Protozoological Approaches to the Cellular Basis of Mammalian Stress Repair

Hutner, S. H.; Marcus, Stuart L.

doi:10.1016/s0074-7696(08)61704-0

Cited by 4 publications

(3 citation statements)

References 228 publications

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“…It occurs in cytochromes [3]. Besides, Hutner et al emphasized that some strains excrete protoporphyrin to the medium [7]. We are aware that our Tetrahyn~ena species can indeed synthesize all of the required porphyrin rings, since the cells can be grown for any length of time without addition of these compounds.…”

Section: Discussionmentioning

confidence: 96%

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Porphyrin Rings and Phospholipids: Stimulators of Cloning Efficiency in Certain Species of Tetrahymena

Schousboe

Christensen

Ghiladi

et al. 1992

The Journal of Protozoology

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Species of Tetrahymena, including T. vorax, T. thermophila, T. pyriformis, and T. pigmentosa, were tested for cloning efficiency in proteose peptone and in synthetic nutrient media to which were added hemin, protoporphyrin IX, chlorophyllin, or asolectin, an impure mixture of phospholipids. All species could be cloned with high efficiency in the crude media. In unsupplemented synthetic medium the cloning efficiencies were 0-10%, around 50%, around 50%, and 90-100% for T. thermophila, T. vorax, T. pyriformis, and T. pigmentosa, respectively. The first three were all stimulated to 90-100% by addition of the porphyrin or phospholipid compounds mentioned above. Uroporphyrin III and coproporphyrin I and III had no effect. We suggest that cells unable to form clones suffer from a lack of cellular energy. This situation may be alleviated by our additions, certain porphyrin rings may be built into cytochromes and phospholipids may be used as fuel. Thus, the synthetic media used so far for these ciliates have not been optimal.

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Section: Discussionmentioning

confidence: 96%

“…It could also mean that it is the presence of lipids that brings about high cloning efficiency in proteose peptone solutions. Other authors have attributed stimulation of growth by phospholipids in these cells to sparing effects [7].…”

Section: Discussionmentioning

confidence: 99%

Porphyrin Rings and Phospholipids: Stimulators of Cloning Efficiency in Certain Species of Tetrahymena

Schousboe

Christensen

Ghiladi

et al. 1992

The Journal of Protozoology

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“…Here I was, helping in a small way, and getting to know the other committee members, including William Trager (President, 1960(President, -1961, Seymour Hutner (President, 1961-1962 and the Secretary-General of the Congress, John Lee (President, 1990(President, -1991. When Hutner needed some help with the editing of the proceedings of the Congress, I came to the Haskins Laboratory to help out (Hutner and Bleyman 1979.) I stayed in his laboratoiy, and while there, followed up on some ideas for easily keeping Tetrahymena in culture that had been developed independently by Norman Williams (President, 1984-1985 and Jason Wolfe (Williams, Wolfe, and Bleyman 1980).…”

Section: Leu Bleymunmentioning

confidence: 99%

The Accidental Protozoologist—My Journey Through the World of Ciliates^1,²

Bleyman

2003

J Eukaryotic Microbiology

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The Past-President's Address has been the opportunity for the speaker to reminisce about the road traveled to get to this time in life. In this paper, I continue in that tradition. During my journey to the present day, I visited different laboratories, studying the genetics of mating and mating types in Paramecium, Tetrahymena, Blepharisma and Euplotes. I have met and worked with many distinguished scientists, including other Past-Presidents of the Society. I also became an active participant in the Society of Protozoologists. I hope the recounting of my trip will be both entertaining and enlightening.

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Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis

Шемарова

Maizel

Khovanskikh

2000

J Evol Biochem Phys

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Protozoological Approaches to the Cellular Basis of Mammalian Stress Repair

Cited by 4 publications

References 228 publications

Porphyrin Rings and Phospholipids: Stimulators of Cloning Efficiency in Certain Species of Tetrahymena

Porphyrin Rings and Phospholipids: Stimulators of Cloning Efficiency in Certain Species of Tetrahymena

The Accidental Protozoologist—My Journey Through the World of Ciliates^1,²

Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis

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Protozoological Approaches to the Cellular Basis of Mammalian Stress Repair

Cited by 4 publications

References 228 publications

Porphyrin Rings and Phospholipids: Stimulators of Cloning Efficiency in Certain Species of Tetrahymena

Porphyrin Rings and Phospholipids: Stimulators of Cloning Efficiency in Certain Species of Tetrahymena

The Accidental Protozoologist—My Journey Through the World of Ciliates1,2

Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis

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The Accidental Protozoologist—My Journey Through the World of Ciliates^1,²