Preparation of paclitaxel-folic acid functionalized gelatin grafted mesoporous hollow carbon nanospheres for enhancing antitumor effects toward liver cancer (SMMC-7721) cell lines

Abstract

Folic acid functionalized gelatin-coated mesoporous hollow carbon nanospheres (FGMCN) were synthesized and applied to enhance the antitumor curative effect of paclitaxel (PTX) for human liver cancer cell lines (SMMC-7721). PTX was loaded in FGMCN by the adsorption method and the PTX-loaded samples (PTX-FGMCN) had a drug content of 29.8 ± 1.06%. The PTX-FGMCN with a sustained release effect was characterized by X-ray diffraction and differential scanning calorimeter in order to analyze the PTX state in FGMCN. In vitro cell experiments showed that FMHSN improves the uptake of PTX and promotes apoptosis due to the nano-targeting effect of FMHSN. An in vivo tumor bearing experiment in mice indicated that the PTX-FGMCN significantly inhibited the growth of tumors. All of these results suggested that the PTX-FGMCN may be an effective anti-hepatoma drug in the future.

Folate-functionalized SMMC-7721 liver cancer cell membrane-cloaked paclitaxel nanocrystals for targeted chemotherapy of hepatoma

“…The rapid development of nanotechnology provides new ideas for the treatment and diagnosis of cancer (Manzur et al., 2017 ; He et al., 2018 ; Dong et al., 2019 ). Currently, various nanocarriers, such as mesopore silica (He et al., 2017 ), liposomes (Eloy et al., 2020 ), carbon nanotubes (Soleyman et al., 2015 ), polymer nanoparticles (Levit et al., 2020 ), and cyclodextrin nanoparticles (Yan et al., 2019 ), have been widely incorporated with PTX to improve its solubility and targeting ability. However, these methods generally have problems including low drug loading and targeting ability.…”

Preparation and anti-cancer activity of transferrin/folic acid double-targeted graphene oxide drug delivery system

“…19,20 Recent articles reported that FA receptors and Tf receptors were highly expressed on the surface of SMMC-7721 cells (a typical hepatoma cell line), and Tf-targeted drug carriers could deliver the drugs to tumor sites. 21,22 However, most of the current researches concerning targeted drug delivery systems are focused only on a single target, and there are still problems of premature or accelerated drug release and difficulty in controlling time of release that, in turn, may reduce drug concentration below optimal levels at the tumor site. These problems result in undesirable drug resistance.…”

“…19,20 Recent articles reported that FA receptors and Tf receptors were highly expressed on the surface of SMMC-7721 cells (a typical hepatoma cell line), and Tf-targeted drug carriers could deliver the drugs to tumor sites. 21,22…”