves changes in physical, physiological and metabolic activities through the actions of enzymes and regulatory genes. This study was initiated to identify the genes related to the ripening of kiwifruit. Gold ‘Haegeum’ kiwifruit is a yellow-fleshed kiwifruit cultivar usually used for fresh marketing. The fruit is harvested at a physiologically mature but unripe stage for proper storage, marketing distribution and longer shelf life. To identify the differentially expressed genes (DEGs) during ripening, fruit treated with ethylene were compared with control fruit that ripened naturally without ethylene treatment. Firmness, respiration rate, ethylene production rate, total soluble solids (TSS), titratable acidity (TA), brix acid ratio (BAR) and overall acceptability were taken during the study as fruit ripening indicators. Total mRNAs were sequenced by Illumina high-throughput sequencing platform and the transcriptome gene set was constructed by de novo assembly. We identified 99,601 unigenes with an average length of 511.77 bp in transcriptome contigs. A total of 28,582 differentially expressed unigenes were identified in the ethylene treatment vs. control. Of these 28,582 unigenes, 13,361 and 15,221 genes were up- and downregulated, respectively, in the treated fruit. The results also showed that 1682 and 855 genes were up- and downregulated, respectively, more than 2-fold at p < 0.05 in fruit treated with ethylene as compared with the control fruit. Moreover, we identified 75 genes showing significantly different expression; 42 were upregulated, and 33 were downregulated. A possible category of the identified ripening-related genes was also made. The findings of this study will add to the available information on the effect of ethylene treatment on ripening and the related changes of kiwifruit at the genomic level, and it could assist the further study of genes related to ripening for kiwifruit breeding and improvement.

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Section: Firmness and Genes Related To Softeningmentioning

confidence: 72%

Section: Firmness and Genes Related To Softeningmentioning

confidence: 99%

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

et al. 2020

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“…In plants, trehalase participates in the regulation of sucrose and starch, confers stress resistance [42], and participates in the formation and development of bean nodules. Trehalase has been found in chickpea (Cicer arietinum L.) seeds [43] and kiwi fruit (Actinidia deliciosa) [44].…”

Section: Animals and Plantsmentioning

confidence: 99%

“…Studies on the substrate specificity of most trehalases show that they are highly specific towards trehalose and have no catalytic effect on other disaccharides such as brown sugar, maltose, isomaltose, furanose, maltose, palatinose, and sucrose. The trehalase purified from the cell walls of Actinidia deliciosa fruit displayed weak activity with maltose and maltotriose [44]. Trehalase from Candida catalyzes on both trehalose and sucrose breakdown, and the ratio between trehalase and invertase activity is 1:3.5 [91].…”

Section: Substrate Specificitymentioning

confidence: 99%

Characterization, heterologous expression and engineering of trehalase for biotechnological applications

Gao

Gong

et al. 2022

Syst Microbiol and Biomanuf

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Trehalose is a non-reducing disaccharide connected by α-1,1-glycosidic bonds; it is widely distributed in bacteria, fungi, yeast, insects, and plant tissues and plays various roles. It can be hydrolyzed by trehalase into two glucose molecules. Trehalases from different sources have been expressed in Escherichia coli, Pichia pastoris, Saccharomyces cerevisiae, baculovirus-silkworm, and other expression systems; however, it is most common in E. coli. The structural characteristics of different glycoside hydrolase (GH) family trehalases and the sources of trehalase have been analyzed. The catalytic mechanism of GH37 trehalase has also been elucidated in detail. Moreover, the molecular modification of trehalase has mainly focused on directed evolution for improving enzyme activity. We comprehensively reviewed the current application status and adaptable transformations was comprehensively overviewed in the context of industrial performance. We suggest that the level of recombinant production is far from meeting industrial requirements, and the catalytic performance of trehalase needs to be improved urgently. Finally, we discuss developmental prospects and future trends.

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Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method

Suehara

Kameoka

Hashimoto

2012

Bioprocess Biosyst Eng

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The kinetic behavior of the sugar uptake phenomena of a suspension of Arabidopsis cells was investigated by mid-infrared spectroscopy using Fourier transform infrared spectrometers and attenuated total reflection techniques. The kinetic behavior of the cell growth was also studied and the growth and the sugar uptake behaviors were discussed for three typical plant cells (Arabidopsis, TBY-2, and rice cells). The cell growth rate and the lag period were influenced by not only the types of the plant cells, but also the sugar species used as the carbon source. The characteristics of the sugar uptake behavior were clarified based on the difference in the three types of plant cells. The cell growth and the sugar uptake progressed at approximately the same time in the TBY-2 cells. In the rice cells, the sugar uptake rate was relatively lower than that of the others. On the other hand, the sugar uptake of the Arabidopsis cells started before the cell growth. Furthermore, glucose as the carbon source of the Arabidopsis cell cultivation seems to significantly influence the sugar metabolism. Glucose had a significant influence on the sugar metabolism of the other sugar under the conditions for the mixture of glucose and the other sugar. The characteristics of the sugar uptake phenomena based on the cell growth stage was typical for each plant cell except for some sugars, such as galactose and trehalose, and the behavior of the total sugar uptake had not changed. These results suggested that the cell growth and the sugar uptake in the plant cell cultivation processes may be controlled by the combined supply of the sugar species as the carbon source. The detailed data for plant cell cultivation using each sugar obtained in this study would be useful for bioscience research and for cultivation process control using various sugars, for example, purified or sugar mixtures formed from biomass materials.

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Purification and Biochemical Characterization of Cell Wall-bound Trehalase from Pericarp Tissues of Actinidia deliciosa

Cited by 5 publications

References 16 publications

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

Characterization, heterologous expression and engineering of trehalase for biotechnological applications

Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method

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