PST-Gold nanoparticle as an effective anticancer agent with immunomodulatory properties

Joseph, Manu M.; Aravind, S.R.; Varghese, Sheeja; Mini, S.; Sreelekha, T.T.

doi:10.1016/j.colsurfb.2012.11.046

Cited by 63 publications

(77 citation statements)

References 29 publications

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“…IC 50 value of AuNPs was determined as 5.14 μM concentration against HL-60 cells and 113.25 μM concentration against peripheral blood mononuclear cell [40]. The IC 50 concentration of polysaccharide-coated nanoparticles at IC 50 dose of 70.3 ± 1.2 μg/ml acts effectively against breast cancer cell line MCF7 and 48.9 ± 1.8 μg/ml concentration against leukemia cell line K562 [8]. The high concentration of AuNPs showed excellent cytotoxic activity against cervical cancer cell lines and also did not affect normal cells [41].…”

Section: Discussionmentioning

confidence: 99%

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Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

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The present study reports the chemical synthesis of gold nanoparticles (AuNPs) using sodium borohydride as a reducing and capping agent. The various temperatures and time intervals were investigated for controlled formation of AuNPs. The characterization of AuNPs was studied using UV-vis spectrophotometer, transmission electron microscope (TEM), selected area diffraction (SAED), energy-dispersive X-ray spectrophotometer (Edx), dynamic light scattering (DLS), zeta potential, and X-ray powder diffraction (XRD), and results indicated that AuNPs were spheroid in shape and cubic lattice and face centered in nature with 29 nm in size. AuNPs have potential antimicrobial activity against selected human clinical pathogens. IC 50 concentration of AuNPs was determined as 20 μg/ml against non-small cell lung cancer (NSCLC) cell lines using MTT assay. The morphological and apoptosis effects of AuNPs on NSCLC were investigated using acridine orange/ethidium bromide (AO/EB), 2′,7′-dichlorofluorescein-diacetate (DCFH-DA), rhodamine 123, and 4′,6-diamidino-2-phenylindole (DAPI) staining method, and results indicated that AuNPs have potential anticancer activity against NSCLC cell lines. The anticancer activity of AuNPs is that it has less toxicity against human HBL100 normal cells. These anticancer potentials of AuNPs were compared with the standard drug cisplatin. Moreover, after 24 h exposure, AuNPs arrest the cell cycle at G2/M phase in A549 cells. These findings suggested that AuNPs could act as a potential anticancer agent against lung cancer.

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Section: Discussionmentioning

confidence: 99%

“…In recent times, AuNPs have been materialized as a striking drug for targeted delivery of numerous therapeutic agents owing to its static nature, constancy, less toxicity, and biocompatibility [3,8,9]. These excellent properties of AuNPs depend upon size and shape that are controlled by various reducing and stabilizing agents [10].…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

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“…However, the lower concentrations (10, 100, and 500 g/mL) did not induce mitotic inhibition. It could be attributed to the fact that the two cell lines are more sensitive towards nanoparticles as compared to peripheral lymphocytes, thus even lower concentrations of 250-500 g/mL of nanoparticles exhibit anti-proliferative effect [38]. This might further substantiate the results of WST-1 assay where only 1000 g/mL concentration was found to exert cytotoxicity.…”

Section: Resultsmentioning

confidence: 75%

In-vitro assessment of cytotoxicity of halloysite nanotubes against HepG2, HCT116 and human peripheral blood lymphocytes

Ahmed

Shoaib

Azhar

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…Joseph et al (2013) only reported an increase in the percentage of CD3+, CD4+ and CD8+ lymphocytes in mice given, intraperitoneally, AuNPs coated with PST polysaccharide. It is unknown, however, to what extent this was caused by the nanogold itself and to what extent the reason was the plant PST polysaccharide, as the latter possesses verified anticancer and immunomodulating properties.…”

Section: Discussionmentioning

confidence: 99%

“…The available literature contains few reports on this subject. Joseph et al (2013) only demonstrated increased proliferation of peripheral blood lymphocytes in mice caused by AuNPs; however, the gold nanoparticles they used were coated with immunomodulatory PST polysaccharide. In turn, Dokić et al (2012) observed a decrease in the proliferative response of immune cells in rats due to AuNPs, but their experiment was conducted in vitro on ConA stimulated splenocytes and not on peripheral blood mononuclear cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of oral administration of commercial gold nanocolloid on peripheral blood leukocytes in mice

Małaczewska¹

2015

Polish Journal of Veterinary Sciences

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During the last few decades, owing to their unique properties, gold nanoparticles (AuNPs) have found numerous biomedical applications. Studies on rodents prove that AuNPs entering an organism easily reach the bloodstream and undergo wide tissue distribution. The presence of nanoparticles inside blood and bone marrow cells of exposed animals may implicate its influence on hematopoesis and the functions of peripheral blood leukocytes.The aim of this study was to determine the effect of oral administration of commercial gold nanocolloid, recommended by the producer as a dietary supplement, on the percentage of lymphocyte populations and proliferative response, as well as the activity of phagocytes in the peripheral blood of mice. The colloid was given to the animals in three different doses (0.25, 2.5, 25 ppm), for three different time periods (7, 14, 28 days). Mice given nanoparticles showed increased activity of phagocytes and some changes in the lymphocyte phenotypes. The elevated activity of granulocytes and monocytes, in terms of both phagocytic and respiratory burst activity, was transient and noticed only after a short time of administration, which may indicate some adaptability of blood phagocytes to prolonged presence of gold nanoparticles in the body. However, phenotypic modifications among lymphocytes in the group of animals given the middle dose of colloid (i.e. increased percentage of B and CD4+CD8+ DP T cells) did not occur until after the 28-day administration, which in turn seems indicative of some immune dysregulation due to the prolonged contact with nanogold.

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PST-Gold nanoparticle as an effective anticancer agent with immunomodulatory properties

Cited by 63 publications

References 29 publications

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

In-vitro assessment of cytotoxicity of halloysite nanotubes against HepG2, HCT116 and human peripheral blood lymphocytes

Effect of oral administration of commercial gold nanocolloid on peripheral blood leukocytes in mice

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