Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Chang, Bei; Ahuja, Neelam; Ma, Chi; Liu, Xiaohua

doi:10.1016/j.mser.2016.11.001

Cited by 199 publications

(184 citation statements)

References 216 publications

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“…The intelligent character of the copolymers obtained is based on the phase transition LCST that presented around a temperature range T~30-35°C, values close to the body temperature (37.4°C), giving the material potentiality for its use in biomedical applications. In the literature it has been reported that by adding a new component to PNIPAm (covalently bound or by physical mixtures) it is possible to change its LCST transition temperature to higher or lower values 11,12,19 . What has been mentioned is that when some hydrophilic phase is added to the PNIPAm, the LCST is displaced at higher temperatures 5 , while in the presence of compounds with greater hydrophobic character, the opposite effect occurs 2,20 .…”

Section: Study Of the Lcst Of The Synthesized Copolymersmentioning

confidence: 99%

“…In the case of hydrogels sensitive to changes in temperature are characterized by having a lower critical solution temperature (LCST) 9,10 . Among the smart polymers we can mention PNIPAm, which is a non-biodegradable polymer having an LCST of approximately 32-34°C (close to human body temperature 37.4°C) 7,11 . However, being a synthetic polymer, the PNIPAm could present problems of biocompatibility and biodegradability, so it is of great interest, the incorporation of a phase that can improve this condition, especially if it is intended to be used in some biomedical applications 12 .…”

Section: Introductionmentioning

confidence: 99%

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Intelligent copolymers based on poly (N-isopropylacrylamide) PNIPAm with potential use in biomedical applications. Part i: PNIPAm functionalization with 3-butenoic acid and piperazine

Carrero

Posada

Sabino

2018

IJAMB

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The synthesis and characterization of the thermosensitive copolymers based on Poly (N-Isopropylacrylamide) (PNIPAm) and 3-butenoic acid and functionalized with piperazine was carried out. The free radical polymerization of the PNIPA copolymer with 3-butenoic acid was performed under microwave radiation. After obtaining this copolymer, the carboxyl groups present in the copolymer chain were activated with 1-ethyl- (3-3-dimethylaminopropyl) carbodiimide in the presence of N-hydroxysuccinimide, improving its reactivity to incorporate the piperazine through its amino group. The characterization consisted: differential scanning calorimetric and ultraviolet-visible spectrophotometry to determine the LCST phase transition temperature, ranging from (30-35)°C. Structurally it was analyzed by infrared spectroscopy. A morphological analysis was performed using scanning electron microscopy, after simulating an injectable process, with the objective to observe internally the porosity and interconnectivity. The biocompatibility was evaluated through hemocompatibility tests and it was observed that the copolymers obtained were not cytotoxic. In base of the results, the chemical structure of these new copolymers confers a functionality that allows them to serve as nuclei to graft other molecules, such as polysaccharides. Then, the results obtained on the LCST temperature, porosity, interconnected pore network morphology, the ability to be injectable and the biocompatible nature of these copolymers are indicative that these new synthetic biomaterials have the potential to be used in biomedical, pharmacological and for tissue engineering. Also, once their biocompatibility was demonstrate, they may serve to generate interesting compounds having chemical anchor points for the possible addition of polysaccharides using insertion reactions, thereby generating graft copolymers with potential use in biomedical applications.

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Section: Study Of the Lcst Of The Synthesized Copolymersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intelligent copolymers based on poly (N-isopropylacrylamide) PNIPAm with potential use in biomedical applications. Part i: PNIPAm functionalization with 3-butenoic acid and piperazine

Carrero

Posada

Sabino

2018

IJAMB

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“…The injectable hydrogels are paid more attention as they can be injected to the damaged part in situ with easily taking on complex shape and adhering to the surrounding tissues during hydrogels formation . Moreover, the injectable system have some advantages over prefabricated scaffold, including easy application, avoiding operational trauma and improving patient compliance and comfort, which have been paid more attention in tissue engineering scaffolds …”

Section: Introductionmentioning

confidence: 99%

“…19 Moreover, the injectable system have some advantages over prefabricated scaffold, including easy application, avoiding operational trauma and improving patient compliance and comfort, which have been paid more attention in tissue engineering scaffolds. 20,21 Alginate (Alg), a natural copolymer of polysaccharides, is derived from algae. Alg is widely used in food and pharmaceutical industries with favorable degradability and biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Injectable hydrogels based on glycyrrhizin, alginate, and calcium for three‐dimensional cell culture in liver tissue engineering

Xiao-fang

Zhao

Ren

et al. 2018

J Biomedical Materials Res

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Injectable hydrogels have been paid more attentions on cell therapy and tissue regeneration resulting from the applications in minimally invasive surgical procedures with ease of handling and complete filling of defect area. Here, a biodegradable and injectable in situ hydrogel formed by glycyrrhizin (GL), alginate (Alg), and calcium (Ca) was developed for three‐dimensional (3D) cell culture. Differential scanning calorimetry (DSC), X‐ray diffraction (XRD), scanning electron microscope (SEM) and rheology analysis were performed to characterize GL‐Alg‐Ca hydrogel and evaluate its formation mechanism, properties, and morphology. The biocompatibility of hydrogel was investigated by cell viability, morphology, and liver specific functions. The results of DSC, XRD, and rheology suggested that hydrogel was homogenous complex with stable structure and well viscoelasticity. Human hepatoma HepG2 cells cultured in hydrogels showed well morphology. Compared with the control group, cells in hydrogels showed good biocompatibility, and could maintain the viability, proliferation and liver function for longer periods of time. Furthermore, the hydrogel improved the mRNA expression of cytochrome P450, which were key enzyme to the metabolization of hepatocytes. The GL‐Alg‐Ca hydrogel could be a potential 3D cells culture system for liver tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3292–3302, 2018.

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“…Cell-and material-based strategies to bone regeneration have been successfully applied in the clinic [7], yet most approaches involve pre-formed scaffolds that require invasive surgery for implantation. Minimally-invasive delivery of cells and materials is preferable to minimize surgical complications and the possibility of injection [8]. In particular, delivery of moldable materials via injection has the advantage that they can conformally fill even irregularly-shaped defects.…”

mentioning

confidence: 99%

Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects

Annamalai

Hong

Schott

et al. 2018

Preprint

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<150 Words):Repair of complex fractures with bone loss requires a potent, space-filling intervention to promote regeneration of bone. We present a minimally-invasive strategy combining mesenchymal stromal cells (MSC) with a chitosan-collagen matrix to form modular microtissues designed for delivery through a needle to conformally fill cavital defects. Implantation of microtissues into a calvarial defect in the mouse showed that osteogenically pre-differentiated MSC resulted in complete bridging of the cavity, while undifferentiated MSC produced mineralized tissue only in apposition to native bone. Decreasing the implant volume reduced bone regeneration, while increasing the MSC concentration also attenuated bone formation, suggesting that the cell-matrix ratio is important in achieving a robust response. Conformal filling of the defect with microtissues in a carrier gel resulted in complete healing. Taken together, these results show that modular microtissues can be used to augment the differentiated function of MSC and provide an extracellular environment that potentiates bone repair. Introduction:Bone has a remarkable capacity to regenerate through carefully orchestrated, cell-mediated repair processes [1]. However, healing in large and complex fractures is often impaired, leading to incomplete or functionally inferior bone regeneration. In some wounds, loss of the native vasculature and infection of the wound bed can further impair bone regeneration, resulting in a variety of pathologies, including delayed-, mal-, and non-unions. In such cases, therapeutic intervention to stimulate and accelerate the healing response is required. Bone grafting is a standard approach to this problem but needs invasive surgery to harvest and deliver the graft.Autologous grafts and flaps are limited in supply and can cause donor-site morbidity, including infection, hematoma, and pain [2]. Moreover, they are not suitable in 10-30% of cases due to difficulty in conforming the graft to the shape of the defect [3]. Allogeneic decellularized grafts and synthetic ceramic substitutes can also be used, but are biologically inferior compared to viable bone grafts due to the lack of cellular components. Although processes such as irradiation and lyophilization can reduce the risk of disease transmission from an allogeneic graft, they eliminate cellular components resulting in reduced osteoinductivity [4] and revascularization, resulting in higher bone resorption [5].The ideal bone substitute would exhibit osteoconductive, osteoinductive, and osteogenic properties and would promote concomitant neovascularization of larger defects [4]. Materialsbased approaches have been developed to promote osteoconductivity [1], and the immobilization and release of growth factors can be used as an osteoinductive cue. However, only cells can produce bone, and osteogenesis, therefore, requires either recruitment of endogenous cells or delivery of exogenous cells capable of forming bone. In large and ischemic defects, endogenous cell recruitment is impaired,...

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Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Cited by 199 publications

References 216 publications

Intelligent copolymers based on poly (N-isopropylacrylamide) PNIPAm with potential use in biomedical applications. Part i: PNIPAm functionalization with 3-butenoic acid and piperazine

Intelligent copolymers based on poly (N-isopropylacrylamide) PNIPAm with potential use in biomedical applications. Part i: PNIPAm functionalization with 3-butenoic acid and piperazine

Injectable hydrogels based on glycyrrhizin, alginate, and calcium for three‐dimensional cell culture in liver tissue engineering

Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects

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