A novel exopolysaccharide-producing bacterium, designated strain k53 T , was isolated from sediment from the Arabia Sea, Indian Ocean. The strain was Gram-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na + for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C 16 : 1 v7c, C 16 : 0 and C 18 : 1 v7c. The DNA G+C content was 43 mol%. 16S rRNA gene sequence analysis suggested that strain k53 T is a member of the genus Pseudoalteromonas. Strain k53 T exhibited close phylogenetic affinity to Pseudoalteromonas lipolytica LMEB 39 T (98.0% 16S rRNA gene sequence similarity) and Pseudoalteromonas donghaensis HJ51 T (97.3 %).The DNA-DNA reassociation values between strain k53 T and P. lipolytica JCM 15903 T and P. donghaensis LMG 24469 T were 17 % and 12 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analysis based on the 16S rRNA gene sequence and DNA-DNA relatedness data, the isolate merits classification as a representative of a novel species, for which the name Pseudoalteromonas arabiensis is proposed. The type strain of this species is k53 T (5JCM 17292 T 5NCIMB 14688 T ).There have been many reports of marine micro-organisms that produce exopolysaccharides (EPSs). It remains possible that new polysaccharide-producing bacteria could be found in various habitats, and we have been trying to find them in sediment from the ocean bed (Matsuyama et al., 2006). Strain k53 T was isolated as a polysaccharide producer and was considered to be a 'Pseudoalteromonas-like' strain. The genus Alteromonas originally described by Baumann et al. (1972) for marine aerobic, Gram-negative, non-fermentative, polarly flagellated bacteria, was later divided into two genera, Alteromonas and Pseudoalteromonas, on the basis of phylogenetic analysis (Gauthier et al., 1995). At the time of writing, the genus Pseudoalteromonas comprised 38 species and two subspecies (http://www.bacterio.cict.fr/p/pseudoalteromonas. html). Members of the genus Pseudoalteromonas have been isolated from marine environments and the genus is one of the largest within the class Gammaproteobacteria. In this study, we isolated a polysaccharide-producing bacterium 'belonging to genus Pseudoalteromonas' and the phenotypic and chemotaxonomic characteristics and results of phylogenetic analysis based on the 16S rRNA gene sequence of the isolate showed that it merited classification as a novel Pseudoalteromonas species. Sediment samples were collected from the Arabia Sea at a depth of 3615 m (16 u 459 0199 N, 69 u 009 0299 E) using a multiple corer during the GEOTRACES cruise in 2009. Strain k53 T was isolated by selective enrichment. The sediment sample was inoculated in 10 ml mineral salts medium (KJ medium) containing (l 21 ) 23.4 g NaCl, 5 g MgCl 2 , 1.1 g CaCl 2 . 2H 2 O, 0.7 g KCl, 0.2 g NaHCO 3 , 6.05 g Tris, 5 g peptone, 10 g glucose, 0.01 g K 2 HPO 4 , 0.008 g Na 2 HPO 4 , 1.0 g (NH 4 ) ...