Prototype Single Step Lateral Flow Technology for Detection of Avian Influenza Virus and Chicken Antibody to Avian Influenza Virus

Nielsen, Kent Jacob; Wang, Yu; Lin, Ming‐Hsien; Davis, Sheree; Elmgren, C.; MacKenzie, Ross; Tanha, Jamshid; Li, S.; Dubuc, G.; Brown, Elizabeth; Keleta, Liya; Pasick, John

doi:10.1080/15321810701603443

Cited by 14 publications

(11 citation statements)

References 18 publications

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“…In this work, we characterise commercially available polystyrene nanoparticles coated with immunoglobulin G (IgG) by combining the use of particle sizing techniques such as dynamic light scattering (DLS), DCS and small‐angle X‐ray scattering (SAXS). Polystyrene nanoparticles are commonly used in the development of nanoparticle‐based strategies for medicine, thanks to the low cost of their material, the versatility of their surface functionalization, the possibility to load them with fluorescent dyes and their availability in narrow size distributions . In many of their applications, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of IgG‐protein‐coated polymeric nanoparticles using complementary particle sizing techniques

Minelli

Garcia-Diez

Sikora

et al. 2014

Surface & Interface Analysis

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When nanoparticles are introduced into biological media, they acquire a protein corona. The thickness of immunoglobulin G protein on 105 nm polystyrene particles has been determined by dynamic light scattering (DLS), differential centrifugal sedimentation (DCS) and small‐angle X‐ray scattering (SAXS). All three techniques measured an increase of the protein shell thickness with increasing concentration of the proteins during incubation with the nanoparticles. DLS measurements showed the highest increase, indicating a possible effect of particle agglomeration on the DLS results. DCS accuracy critically depends on the knowledge of the protein shell density but the data allows an estimation of the effective shell density when the thickness was independently determined. SAXS measurements revealed the complex core/shell structure of the bare polystyrene particles. © 2014 Crown Copyright. Surface and Interface Analysis © 2014 John Wiley & Sons Ltd.

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Section: Introductionmentioning

confidence: 99%

“…In many of their applications, e.g. biosensing, polystyrene nanoparticles are conjugated with biomolecules and used in biological media . The design and control of their function in these complex media requires an in depth understanding of their interface with the environment, e.g.…”

Section: Introductionmentioning

confidence: 99%

Characterization of IgG‐protein‐coated polymeric nanoparticles using complementary particle sizing techniques

Minelli

Garcia-Diez

Sikora

et al. 2014

Surface & Interface Analysis

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“…[32] Highly sensitive and specific LFAs have also been developed for detection of influenza virus in respiratory specimen from children [33 -37] and in chickens. [38] These tests provide a very rapid indication of the presence of substances of interest by a technique which requires no equipment and which can be performed under most environmental conditions. While the described LFA appears to provide accurate results, complete validation of the assay remains to be done.…”

Section: Discussionmentioning

confidence: 99%

Development of a Lateral Flow Assay for Rapid Detection of Bovine Antibody toAnaplasma marginale

Nielsen

Wang

Kelly

et al. 2007

Journal of Immunoassay and Immunochemistry

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A rapid lateral flow assay for detection of bovine antibody to Anaplasma marginale was developed. The assay used a recombinant peptide of major surface protein 5 as the antigen and a monoclonal antibody specific for bovine IgG(1) conjugated with colloidal gold beads for detection. Serum and anticoagulated blood samples were obtained from cattle in an area where anaplasmosis was endemic. The samples were selected based on positive identification of the organism in blood smears. The unclotted blood samples were used for PCR determination of the presence of A. marginale while the sera were tested by a commercial competitive enzyme immunoassay (CELISA) and by the lateral flow assay (LFA). Similar samples, collected at a Canadian sales barn, were tested by the CELISA and LFA and 10% were tested by PCR for the presence of A. marginale nucleic acid. In addition, stored serum samples from a second endemic area were tested by CELISA and LFA. Of the 114 smear positive samples, all were positive by CELISA and LFA. All samples were also positive by PCR. Samples from Canadian sources (n=524) were negative in the CELISA but 11 sera gave false positive reactions in the LFA. All samples tested were PCR negative. Of 113 samples from herds with anaplasmosis, 53 were positive in the CELISA and 50 were LFA positive.

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“…The immunoassay strip is composed of four major components: a cellulose fiber sampl e pad (Millipore, CFSP203000), a conjugate pad (Millipore, GFCP103000), a NC membrane (Millipore, HF120MC100), and an absorbent pad (Millipore, CFSP173000) (Zhang et al 2005;Nielsen et al 2007). A schematic representation of the lateral flow assay is given in Fig.…”

Section: Development Of Lateral Flow Immunoassay Strips For Detectionmentioning

confidence: 99%

Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

Zafar

Shah

Shehzad

et al. 2020

Appl Microbiol Biotechnol

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Birnaviridae is a family of viruses (birnaviruses) which consists of four genera, members of which cause diseases in fish, birds, mollusks, and insects. The genome of birnaviruses encodes the highly immunogenic VP2 capsid protein. In order to demonstrate that the VP2 protein can be exploited as a diagnostic antigen for birnaviruses, we developed a lateral flow assay based on the surface-exposed VP2 protrusion domain of a representative birnavirus, infectious bursal disease virus (IBDV) of serotype 1 which causes the highly devastating infectious bursal disease in chickens. The biophysical characterization of the purified domain reveals that the domain predominantly consists of β-sheets, exists in a trimeric form, and remains folded at high temperatures, making it suitable for diagnostic purposes. Owing to its highly immunogenic nature and excellent biophysical properties, we employed the VP2 protrusion domain in a gold nanoparticle-based lateral flow assay for rapid detection of anti-IBDV antibodies in serum samples of infected chickens. Our results indicate that the domain binds anti-IBDV antibodies with high specificity during laboratory testing and on-site testing. The lateral flow assay reported here yields comparable results in a qualitative manner as obtained through a commercial enzyme-linked immunosorbent assay (ELISA). As VP2 is a common capsid protein of birnaviruses, the lateral flow assay can be generalized for other birnaviruses, and members of Tetraviridae and Nodaviridae families which contain homologous VP2 capsid proteins. Keywords Birnavirus . Circular dichroism . Gold nanoparticle-based lateral flow assay . Infectious bursal disease virus . Infrared spectroscopy . VP2 protrusion domain Electronic supplementary material The online version of this article (https://doi.

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Prototype Single Step Lateral Flow Technology for Detection of Avian Influenza Virus and Chicken Antibody to Avian Influenza Virus

Cited by 14 publications

References 18 publications

Characterization of IgG‐protein‐coated polymeric nanoparticles using complementary particle sizing techniques

Characterization of IgG‐protein‐coated polymeric nanoparticles using complementary particle sizing techniques

Development of a Lateral Flow Assay for Rapid Detection of Bovine Antibody toAnaplasma marginale

Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

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