Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method

Abstract

The kinetic behavior of the sugar uptake phenomena of a suspension of Arabidopsis cells was investigated by mid-infrared spectroscopy using Fourier transform infrared spectrometers and attenuated total reflection techniques. The kinetic behavior of the cell growth was also studied and the growth and the sugar uptake behaviors were discussed for three typical plant cells (Arabidopsis, TBY-2, and rice cells). The cell growth rate and the lag period were influenced by not only the types of the plant cells, but also the sugar species used as the carbon source. The characteristics of the sugar uptake behavior were clarified based on the difference in the three types of plant cells. The cell growth and the sugar uptake progressed at approximately the same time in the TBY-2 cells. In the rice cells, the sugar uptake rate was relatively lower than that of the others. On the other hand, the sugar uptake of the Arabidopsis cells started before the cell growth. Furthermore, glucose as the carbon source of the Arabidopsis cell cultivation seems to significantly influence the sugar metabolism. Glucose had a significant influence on the sugar metabolism of the other sugar under the conditions for the mixture of glucose and the other sugar. The characteristics of the sugar uptake phenomena based on the cell growth stage was typical for each plant cell except for some sugars, such as galactose and trehalose, and the behavior of the total sugar uptake had not changed. These results suggested that the cell growth and the sugar uptake in the plant cell cultivation processes may be controlled by the combined supply of the sugar species as the carbon source. The detailed data for plant cell cultivation using each sugar obtained in this study would be useful for bioscience research and for cultivation process control using various sugars, for example, purified or sugar mixtures formed from biomass materials.

FOURIER TRANSFORM INFRARED CHARACTERIZATION OF SALICYLIC ACID TREATED RICE PLANTS AGAINST Xanthomonas oryzae pv. oryzae

“…The defensive activity at the below leaves is more abundant than that of the above leaves. [16,19] Amide I 1700-1600 Amide I due to C=O stretching of α-helix protein [16,18] C-O Stretching lignin and hemicellulose 1300-1200 C-C, C-O skeletal [17,20] C-O-C glycoside 1103 C-O-C glycoside ether mainly hemicellulose [19] C-C , C-O Stretching 1022, 1047, 1080 Mainly C-O-C of polysaccharides [20,21] Two-dimensional PCA analyses in above and below rice leaves were presented on Figure 3. The blue points representing control treatment, scattered widely on the right side of the graph area, could easily be discriminated from the red points of the SA-treated one, with both above and below rice leaves ( Figure 3A and 3B).…”

Advances in process monitoring tools for cell culture bioprocesses

“…There have been many reports on the use of NIR or middle‐IR (MIR), especially using ATR probes, for the measurement of glucose and other medium components in microbial and plant cell processes . Using an instrumentation system that employs a proprietary signal processing algorithm, it was shown that NIR can be used to monitor glycerol, methanol, and biomass concentration in the fermentation of Pichia pastoris .…”

Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis

“…However, it is important that the food models are prepared by including the living cells because most of the flesh food will include a living cell in the body, or the exogenous cells will invade the food. We also studied the quantitative analysis and kinetic analysis of sugar metabolism for plant cells in the basal MS salts media [19] using the FT-IR/ATR method [20–27]. The MIR spectrum has shown that the spectral additivity was experimentally applicable for sucrose, glucose, and fructose in the MS media.…”