Cell adhesion promotion strategies for signal transduction enhancement in microelectrode array in vitro electrophysiology: An introductory overview and critical discussion

Blau, Axel

doi:10.1016/j.cocis.2013.07.005

Cited by 89 publications

(69 citation statements)

References 140 publications

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“…15-045). Cells are dissociated using 5% trypsin-EDTA for 15 mins prior to trituration and then are plated at 1,800 cells/mm 2 on multi-electrode arrays (MEAs, MultiChannel systems, Reutlingen, Germany) coated with poly-L-lysine (P4707, Sigma-Aldrich, St. Louis, MO, USA) [30,31]. Culture of hippocampal cells for 2 to 3 weeks is found to provide tightly synchronized periodic bursting, which is advantageous for distinguishing spontaneous activity from optically evoked activity [31,32].…”

Section: Cell Culturementioning

confidence: 99%

Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Eom

Hwang

et al. 2016

Biomed. Opt. Express

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Despite a potential of infrared neural stimulation (INS) for modulating neural activities, INS suffers from limited light confinement and bulk tissue heating. Here, a novel methodology for an advanced optical stimulation is proposed by combining near-infrared (NIR) stimulation with gold nanorods (GNRs) targeted to neuronal cell membrane. We confirmed experimentally that in vitro and in vivo neural activation is associated with a local heat generation based on NIR stimulation and GNRs. Compared with the case of NIR stimulation without an aid of GNRs, combination with celltargeted GNRs allows photothermal stimulation with faster neural response, lower delivered energy, higher stimulation efficiency and stronger behavior change. Since the suggested method can reduce a requisite radiant exposure level and alleviate a concern of tissue damage, it is expected to open up new possibilities for applications to optical neuromodulations for diverse excitable tissues and treatments of neurological disorders. Hollenberg, M.-E. Lee, and E. Haber, "Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines," Circ. Res. 82(8), 845-851 (1998). 37. S. Tandon, N. Kambi, and N. Jain, "Overlapping representations of the neck and whiskers in the rat motor cortex revealed by mapping at different anaesthetic depths," Eur. J. Neurosci. 27(1), 228-237 (2008). 38. M. Brecht, M. Schneider, B. Sakmann, and T. W. Margrie, "Whisker movements evoked by stimulation of single pyramidal cells in rat motor cortex," Nature 427(6976), 704-710 (2004).

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Section: Cell Culturementioning

confidence: 99%

Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Eom

Hwang

et al. 2016

Biomed. Opt. Express

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“…Prior to cell seeding, the MEA was coated with the polypeptide poly-d-lysine (PDL) and the ECM protein laminin, known to improve both cell adhesion and neurite outgrowth. [16,[18][19][20] Without any coating, cells did not survive past 5 days in vitro (DIV5) ( Figure S2, Supporting Information).…”

Section: Mea Design and Growth Of Cortical Cellsmentioning

confidence: 99%

Neurospheres on Patterned PEDOT:PSS Microelectrode Arrays Enhance Electrophysiology Recordings

et al. 2017

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Microelectrode arrays (MEAs) are a versatile diagnostic tool to study neural networks. Culture of primary neurons on these platforms allows for extracellular recordings of action potentials. Despite many advances made in the technology to improve such recordings, the recording yield on MEAs remains sparse. Here, enhanced recording yield is shown induced by varying cell densities on poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonate)‐coated MEAs. It is demonstrated that high cell densities (900 cells mm−2) of primary cortical cells increase the number of recording electrodes by 53.1% ± 11.3%, compared with low cell densities (500 cells mm−2) with 6.3% ± 1.4%. To further improve performance, 3D clusters known as neurospheres are cultured on the MEAs, significantly increasing single unit activity recordings. Extensive spike sorting is performed to analyze the unit activity recording multiple neurons with a single microelectrode. Finally, patterning of polyethylene glycol diacrylate through laser ablation is demonstrated, as a means to more precisely confine neurospheres on top of the electrodes. The possibility of recording single neurons with multiple neighboring electrodes is shown. Overall, a total recording yield of 21.4% is achieved, with more than 90% obtained from electrodes with neurospheres, maximizing the functionality of these planar MEAs as effective tools to study pharmacology‐based effects on neural networks.

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“…Variations in the chemical composition of material surfaces have been shown to affect a broad range of cell properties, including adhesion [45][46][47][48] , spreading 46,49 , migration 46,50 , proliferation 51 and differentiation. [51][52][53] Cell responses have been related to both specific (e.g.…”

Section: Chemicalmentioning

confidence: 99%

“…galactose) have been used to control cell behaviour. 47,59 The fibronectin derived peptide sequence RGD has received particular attention to promote integrin mediated cell adhesion to artificial surfaces. …”

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confidence: 99%

Regulation of Stem Cell Fate by Nanomaterial Substrates

Mashinchian

Turner

Dalby

et al. 2015

Nanomedicine (Lond.)

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Major design aspects for novel biomaterials are driven by the desire to mimic more varied and complex properties of a natural cellular environment with man-made materials. The development of stimulus responsive materials makes considerable contributions to the effort to incorporate dynamic and reversible elements into a biomaterial. This is particularly challenging for cell-material interactions that occur at an interface (biointerfaces); however, the design of responsive biointerfaces also presents opportunities in a variety of applications in biomedical research and regenerative medicine. This review will identify the requirements imposed on a responsive biointerface and use recent examples to demonstrate how some of these requirements have been met. Finally, the next steps in the development of more complex biomaterial interfaces, including multiple stimuli responsive surfaces, surfaces of 3D objects and interactive biointerfaces will be discussed.

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Cell adhesion promotion strategies for signal transduction enhancement in microelectrode array in vitro electrophysiology: An introductory overview and critical discussion

Cited by 89 publications

References 140 publications

Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Neurospheres on Patterned PEDOT:PSS Microelectrode Arrays Enhance Electrophysiology Recordings

Regulation of Stem Cell Fate by Nanomaterial Substrates

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