Folate-functionalized SMMC-7721 liver cancer cell membrane-cloaked paclitaxel nanocrystals for targeted chemotherapy of hepatoma

Abstract

In this study, we prepared a folic acid-functionalized SMMC-7721 liver cancer cell membrane (CM)-encapsulated paclitaxel nanocrystals system (FCPN) for hepatoma treatment. Transmission electron microscopy (TEM) characterization showed that FCPN was irregular spherical shapes with a particle size larger than 200 nm and a coated thickness of approximately 20 nm. In an in vitro release experiment, FCPN indicated a slowly release effect of paclitaxel (PTX). Cell experiments demonstrated that FCPN was taken up by SMMC-7721 cells and significantly inhibited the proliferation of SMMC-7721 cells, which illustrated that FCPN had good targeting ability compared with PN and CPN. According to the results of in vivo animal experiments, FCPN significantly inhibited tumor growth. Tissue distribution experiments proved that FCPN could accumulate significantly in tumor tissues, which further explained why FCPN had good targeting ability. These results clearly suggested that folate-functionalized homotypic CM bionic nanosystems might represent a very valuable method for liver cancer treatment in the future.

Advances in cell membrane-based biomimetic nanodelivery systems for natural products

“…For example, SMMC-7721 cell membranes were modified with folic acid and used to coat PTX-loaded nanocrystals. The functionalized nanoplatform released PTX in a sustained manner and showed better targeting effects and biocompatibility than unmodified nanocrystals (Shen et al., 2022 ). In another study, the LWSW quorum sensing peptide was used to prepare a nanoplatform that selectively crossed the BBB and targeted glioma and neovascular endothelial cells (Ran et al., 2017 ).…”

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

“…Similarly, as a commonly used chemotherapeutic drug, paclitaxel also faces many limiting problems in clinical cancer treatment. Its bioavailability and antitumor activity in the antitumor process have also been shown to be enhanced by encapsulation with liver cancer cell membranes [ 75 ].…”

Cancer Cell Membrane‐Based Materials for Biomedical Applications

“…These molecules can be easily coupled onto the surfaces of the polymeric biomaterials. [77] Polymeric Nanomaterials: PLGA, [78][79][80] PEG-PLGA, [81,82] PLA, [83] PDA, [84] liquid crystalline lipid nanoparticles, [85] liposome, [86,87] nanogel, [88,89] nanocrystals, [90,91] nanoemulsion, [92] nanosuspensions, [93] and poloxamer 407(F127) nanoparticles. [58] It is noteworthy that in the event that the NPs possess a negative charge, an electrostatic repulsion occurs between them and the negatively charged extracellular membrane.…”