Biofilm Production by Multi Drug Resistant Bacterial Pathogens Isolated From Patients in Intensive Care Units in Egyptian Hospitals

Elhabibi, T; Ramzy, S

doi:10.4172/1948-5948.1000359

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…In our study, among strong biofilm producers, P. aeruginosa was 85.7% followed by A. baumannii 14.29% [Table 2 &11] which is correlating with the above study. Several studies showed that there is100% association of bap gene was seen among the clinical isolates of A. baumannii and also 100% association of Rhys gene with clinical isolates of P.aeruginosa 12 , in our study, bap gene association with A. baumannii was found to be 100% which is correlating with the above study, whereas the RhLI gene was detected only in 50% P.…”

Section: Discussionsupporting

confidence: 89%

Molecular Methods for Detecting Biofilm Producing Multidrug Resistant Clinical Isolates

Bankole¹,

Vasanthi²,

Murugesan³

et al. 2020

ATMPH

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Background: Biofilm formation is known to be a major virulence factor in chronic infections caused by both gram-positive and gram-negative bacteria. It has also been discovered that biofilm-forming organisms are usually associated with multidrug resistance. Aims and objectives: Detection of biofilm formation among multidrug-resistant organisms by phenotypic and genotypic methods. Materials and methods: A total of forty isolates obtained from various clinical samples were subjected for antimicrobial susceptibility testing by Kirby-Bauer disc diffusion test and E strip method, these isolates were screened for biofilm formation by phenotypic tissue culture plate method. The isolates forming strong biofilm were evaluated by conventional PCR for multidrug-resistant genes and biofilmproducing genes. Results: Among the 40 isolates obtained, 16 showed the formation of biofilm by phenotypic method, out of these 7 which are strong biofilm producers and 4 showed the presence of biofilm-producing genes and 6 multidrug-resistant genes. Conclusion: A significant association is seen among the clinical isolates with multidrug resistance and biofilm formation which makes it necessary to screen all the clinical isolates for the same for better treatment options.

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Section: Discussionsupporting

confidence: 89%

Molecular Methods for Detecting Biofilm Producing Multidrug Resistant Clinical Isolates

Bankole¹,

Vasanthi²,

Murugesan³

et al. 2020

ATMPH

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Characterization of Uropathogenic Pseudomonas aeruginosa: Serotypes, Resistance Phenotypes, and Virulence Genotypes

Elbargisy¹

2022

J. Pure Appl. Microbiol.

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Pseudomonas aeruginosa is a major cause of urinary tract infections. This organism has extended resistance to antimicrobials along with multiple virulence factors, making it difficult to treat. In this study, 49 isolates from urine samples were identified as P. aeruginosa and serotyped by the slide agglutination method. The sensitivity of isolates against 10 antipseudomonal drugs was determined. Phenotypically, lipase, protease, hemolysin, and biofilm production were detected. Genes for the type III secretion system, elastase B, and exotoxin A were detected by PCR. Serotype O11 was the most predominant serotype among test isolates. High levels of resistance were observed against ceftazidime, cefepime, piperacillin, and piperacillin/tazobactam while 10.2% of isolates were resistant to amikacin. MDR was detected in 20.4% of the isolates and was significantly associated with strong biofilm producers. About 95.9% and 63.3% of P. aeruginosa isolates had proteolytic and lipolytic activity, respectively. Among the genes detected, the exoY gene was the most prevalent gene (79.6%), while the exoU gene was the least frequent one (10.2%). toxA and lasB genes were amplified in 63.27% and 75.5% of the isolates, respectively. In addition, the exoU gene was significantly associated with MDR isolates. The high incidence of exoS, exoT, exoY, lasB, and toxA genes in uropathogenic P. aeruginosa implies that these genes can be considered markers for virulent isolates. Furthermore, the coexistence of exoU and exoS genes, even in 6% of isolates, poses a significant treatment challenge because those isolates possess both the invasive and cytotoxic properties of both effector proteins.

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Biofilm Production by Multi Drug Resistant Bacterial Pathogens Isolated From Patients in Intensive Care Units in Egyptian Hospitals

Cited by 2 publications

References 25 publications

Molecular Methods for Detecting Biofilm Producing Multidrug Resistant Clinical Isolates

Molecular Methods for Detecting Biofilm Producing Multidrug Resistant Clinical Isolates

Characterization of Uropathogenic Pseudomonas aeruginosa: Serotypes, Resistance Phenotypes, and Virulence Genotypes

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