Pseudoalteromonas marina sp. nov., a marine bacterium isolated from tidal flats of the Yellow Sea, and reclassification of Pseudoalteromonas sagamiensis as Algicola sagamiensis comb. nov.

Nam, Young-Do; Chang, Ho-Won; Park, Ja Ryeong; Kwon, Hee Jin; Quan, Zhe‐Xue; Park, Yong-Ha; Lee, Jung-Sook; Yoon, Jung‐Hoon; Bae, Jin‐Woo

doi:10.1099/ijs.0.64523-0

Cited by 46 publications

(21 citation statements)

References 23 publications

(29 reference statements)

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“…There were Cobetia marina (Arahal et al 2002), Colwellia aestuarii (Jung et al 2006), Endozoicimonas elysicola (Kurahashi and Yokoto 2006), Pseudoalteromonas aliena (Ivanova et al 2004), Pseudoalteromonas marina (Nam et al 2007), P. tetraodonis (Ivanova et al 2001), Psychrobacter nivimaris (Heuchert et al 2004), Shewanella livingstonensis (Bozal et al 2002), S. sairae (Satomi et al 2003), S. surugaensis (Miyazaki et al 2006), S. violacea (Nogi et al 1998). Their physiological traits are only partially known, yet.…”

Section: Phylogenetic Analysis Of Mixed Culturesmentioning

confidence: 99%

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Schuett

Doepke

2009

Helgol Mar Res

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Endobiotic bacteria colonize the tentacles of cnidaria. This paper provides first insight into the bacterial spectrum and its potential of pathogenic activities inside four cnidarian species. Sample material originating from Scottish waters comprises the jellyfish species Cyanea capillata and C. lamarckii, hydrozoa Tubularia indivisa and sea anemone Sagartia elegans. Mixed cultures of endobiotic bacteria, pure cultures selected on basis of haemolysis, but also lyophilized samples were prepared from tentacles and used for DGGE-profiling with subsequent phylogenetic analysis of 16S rDNA fragments. Bacteria were detected in each of the cnidarian species tested. Twenty-one bacterial species including four groups of closely related organisms were found in culture material. The species within these groups could not be differentiated from each other (one group of Pseudoalteromonas spp., two groups of Shewanella spp., one group of Vibrio spp.). Each of the hosts exhibits a specific endobacterial spectrum. Solely Cyanea lamarckii harboured Moritella viscosa. Only in Cyanea capillata, members of the Shewanella group #2 and the species Pseudoalteromonas arctica, Shewanella violacea, Sulfitobacter pontiacus and Arcobacter butzleri were detected. Hydrozoa Tubularia indivisa provided an amazingly wide spectrum of nine bacterial species. Exclusively, in the sea anemone Sagartia elegans, the bacterial species P. aliena was found. Overall eleven bacterial species detected were described recently as novel species. Four 16S rDNA fragments generated from lyophilized material displayed extremely low relationship to their next neighbours. These organisms are regarded as members of the endobiotic ''terra incognita''. Since the origin of cnidarian toxins is unclear, the possible pathogenic activity of endobiotic bacteria has to be taken into account. Literature data show that their next neighbours display an interesting diversity of haemolytic, septicaemic and necrotic actions including the production of cytotoxins, tetrodotoxin and R-toxin. Findings of haemolysis tests support the literature data. The potential producers are Endozoicimonas elysicola, Moritella viscosa, Photobacterium profundum, P. aliena, P. tetraodonis, Shewanella waksmanii, Vibrio splendidus, V. aestuarius, Arcobacter butzleri.

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Section: Phylogenetic Analysis Of Mixed Culturesmentioning

confidence: 99%

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Schuett

Doepke

2009

Helgol Mar Res

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“…Physiological and biochemical characteristics were determined at 30 u C using the API 20 NE kit (bioMérieux) according to the manufacturer's instructions with the exception that suspensions were prepared in a 50 : 50 (v/v) mixture of artificial seawater and AUX medium (bioMérieux) (Nam et al, 2007 MIDI System (Sherlock Microbial Identification System, version 6.1). The cellular fatty acid profiles of strains GH2-4 T and GH2-5 contained iso-C 15 : 0 (57-58 %), iso-C 16 : 0 (8-10 %), anteiso-C 15 : 0 (7 %) and iso-C 14 : 0 (6 %) as major fatty acids.…”

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confidence: 99%

“…Physiological and biochemical characteristics were determined at 30 u C using the API 20 NE kit (bioMérieux) according to the manufacturer's instructions with the exception that suspensions were prepared in a 50 : 50 (v/v) mixture of artificial seawater and AUX medium (bioMérieux) (Nam et al, 2007). The artificial seawater contained (per litre distilled water) 23.6 g NaCl, 0.64 g KCl, 4.53 g MgCl 2 .…”

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confidence: 99%

Bacillus solimangrovi sp. nov., isolated from mangrove soil

Lee

Rhee

Chang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Two novel bacterial strains, GH2-4 T and GH2-5, were isolated from mangrove soil near the seashore of Weno island in Chuuk state, Micronesia, and were characterized by a polyphasic approach. The two strains were strictly aerobic, Gram-staining-positive, motile, endospore-forming rods that were catalase-and oxidase-positive. Colonies were circular, convex, stringy and transparent yellowish (GH2-4 T ) or opaque whitish (GH2-5). The 16S rRNA gene sequences of the two isolates were identical. The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus kochii WCC 4582 T , B. horneckiae DSM 23495 T , B. azotoformans LMG 9581 T , B. cohnii DSM 6307 T and B. halmapalus DSM 8723 T (95.6, 95.4, 95.4, 95.2 and 95.2 % similarity, respectively). The partial groEL sequence of strain GH2-4 T was identical to that of strain GH2-5 and showed ,85 % similarity to those of the most closely related strains. The isolates grew at pH 5-12 (optimal growth at pH 9), at 10-40 6C (optimum 30-35 6C) and at 0-9 % (w/v) NaCl (optimum 1-3 % NaCl). The cell-wall peptidoglycan of strains GH2-4 T and GH2-5 contained meso-diaminopimelic acid and cell-wall hydrolysates contained ribose as a major sugar. The DNA G+C content was 36 mol%, and DNA-DNA relatedness between the isolates and five related reference strains was 20-24 %. Strain GH2-4 T exhibited 81 % DNA-DNA relatedness with strain GH2-5. The major cellular fatty acids of both strains were iso-C 15 : 0 , iso-C 16 : 0 , iso-C 14 : 0 and anteiso-C 15 : 0 and the predominant menaquinone was MK-7. On the basis of the evidence from this polyphasic study, strains GH2-4 T and GH2-5 (5KCTC 331435JCM 189955DSM 27084) represent a novel species of the genus Bacillus, for which the name Bacillus solimangrovi sp. nov. is proposed; the type strain is GH2-4 T (5KCTC 33142 T 5JCM 18994 T 5DSM 27083 T ).Mangroves are woody plants located at the interface between land and sea. They represent a typical ecosystem in tropical and subtropical coastal biomes, and they play significant roles as feeding, breeding and refuge areas for diverse organisms and maintain a considerable food web based on their detritus (Holguin et al., 2001;Kathiresan & Bingham, 2001). The mangrove ecosystem is distinguished from others because of periodic tidal flooding and changeable environmental factors, such as salinity and nutrient availability (Alongi, 1988;Holguin et al., 2006). There have been many reports regarding the isolation of novel micro-organisms from mangrove-related environments. At the time of writing, 63 genera and 113 species had been newly described as novel micro-organisms isolated from mangrove environments, based on searches of PubMed (http://www.ncbi.nlm.nih. gov/). They include species of the yeast genus Candida The genus Bacillus, in the phylum Firmicutes, is a large group of rod-shaped, endospore-forming bacteria that includes more than 200 species (Euzéby, 2010). Since the genus Bacillus was described by Cohn in 1872, the number of species in the genus has increased considerabl...

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“…They are motile by a single polar flagellum and have a chemoheterotrophic metabolism (Bowman & McMeekin, 2005). At the time of writing, the genus Pseudoalteromonas comprises 34 species with validly published names (Gauthier et al, 1995;Ivanova et al, 1996Ivanova et al, , 1998Ivanova et al, , 2000Ivanova et al, , 2001 Ivanova et al, , 2002a Ivanova et al, , b, c, d, 2004Bozal et al, 1997; Euzéby, 1997;Bowman, 1998; Holmström et al, 1998; Sawabe et al, 2000;Venkateswaran & Dohmoto, 2000;Egan et al, 2001;Isnansetyo & Kamei, 2003; Romanenko et al, 2003a, b;Lau et al, 2005;Park et al, 2005;Nam et al, 2007;Al Khudary et al, 2008). Some strains produce yellow, red, violet or melanin-like pigments and have the ability to form antibiotic substances (Bowman, 2007).…”

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confidence: 99%

Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

Wang

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39 T , was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C 16 : 1 v7c/iso-C 15 : 0 2-OH, C 16 : 0 , C 18 : 1 v7c, C 12 : 0 3-OH, C 17 : 1 v8c and C 17 : 0 . The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Pseudoalteromonas. Strain LMEB 39 T exhibited the closest phylogenetic affinity to Pseudoalteromonas byunsanensis JCM 12483 T (97.4 % sequence similarity). The DNA-DNA reassociation values between strain LMEB 39 T and P. byunsanensis JCM 12483 T and Pseudoalteromonas undina DSM 6065 T (97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39 T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas lipolytica sp. nov. is proposed; the type strain is LMEB 39 T (5CGMCC 1.8499 T 5JCM 15903 T ).The genus Pseudoalteromonas was proposed by Gauthier et al. (1995) and represents a clade of marine bacteria grouped by their phylogenetic relationships based on 16S rRNA gene sequences. Members of the genus Pseudoalteromonas are Gram-negative, strictly aerobic, rod-shaped cells. They are motile by a single polar flagellum and have a chemoheterotrophic metabolism (Bowman & McMeekin, 2005). At the time of writing, the genus Pseudoalteromonas comprises 34 species with validly published names (Gauthier et al., 1995;Ivanova et al., 1996Ivanova et al., , 1998Ivanova et al., , 2000Ivanova et al., , 2001 Ivanova et al., , 2002a Ivanova et al., , b, c, d, 2004Bozal et al., 1997; Euzéby, 1997;Bowman, 1998; Holmström et al., 1998; Sawabe et al., 2000;Venkateswaran & Dohmoto, 2000;Egan et al., 2001;Isnansetyo & Kamei, 2003; Romanenko et al., 2003a, b;Lau et al., 2005;Park et al., 2005;Nam et al., 2007;Al Khudary et al., 2008). Some strains produce yellow, red, violet or melanin-like pigments and have the ability to form antibiotic substances (Bowman, 2007). Here, a novel non-pigmented Pseudoalteromonas strain isolated from the Yangtze River estuary was studied using a polyphasic approach.A seawater sample was collected in October 2007 from the Yangtze River estuary (31u 009 040 N 122u 379 260 E) near the East China Sea at a depth of 21 m (temperature 21 u C, salinity 30.5 %). The sample was stored in darkness until micro-organisms were isolated in July 2008. Approximately 80 ml seawater was plated on halophilic medium (HM) containing 10 % (w/v) NaCl by using a te...

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Pseudoalteromonas marina sp. nov., a marine bacterium isolated from tidal flats of the Yellow Sea, and reclassification of Pseudoalteromonas sagamiensis as Algicola sagamiensis comb. nov.

Cited by 46 publications

References 23 publications

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Bacillus solimangrovi sp. nov., isolated from mangrove soil

Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

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