In-vitro assessment of cytotoxicity of halloysite nanotubes against HepG2, HCT116 and human peripheral blood lymphocytes

Ahmed, Farrukh Rafiq; Shoaib, Mohammad; Azhar, Mudassar; Um, Soong Ho; Yousuf, Rabia Ismail; Hashmi, Shahkamal; Dar, Ahsana

doi:10.1016/j.colsurfb.2015.07.021

Cited by 67 publications

(37 citation statements)

References 33 publications

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“…Kaolin, a platy sheet silicate with similar chemical composition as HNTs, has been shown to induce cytotoxicity and genotoxicity in isolated rat alveolar macrophages (Gao et al, 2000). Unmodified HNTs have shown to induce very low cytotoxicity in the following human cell types: carcinoma cells, peripheral blood lymphocytes, primary umbilical vein endothelial cells, intestinal cells, and epithelial cells (Ahmed et al, 2015;Vergaro et al, 2010;Nan et al, 2008;Lai et al, 2013). However, as HNTs have similar dimensions as short carbon nanotubes (CNTs), they may potentially cause pulmonary inflammation and acute phase response following pulmonary exposure as previously reported for CNTs (Saber et al, 2013(Saber et al, , 2014Poulsen et al, 2015Poulsen et al, , 2017Jaurand, 2017).…”

Section: Introductionmentioning

confidence: 79%

Occupational exposure during handling and loading of halloysite nanotubes – A case study of counting nanofibers

et al. 2018

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A B S T R A C THalloysite nanotubes (HNTs) are abundant naturally-occurring hollow aluminosilicate clay mineral fibers with a typical diameter < 100 nm and an aspect ratio of up to 200. Here we assessed the potential inhalation exposure to HNTs in an industrial research laboratory. Inside a fume hood, ten times 100 g of HNTs were poured at rate of 0.5 kg min . Inside the fume hood, the respirable mass concentration was 143 μg m −3 including background particles.Outside the fume hood we did not measure elevated concentrations. We classified 1895 particles according to their length and aspect ratio. Five particles were in aspect ratio > 3 and in length > 2 μm. These particles were agglomerated and/or aggregated particles where the longest individual fiber was 2 μm in length. The occupational exposure limits for refractory mineral fibers vary from 0.1 to 2 fibers cm −3. Following standard protocols for fiber analysis, detection of 0.1 fibers cm −3 would require analysis on 4 × 10 4 images when the filter loading is good. Thus, the fiber sampling and quantification procedures needs to be improved significantly if nanofibers < 100 nm in diameter are included in regulatory exposure assessment. Due to very limited toxicological information of HNTs we recommend avoiding inhalation exposure.

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Section: Introductionmentioning

confidence: 79%

Occupational exposure during handling and loading of halloysite nanotubes – A case study of counting nanofibers

et al. 2018

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“…Several studies were performed in order to assess the cytotoxicity of HNT for the potential of oral dosage. In particular Shaib et al 34 studied, by means of WST-1 assay, the effect of the nanomaterial on two cell lines, namely the colorectal carcinoma cells HCT116 and the hepatocarcinoma cells HepG2. In addition, the authors studied the cytogenetic toxicity of HNT by determining its activity against human peripheral lymphocytes by means of the mitotic index assay.…”

Section: Halloysite Biocompatibilitymentioning

confidence: 99%

Covalently modified halloysite clay nanotubes: synthesis, properties, biological and medical applications

et al. 2017

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a Halloysite (HNT) is a promising natural nanosized tubular clay mineral that has many important uses in different industrial fields. It is naturally occurring, biocompatible, and available in thousands of tons at low cost. As a consequence of a hollow cavity, HNT is mainly used as nanocontainer for the controlled release of several chemicals. Chemical modification of both surfaces (inner lumen and outer surface) is a strategy to tune the nanotube's properties. Specifically, chemical modification of HNT surfaces generates a nanoarchitecture with targeted affinity through outer surface functionalization and drug transport ability from functionalization of the nanotube lumen. The primary focus of this review is the research of modified halloysite nanotubes and their applications in biological and medical fields.

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“…Human beings have a high chance of exposure to HNTs in the contaminated environment and some products. The toxicology of HNTs has been investigated extensively in vitro, and their results indicate that HNTs are a good candidate for household materials and medicine . However, the preliminary data about the in vivo toxicity of HNTs are very limited.…”

Section: Introductionmentioning

confidence: 99%

Halloysite nanotubes‐induced Al accumulation and oxidative damage in liver of mice after 30‐day repeated oral administration

Wang

Gong

Gui

et al. 2018

Environmental Toxicology

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Halloysite (Al Si O (OH) ·nH O) nanotubes (HNTs) are natural clay materials and widely applied in many fields due to their natural hollow tubular structures. Many in vitro studies indicate that HNTs exhibit a high level of biocompatibility, however the in vivo toxicity of HNTs remains unclear. The objective of this study was to assess the hepatic toxicity of the purified HNTs in mice via oral route. The purified HNTs were orally administered to mice at 5, 50, and 300 mg/kg body weight (BW) every day for 30 days. Oral administration of HNTs stimulated the growth of the mice at the low dose (5 mg/kg BW) with no liver toxicity, but inhibited the growth of the mice at the middle (50 mg/kg BW) and high (300 mg/kg BW) doses. In addition, oral administration of HNTs at the high dose caused Al accumulation in the liver but had no marked effect on the Si content in the organ. The Al accumulation caused significant oxidative stress in the liver, which induced hepatic dysfunction and histopathologic changes. These findings demonstrated that Al accumulation-induced oxidative stress played an important role in the oral HNTs-caused liver injury.

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In-vitro assessment of cytotoxicity of halloysite nanotubes against HepG2, HCT116 and human peripheral blood lymphocytes

Cited by 67 publications

References 33 publications

Occupational exposure during handling and loading of halloysite nanotubes – A case study of counting nanofibers

Occupational exposure during handling and loading of halloysite nanotubes – A case study of counting nanofibers

Covalently modified halloysite clay nanotubes: synthesis, properties, biological and medical applications

Halloysite nanotubes‐induced Al accumulation and oxidative damage in liver of mice after 30‐day repeated oral administration

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