Pulmonary immunization of chickens using non-adjuvanted spray-freeze dried whole inactivated virus vaccine completely protects against highly pathogenic H5N1 avian influenza virus

Peeters, Ben; Tonnis, Wouter F.; Murugappan, Senthil; Rottier, Peter J. M.; Koch, G.; Frijlink, Henderik W.; Huckriede, Anke; Hinrichs, Wouter L. J.

doi:10.1016/j.vaccine.2014.09.048

Cited by 12 publications

(18 citation statements)

References 22 publications

(36 reference statements)

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“…Since the 50% chicken lethal dose of the challenge virus is 2.5 log 10 EID 50 , a dose of 4.3 10logEID 50 (0.2 ml of 5.0 EID 50 /ml) is more than sufficient (> 60× CLD 50 ) to kill nonvaccinated animals. In previous studies with the same virus batch and dilution, we found that this dose killed all non-vaccinated control group animals within 2-3 days after the challenge [11,34].…”

Section: Fig 1 An Overview Of the Immunization Scheme And Immunizedmentioning

confidence: 67%

“…The virus was cultured in embryonated chicken eggs by allantoic inoculation of the seed virus. The virus was purified and inactivated as described previously to obtain whole inactivated virus vaccine (WIV) [11,28].…”

Section: Virusmentioning

confidence: 99%

“…This long term stability facilitates stockpiling and thus provides ease of administration during mass vaccination [10]. In a previous study, we have shown that pulmonary immunization by dispersion of a dry powder influenza vaccine directly at the syrinx of chickens (active administration) was able to completely protect these animals against lethal viral challenge [11]. However, in realistic situations, due to extreme time pressures, active administration of chickens is unsuitable for mass vaccination.…”

Section: Introductionmentioning

confidence: 99%

“…A more realistic approach would be to let chickens inhale aerosolized dry powder influenza vaccine formulation during breathing (passive administration). Indeed, passive inhalation of dry powder influenza vaccine formulation was feasible in chickens, but the animals were only partially protected indicated by a delay in time to death and reduced virus shedding [11]. Hence, the efficiency of influenza immunization by passive inhalation must be improved for pulmonary vaccination to become feasible.…”

Section: Introductionmentioning

confidence: 99%

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Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

Tomar

Biel

Haan

et al. 2018

European Journal of Pharmaceutics and Biopharmaceutics

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A B S T R A C TBird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the nonadjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.

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Section: Fig 1 An Overview Of the Immunization Scheme And Immunizedmentioning

confidence: 67%

Section: Virusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

Tomar

Biel

Haan

et al. 2018

European Journal of Pharmaceutics and Biopharmaceutics

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“…Influenza vaccines based on inactivated and live-attenuated virus are widely developed, and some of them are in clinical trials [37-39]. Intranasal inoculation of mice, pigs, horses and macaques with NS1-modified influenza virus strains was shown to induce robust humoral and cellular immune responses and generate protective immunity against challenge with wild-type virus [40].…”

Section: Influenza Vaccinesmentioning

confidence: 99%

Advancements in the development of subunit influenza vaccines

Zhang

Zheng

et al. 2015

Microbes and Infection

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The ongoing threat of influenza epidemics and pandemics has emphasized the importance of developing safe and effective vaccines against infections from divergent influenza viruses. In this review, we first introduce the structure and life cycle of influenza A viruses, describing major influenza A virus-caused pandemics. We then compare different types of influenza vaccines and discuss current advancements in the development of subunit influenza vaccines, particularly those based on nucleoprotein (NP), extracellular domain of matrix protein 2 (M2e) and hemagglutinin (HA) proteins. We also illustrate potential strategies for improving the efficacy of subunit influenza vaccines.

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Prime-boost vaccination strategy against avian influenza and Newcastle disease viruses reduces shedding of the challenge viruses

et al. 2018

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In the present study, we carried-out assessment of efficacy of different immunization strategies using two bivalent vaccine formulations containing antigens of inactivated Newcastle disease virus (NDV-genotype VIId) and reassortant highly pathogenic avian influenza virus (H5N1-HPAIV) mixed with Montanide ISA71 and Montanide Gel02 as adjuvants. The efficacy of the prepared vaccines was evaluated by determining the cellular and humoral immune responses. In addition, protection against H5N1-AIV and NDV-genotype VIId challenge viruses post vaccination was assessed when Montanide-Gel02 based vaccine was inoculated in 10-days-old specific pathogen free chicks intraocularly once, twice or once followed by a boost with the Montanide ISA71 based vaccine. The cytokines profile analysis demonstrated that the prime-boost strategy induced the highest up-regulation in interferon-gamma (11.39-fold change) and interleukin-6 (14.12-fold change) genes expression. Also, enhanced lymphocytes proliferation was recorded beside increased antibody titers with protection levels reaching 50 and 60% against H5N1 and NDV challenge; respectively. Immunization with Montanide ISA71 inactivated vaccine induced 80% protection; however, the prime-boost combination afforded complete protection (100%) in the challenged chickens against mortality, clinical signs and virus shedding. Finally, these results highlight the significance of considering not only different vaccine platforms but also vaccination strategies to maximize protection against AIV and NDV with regards to the longevity of the vaccine-induced immune response.

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Pulmonary immunization of chickens using non-adjuvanted spray-freeze dried whole inactivated virus vaccine completely protects against highly pathogenic H5N1 avian influenza virus

Cited by 12 publications

References 22 publications

Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

Advancements in the development of subunit influenza vaccines

Prime-boost vaccination strategy against avian influenza and Newcastle disease viruses reduces shedding of the challenge viruses

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