Abstract-The total lipid contents of local sea cucumbers; Holothuria scabra Jaeger, H. leucospilota Brandt, H. atra Jaeger and Stichopus horrens Selenka were determined with the respective results on dry matter basis 0.72%, 1.42%, 0.99% and 1.55%. Meanwhile, the free fatty acids composition for each lipid extract showed all species contained arachidonic acid (C20:4n6) of polyunsaturated fatty acids (PUFA) of which S. horrens being the highest 42.41% followed by H. atra, 24.76%, H. leucospilota, 23.23% and H. scabra, 19.63%. H. scabra, H. leucospilota and H. atra were rich in palmitic acid (C16:0) of saturated fatty acids (SFA); 52.66%, 35.63% and 34.21%, respectively. Myristic acid (C14:0) of SFA was higher in S. horrens (24.36%). However, the fatty acids eicosapentanoic acid (EPA) [C20:5] were very low for all species; H. scabra (1.12%), H. leucospilota (1.34%), H. atra (0.17%) and S. horrens (0.79%). Docosahexanoic acid (DHA) was only detected in S. horrens, i.e. 0.32%. Thus, the results of this study indicated that different species of sea cucumber varies in its lipid content and free fatty acids composition.Index Terms-Lipid, free fatty acids, sea cucumbers.