Pseudomonas Aeruginosa Biofilm Formation and Quorum Sensing lasR Gene in Patients with Wound Infection

El-Khashaab, Taghreed H; Erfan, Dina M.; Kamal, Ahmed A. M.; El-Moussely, Lamiaa M.; Ismail, Dalia Kadry

doi:10.12816/0037098

Cited by 9 publications

(10 citation statements)

References 30 publications

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“…Moreover, another study showed that the lowest antibiotic resistance was to imipenem with 7%, while the highest resistance was to amikacin-48% [37]. In another study in Egypt, it was found that most of the P. aeruginosa isolates were resistant to ceftazidime followed by levofloxacin and imipenem but with a lower resistance to amikacin [38]. The variation in the resistance rates among previous studies can be due to the differences in geographical distribution, time of sample collection, number of isolates gathered in each study, and the difference in antibiotic strategies adopted in every region.…”

Section: Discussionmentioning

confidence: 97%

“…For example, Abdelraheem et al, found that biofilm formation was observed in 27% Pseudomonas clinical isolates; 14% developed strong biofilm, 7% developed moderate biofilm, and 6% developed weak biofilm [37]. A different study in Egypt demonstrated that biofilm formation was observed in 91.4% P. aeruginosa isolates; 25.7%, 40%, 25.7%, and 8.6% of isolates were strong, moderate, weak, and nonbiofilm producers, respectively [38]. The variation in findings between different studies can be related to several variables, including the differences in type and number of strains gathered in each research study, that eventually lead to differences in the ability of isolates to produce biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa

et al. 2020

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Biofilm acts as a complex barrier against antibiotics. In this study, we investigated the inhibitory activities of Olea europaea (olive) leaves Camellia sinensis (green tea), Styrax benzoin, Ocimum basilicum, Humulus lupulus, Ruta graveolens, and Propolis extracts on the biofilm formation, pyocyanin production, and twitching motility of Pseudomonas aeruginosa isolates. Moreover, we investigated the effect of olive leaf extract on the transcription of some biofilm related genes. A total of 204 isolates of Pseudomonas were collected from different Egyptian hospitals. A susceptibility test, carried out using the disc diffusion method, revealed that 49% of the isolates were multidrug-resistant. More than 90% of the isolates were biofilm-forming, of which 26% were strong biofilm producers. At subinhibitory concentrations, green tea and olive leaf extracts had the highest biofilm inhibitory effects with 84.8% and 82.2%, respectively. The expression levels of lasI, lasR, rhlI, and rhlR treated with these extracts were significantly reduced (p < 0.05) by around 97–99% compared to untreated isolates. This study suggests the ability of olive leaf extract to reduce the biofilm formation and virulence factor production of P. aeruginosa through the down regulation of quorum sensing (QS) genes. This may help in reducing our dependence on antibiotics and to handle biofilm-related infections of opportunistic pathogens more efficiently.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa

et al. 2020

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“…The most commonly studied bacteria capable of forming biofilms are A. baumannii, P. aeruginosa and S. maltophilia. Biofilm formation is important in establishing infections on different host tissues as well as different medical devices in hospital acquired infections in Egyptian hospitals ICUs [7]. In these settings, the antibiotic resistance engendered by biofilms presents a serious challenge to the treatment of nosocomial infections caused by MDR bacteria [7].…”

Section: Tissue Culture Plate Methods (Tcp)mentioning

confidence: 99%

“…One of the most common biofilm forming bacteria is P. aeruginosa [7]. P. aeruginosa has been extensively studied for genetic determinants that contribute to form biofilm.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Production by Multi Drug Resistant Bacterial Pathogens Isolated From Patients in Intensive Care Units in Egyptian Hospitals

Elhabibi¹,

Ramzy²

2017

J Microb Biochem Technol

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“…The fold change in gene expression was normalized to the reference gene (pro C) and relative to the control sample. Then the relative expression was con rmed by using free data analysis tools [11]. PCR products were analyzed by gel electrophoresis, to exclude any unspeci c products are present.…”

Section: Rt-pcrmentioning

confidence: 99%

The effect of Zinc Oxide Nanoparticles on Pseudomonas Aeruginosa Biofilm Formation and Virulence Genes Expression

Abdelraheem

Mohamed

2020

Preprint

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Abstract Background: Due to increased resistance to antimicrobial agents, infectious disease remains a public health problem worldwide. Aim: The current study was designed to examine the effect of ZnO nanoparticles (ZnO –np) against the biofilm formation ability of P.aeruginosa clinical isolates and to study its effect on the expression level of the genes involved in biofilm formation and virulence factors production. Methodology: The MIC of ZnO –np against P. aeruginosa was determined by the broth microdilution method. The Effect of ZnO –np on the biofilm-forming isolates of P. aeruginosa was monitored by the microtiter plate method. P.aeruginosa isolates were tested for the expression of different biofilm and virulence genes using real-time rt-PCR. Results: ZnO –np significantly downregulated the expression level of all biofilm and virulence genes of P.aeruginosa clinical isolates except the toxA gene. Conclusions: This study demonstrates the promising use of ZnO –np as an anti-biofilm and anti-virulence compound.

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Pseudomonas Aeruginosa Biofilm Formation and Quorum Sensing lasR Gene in Patients with Wound Infection

Cited by 9 publications

References 30 publications

Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa

Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa

Biofilm Production by Multi Drug Resistant Bacterial Pathogens Isolated From Patients in Intensive Care Units in Egyptian Hospitals

The effect of Zinc Oxide Nanoparticles on Pseudomonas Aeruginosa Biofilm Formation and Virulence Genes Expression

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