Pulsed electric field application reduces carbapenem- and colistin-resistant microbiota and blaKPC spread in urban wastewater

Ballash, Gregory A.; Lee, Seungjun; Mollenkopf, Dixie F.; Mathys, Dimitria A.; Albers, Amy L.; Sechrist, Emily; Feicht, Sydnee M.; Rubio, Joanny C. Van Balen; Sullivan, Sean; Lee, Ji-Young; Wittum, Thomas E.

doi:10.1016/j.jenvman.2020.110529

Cited by 11 publications

(8 citation statements)

References 45 publications

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“…The dissemination of antibiotic resistance genes (ARGs) worldwide, especially the carbapenemase family (CRG), became an urgent global health threat [ 9 , 10 ], mostly because their presence was detected in both clinical and environmental settings [ 11 ]. Wastewaters represent a hotspot for the development and dissemination of AMR and ARGs [ 12 ], being a key factor in this process [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

et al. 2021

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Carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated from influent (I) and effluent (E) of two wastewater treatment plants, with (S1) or without (S2) hospital contribution, were investigated. The strains belonged to the Kp1 phylogroup, their highest frequency being observed in S1, followed by S2. The phenotypic and genotypic hypervirulence tests were negative for all the strains tested. At least one carbapenemase gene (CRG), belonging to the blaKPC, blaOXA-48, blaNDM and blaVIM families, was observed in 63% of CPKP, and more than half co-harboured two to four CRGs, in different combinations. Only five CRG variants were observed, regardless of wastewater type: blaKPC-2, blaNDM-1, blaNDM-6, blaVIM-2, and blaOXA-48. Sequence types ST258, ST101 and ST744 were common for both S1 and S2, while ST147, ST525 and ST2502 were found only in S1 and ST418 only in S2. The strains tested were multi-drug resistant (MDR), all being resistant to beta-lactams, cephalosporins, carbapenems, monobactams and fluoroquinolones, followed by various resistance profiles to aminoglycosides, trimethoprim-sulphamethoxazole, tigecycline, chloramphenicol and tetracycline. After principal component analysis, the isolates in S1 and S2 groups did not cluster independently, confirming that the antibiotic susceptibility patterns and gene-type profiles were both similar in the K. pneumoniae investigated, regardless of hospital contribution to the wastewater type.

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Section: Introductionmentioning

confidence: 99%

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

et al. 2021

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“…One reasonable source of ARB environmental contamination is surface water that receives treated wastewater effluent. Despite treatment, wastewater effluent can carry large amounts of ARB, some of which include resistance to drugs of last resort like carbapenems and colistin (Rizzo et al, 2013;Mathys et al 2019;Ballash et al 2020). In large metropolitan areas, effluent can be a combination of wastewater from wastewater treatment plants, septic systems, and combine sewer overflows (CSO), which allows untreated wastewater to discharge directly into surface water.…”

Section: Resultsmentioning

confidence: 99%

Temporal Trends in Antimicrobial Resistance of Fecal Escherichia coli from Deer

et al. 2021

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The changing epidemiologic role of wildlife as reservoirs of antimicrobial-resistant bacteria (ARB) is poorly understood. In this study, we characterize the phenotypic resistance of commensal Escherichia coli from fecal samples of 879 individual white-tailed (Odocoileus virginianus; WTD) over a ten-year period and analyze resistance patterns. Our results show commensal E. coli from WTD had significant linear increases in reduced susceptibility to 5 of 12 antimicrobials, including broad-spectrum cephalosporins and fluoroquinolones, from 2006 to 2016. In addition, the relative frequency distribution of minimal inhibitory concentrations of two additional antimicrobials shifted towards higher values from across the study period. The prevalence of multidrug-resistant commensal E. coli increased over the study period with a prevalence of 0%, 2.2%, and 3.7% in 2006, 2012, and 2016, respectively. WTD may be persistently and increasingly exposed to antibiotics or their residues, ARB, and/or antimicrobial resistance genes via contaminated environments like surface water receiving treated wastewater effluent.

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“…Since PEF is a non-chemical disinfection method, it avoids the emission of residual disinfectants and their by-products 82), 83) . Ballash et al (2020) 84) reported the reduction effect of PEF on carbapenem-or colistin-resistant E. coli and Enterobacteriaceae, as well as blaKPC. The reduction ratios of each ARB and ARGs by low (30)(31)(32)(33)(34)(35) kV/cm for 50-125 µsec.)…”

Section: Emerging Disinfection Technologiesmentioning

confidence: 99%

Summary and Perspectives on Current Disinfection Technologies in Reducing Antibiotic Resistant Bacteria and Their Resistance Genes

Furukawa

Amarasiri

Ueno

et al. 2022

Japanese J. Wat. Treat. Biol.

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Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are an emerging contaminant of concern. However, the present sewage treatment plants (STPs) are not designed with treatment units for direct removal of ARB and ARGs. STPs are considered to be hotspots of horizontal gene transfer of ARGs as well as a major source of their emissions in the aquatic environments. In order to mitigate the risk of infection by ARB and ARGs, their reduction in STPs needs to be fully understood. This review focused on disinfection, the tertiary treatment of the sewage treatment process, and discussed the advantages, limitations, and improvements in the process using each disinfection technology, with the aim of identifying treatment strategies and further research directions for future ARB and ARGs in reducing emissions into the environment.

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Pulsed electric field application reduces carbapenem- and colistin-resistant microbiota and blaKPC spread in urban wastewater

Cited by 11 publications

References 45 publications

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

Temporal Trends in Antimicrobial Resistance of Fecal Escherichia coli from Deer

Summary and Perspectives on Current Disinfection Technologies in Reducing Antibiotic Resistant Bacteria and Their Resistance Genes

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