Prototype for Automatable, Dielectrophoretically-Accessed Intracellular Membrane–Potential Measurements by Metal Electrodes

Terpitz, Ulrich; Sukhorukov, Vladimir L.; Zimmermann, D.

doi:10.1089/adt.2012.455

Cited by 6 publications

(3 citation statements)

References 65 publications

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“…13 and 15 in Ref. 32). According to Tan et al, 37 a medaka egg experiences a critical puncture force of F p % 12.5 mN at the moment of chorion breakage, i.e., at a deformation of $400 lm.…”

Section: A Mechanical Piercing Of Medaka Eggs With a Nickel Needlementioning

confidence: 89%

“…[29][30][31] Recently, the dielectrophoretically accessed, intracellular membrane-potential measurement method has been developed for electrophysiological studies of human cells with a diameter of $15 lm. 32 DEP has also been successfully used for sorting bovine oocytes ($100 lm in diameter) according to their developmental competence. 33 In this communication, we show a proof of principle for the applicability of the DEP-based electro-microinjection technique to extra-large biological particles, such as fertilized eggs and early stage embryos of Japanese medaka fish, measuring 1.2 mm in diameter.…”

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confidence: 99%

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Electro-microinjection of fish eggs with an immobile capillary electrode

et al. 2015

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Microinjection with ultra-fine glass capillaries is widely used to introduce cryoprotective agents and other foreign molecules into animal cells, oocytes, and embryos. The fragility of glass capillaries makes difficult the microinjection of fish eggs and embryos, which are usually protected by a hard outer shell, called the chorion. In this study, we introduce a new electromechanical approach, based on the electropiercing of fish eggs with a stationary needle electrode. The electropiercing setup consists of two asymmetric electrodes, including a μm-scaled nickel needle placed opposite to a mm-scaled planar counter-electrode. A fish egg is immersed in low-conductivity solution and positioned between the electrodes. Upon application of a short electric pulse of sufficient field strength, the chorion is electroporated and the egg is attracted to the needle electrode by positive dielectrophoresis. As a result, the hard chorion and the subjacent yolk membrane are impaled by the sharp electrode tip, thus providing direct access to the egg yolk plasma. Our experiments on early-stage medaka fish embryos showed the applicability of electro-microinjection to fish eggs measuring about 1 mm in diameter. We optimized the electropiercing of medaka eggs with respect to the field strength, pulse duration, and conductivity of bathing medium. We microscopically examined the injection of dye solution into egg yolk and the impact of electropiercing on embryos' viability and development. We also analyzed the mechanisms of electropiercing in comparison with the conventional mechanical microinjection. The new electropiercing method has a high potential for automation, e.g., via integration into microfluidic devices, which would allow a large-scale microinjection of fish eggs for a variety of applications in basic research and aquaculture.

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“…13 and 15 in Ref. 32). According to Tan et al, 37 a medaka egg experiences a critical puncture force of F p % 12.5 mN at the moment of chorion breakage, i.e., at a deformation of $400 lm.…”

Section: A Mechanical Piercing Of Medaka Eggs With a Nickel Needlementioning

confidence: 89%

mentioning

confidence: 99%

Electro-microinjection of fish eggs with an immobile capillary electrode

et al. 2015

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“…The study of cell membranes is highly important in biomedical research. It can configure the functional access in cell membranes, for example the ion channel activity in membrane surfaces, and which is an important prerequisite in drug discovery studies [76]. …”

Section: Dep Applications In Biomedical Sciencesmentioning

confidence: 99%

Dielectrophoresis for Biomedical Sciences Applications: A Review

Rahman

Ibrahim

Yafouz

2017

Sensors

170

155

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Dielectrophoresis (DEP) is a label-free, accurate, fast, low-cost diagnostic technique that uses the principles of polarization and the motion of bioparticles in applied electric fields. This technique has been proven to be beneficial in various fields, including environmental research, polymer research, biosensors, microfluidics, medicine and diagnostics. Biomedical science research is one of the major research areas that could potentially benefit from DEP technology for diverse applications. Nevertheless, many medical science research investigations have yet to benefit from the possibilities offered by DEP. This paper critically reviews the fundamentals, recent progress, current challenges, future directions and potential applications of research investigations in the medical sciences utilizing DEP technique. This review will also act as a guide and reference for medical researchers and scientists to explore and utilize the DEP technique in their research fields.

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Cell adhesion promotion strategies for signal transduction enhancement in microelectrode array in vitro electrophysiology: An introductory overview and critical discussion

Blau

2013

Current Opinion in Colloid & Interface Science

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Prototype for Automatable, Dielectrophoretically-Accessed Intracellular Membrane–Potential Measurements by Metal Electrodes

Cited by 6 publications

References 65 publications

Electro-microinjection of fish eggs with an immobile capillary electrode

Electro-microinjection of fish eggs with an immobile capillary electrode

Dielectrophoresis for Biomedical Sciences Applications: A Review

Cell adhesion promotion strategies for signal transduction enhancement in microelectrode array in vitro electrophysiology: An introductory overview and critical discussion

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