Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells

Abstract

IntroductionPure paclitaxel nanoparticles (PPN), consisting entirely of drug molecules, were prepared by the electrostatic spraying method as promising candidates for antitumor application. Compared with the traditional preparation method, the advantage of the electrostatic spraying method included high production rates, relatively small particle sizes, and ease of preparation.Materials and methodsPaclitaxel was used to prepared PPN by electrostatic spray. The electrostatic spray device included a constant speed pump with a syringe, a high-voltage power supply, and a metal foil receiver was used to prepare and evaluate PPN. The syringe drew off a certain amount of paclitaxel chloroform solution (150 μg/mL) and was placed on the constant speed injection pump. The dissolution behavior of PPN was evaluated by dissolution test and the presence of paclitaxel in PPN was detected by X-Ray powder diffraction and differential scanning calorimetry. Effect of PPN on SMMC-7721 cells were studied by cell uptake, cell apoptosis and antitumor study.ResultsThe results of X-ray powder diffraction and differential scanning calorimetry characterization showed that the PPN were in an amorphous state. A dissolution study indicated that PPN have a significantly enhanced dissolution rate of paclitaxel. Moreover, SMMC-7721 tumor cells treated with PPN exhibited a distinctly high uptake rate that promoted cell apoptosis. An in vivo antitumor study demonstrated that PPN had significant antitumor efficacy.ConclusionAll conclusions verified that electrostatic spraying is a potential technology for developing PPN, and PPN can be regarded as a promising treatment for cancer.

Development and Evaluation of Paclitaxel and Curcumin Dry Powder for Inhalation Lung Cancer Treatment

“…The results of dissolution studies of CUR alone, PTX alone and the combination of CUR/PTX at varying ratios under controlled conditions are illustrated in Figure 2 . The dissolutions of CUR alone and PTX alone exhibited similar patterns, whereby approximately 50% of the drugs were dissolved after 12 h. Our data accord with other published findings under similar controlled conditions [ 16 , 17 ]. In addition, the dissolutions of combination formulations also followed similar trend.…”

Preparation of paclitaxel-folic acid functionalized gelatin grafted mesoporous hollow carbon nanospheres for enhancing antitumor effects toward liver cancer (SMMC-7721) cell lines

“…The supernatant obtained by centrifugation (20 µl) was used for the analysis of the PTX content by HPLC. 31…”

Folate-functionalized SMMC-7721 liver cancer cell membrane-cloaked paclitaxel nanocrystals for targeted chemotherapy of hepatoma

“…After staining with Coomassie Brilliant Blue, image development was performed under a microscope and analyzed by Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA). In addition, western blotting was used to analyze SMMC-7721 CM proteins, including CD147 (Zhu et al., 2015 ), EGFR (Psyrri et al., 2012 ; Zhao et al., 2017 ), EpCAM, N-cadherin, and Galectin-3 (Chen et al., 2016 ; Liu et al., 2019 ), to further verify the coating process.…”