Pulp regeneration in a full-length human tooth root using a hierarchical nanofibrous microsphere system

Li, Xiangwei; Ma, Chi; Xie, Xiaoyan; Sun, Hongchen; Liu, Xiaohua

doi:10.1016/j.actbio.2016.02.040

Cited by 81 publications

(83 citation statements)

References 33 publications

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“…Dentin was reported to have a bone-inducing function in 1967, and DDM was found to have better regenerative properties than undemineralized dentin matrix (UDM). Since then, several other studies were published on the use of DDM in tissue engineering [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. However, to date, a literature search has revealed no review article on the application of DDM for teeth and bone regenerations.…”

Section: Introductionmentioning

confidence: 99%

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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Demineralized dentin matrix (DDM), derived from human teeth, is an excellent scaffold material with exciting bioactive properties to enhance bone and dental tissue engineering efficacy. In this article, first the nano-structure and bioactive components of the dentin matrix were reviewed. Then the preparation methods of DDM and the resulting properties were discussed. Next, the efficacy of DDM as a bone substitute with in vitro and in vivo properties were analyzed. In addition, the applications of DDM in tooth regeneration with promising results were described, and the drawbacks and future research needs were also discussed. With the extraction of growth factors from DDM and the nano-structural properties of DDM, previous studies also broadened the use of DDM as a bioactive carrier for growth factor delivery. In addition, due to its excellent physical and biological properties, DDM was also investigated for incorporation into other biomaterials design and fabrication, yielding great enhancements in hard tissue regeneration efficacy.

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Section: Introductionmentioning

confidence: 99%

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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“…It is a potential signaling protein that can promote angiogenesis and activate dentin regeneration (2). VEGF has been used in growth factor delivery-based tissue engineering (3), including pulp tissue regeneration for regenerative endodontics (4). Moreover, VEGF has been shown to induce proliferation and differentiation of human pulp cells into odontoblasts (5).…”

Section: Introductionmentioning

confidence: 99%

Effect of recombinant vascular endothelial growth factor and translationally controlled tumor protein on 2‑hydroxyethyl methacrylate‑treated pulp cells

2018

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Vascular endothelial growth factor (VEGF)-A is a potential signaling protein that may promote angiogenesis. VEGF also helps cells survive in stressfull or hazardous conditions. The present study aimed to compare the effect of VEGF with translationally controlled tumor protein (TCTP), an anti‑apoptotic protein in human dental pulp cells (HDPCs), following exposure to 2‑hydroxyethyl methacrylate (HEMA), which is a major residual monomer from resin restorative dental materials. Cell viability, alkaline phosphatase (ALP) activity, mineralization and gene expressions for odontogenic and osteogenic differentiation markers of HDPCs were investigated, following exposure to HEMA and in combination with TCTP and VEGF. The results revealed that TCTP at 1 ng/ml and VEGF at 10 ng/ml significantly promoted the proliferation of HDPCs (P<0.05). TCTP (1 ng/ml) and VEGF (10 ng/ml) maintained the cell viability of 4 mM HEMA‑treated cells at the same percentage as the control. However, cells treated with HEMA+TCTP+VEGF had a lower cell viability than the groups treated with HEMA and TCTP or VEGF alone. TCTP and VEGF promoted cell proliferation, ALP activity and mineralization, and upregulated of DSPP, DMP‑1, BMP‑2, and ALP mRNA expression compared with the control. Furthermore, the HEMA+TCTP and HEMA+VEGF groups had significantly higher percentages of calcium deposition than HEMA‑treated cells (P<0.001). HEMA was cytotoxic to HDPCs, reduced ALP activity and caused the significant downregulation of odontogenic and osteogenic gene expressions (P<0.05). It was concluded that VEGF and TCTP promoted pulp cell growth and the survival of HEMA‑treated cells without synergistic effects. TCTP was required in lower concentrations for these effects. VEGF and TCTP enhanced cell differentiation and mineralization.

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“…The in vivo results showed the regeneration of pulp tissues that fulfilled the entire lower two-thirds and reached the coronal third of the full-length root canal. In addition, a large number of blood vessels were regenerated throughout the canal [187]. Therefore, our work demonstrates the success of pulp tissue regeneration in a full-length root with one end sealed using a single installation process, making it a significant step toward regenerative endodontics.…”

Section: Applications Of Injectable Scaffolds In Dental and Craniomentioning

confidence: 82%

Section: Figmentioning

confidence: 99%

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Chang

Ahuja

et al. 2017

Materials Science and Engineering: R: Reports

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198

170

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Injectable scaffolds are appealing for tissue regeneration because they offer many advantages over pre-formed scaffolds. This article provides a comprehensive review of the injectable scaffolds currently being investigated for dental and craniofacial tissue regeneration. First, we provide an overview of injectable scaffolding materials, including natural, synthetic, and composite biomaterials. Next, we discuss a variety of characteristic parameters and gelation mechanisms of the injectable scaffolds. The advanced injectable scaffolding systems developed in recent years are then illustrated. Furthermore, we summarize the applications of the injectable scaffolds for the regeneration of dental and craniofacial tissues that include pulp, dentin, periodontal ligament, temporomandibular joint, and alveolar bone. Finally, our perspectives on the injectable scaffolds for dental and craniofacial tissue regeneration are offered as signposts for the future advancement of this field.

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Pulp regeneration in a full-length human tooth root using a hierarchical nanofibrous microsphere system

Cited by 81 publications

References 33 publications

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

Effect of recombinant vascular endothelial growth factor and translationally controlled tumor protein on 2‑hydroxyethyl methacrylate‑treated pulp cells

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

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