Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Eom, Kyungsik; Im, Changkyun; Hwang, Seoyoung; Eom, Seyoung; Kim, Tae Seong; Jeong, Hae Sun; Kim, Kyung Hwan; Byun, Kyung Min; Jun, Sang Beom; Kim, Sung June

doi:10.1364/boe.7.001614

Cited by 28 publications

(29 citation statements)

References 41 publications

(69 reference statements)

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“…For example, the integrity of the coil insulation was tested regularly by measuring the impedance to ground; values were typically ~1 gigohm and were always greater than 200 megohms, thereby eliminating the possibility of direct electric stimulation contributing to observed responses. We also monitored the temperature in the bath as well as in the surrounding brain tissue during magnetic stimulation and observed increases of less than 1°C, well below the threshold for thermal activation of neurons ( 36 – 38 ). Capacitive currents can be transmitted through the coil insulation and have previously been shown to be effective for neuronal activation ( 39 ).…”

Section: Resultsmentioning

confidence: 99%

Implantable microcoils for intracortical magnetic stimulation

et al. 2016

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Section: Resultsmentioning

confidence: 99%

Implantable microcoils for intracortical magnetic stimulation

et al. 2016

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“…Recently, the manipulation of cellular activity, such as light‐mediated neuromodulation techniques, has become more popular in physiological and clinical applications, because its high spatial and temporal resolution deliver real benefits to neuroscience research . For example, as shown in Figure c, the reversible inhibition of neural spiking activity in a localized area was achieved via plasmonic gold nanorod‐mediated photothermal stimulation under NIR illumination .…”

Section: Thermal Optofluidic Applicationsmentioning

confidence: 99%

Thermal Optofluidics: Principles and Applications

Chen

Loo

Wang

et al. 2019

Advanced Optical Materials

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Thermal optofluidics is an emerging field that promises to create numerous research and application opportunities in biophysics, biochemistry, and clinical biology. Innovation in plasmonic optics has led to the development of various invaluable tools in the fields of biosensing and microfluidic manipulation. The optothermal effect originates from light–matter interactions during photon–phonon conversion, which can lead to micro‐ or nanoscale inhomogeneities in the thermal distribution. This further induces a series of hydrodynamic phenomena such as natural convection, Marangoni convection, thermophoresis, the electrolyte Seebeck effect, depletion forces, and interfacial effects in colloidal particles. Light–matter interactions are particularly important for three aspects of microfluidics, namely the motion of colloidal particles, fluidic actuation, and biochemical reactions. This review first systematically elucidates the role of both nanoscale plasmonic thermal generation and heat‐induced fluidic motion in optofluidic microsystems. Then, recent state‐of‐the‐art thermal optofluidic applications of the above‐listed three aspects are presented. The paper aims to provide an insightful reference for future research in optofluidic biochemical systems.

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“…The photothermal effect of nanoparticles has been reported to successfully modulate neurons mainly in vitro 9,[11][12][13]17,[36][37][38][39][40] . Two potential stimulation mechanisms were proposed, one through the increase of temperature, with highest temperature often found in the range of 50 °C to 70 °C 17,38 , and another through an optocapacitive stimulation determined by the rate of temperature change [13][14][15] .…”

Section: Successful In Vivo Activation Through Pans Directly Injectedmentioning

confidence: 99%

“…Nanostructures target neuron membrane locally, convert and amplify the external excitation to local stimuli, offering new interfaces as promising alternative neural stimulation approaches. Gold nanoparticles and nanorods were studied for photothermal neural stimulation in vitro [9][10][11][12] . Gold nanoparticles and carbon nanotubes were also used for photothermal-driven optocapacitive stimulation in vitro [13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Neural Stimulation in vitro and in vivo by Photoacoustic Nanotransducers

Huang

Jiang²,

Luo

et al. 2020

Preprint

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Neuromodulation is an invaluable approach for study of neural circuits and clinical treatment of neurological diseases. Here, we report semiconducting polymer nanoparticles based photoacoustic nanotransducers (PANs) for neural stimulation. Our PANs strongly absorb light in the near-infrared second window and generate localized acoustic waves. PANs can also be surfacemodified to selectively bind onto neurons. PAN-mediated activation of primary neurons in vitro is achieved with ten 3-nanosecond laser pulses at 1030 nm over a 3 millisecond duration. In vivo neural modulation of mouse brain activities and motor activities is demonstrated by PANs directly injected into brain cortex. With millisecond-scale temporal resolution, sub-millimeter spatial resolution and negligible heat deposition, PAN stimulation is a new non-genetic method for precise control of neuronal activities, opening potentials in non-invasive brain modulation.. then utilized the magneto-thermal effect of the paramagnetic nanoparticles to activate these channels 18 . In these studies, significant local temperature rise, exceeding the thermal threshold of the ion channels, e.g. 43 ˚C in the case of TRPV 1, was observed, thus raising concerns over safety of thermally activated brain stimulation. The Khizroev group used the magneto-electric nanoparticles under an applied magnetic field to perturb the voltage-sensitive ion channels for neuron modulation 19 . Notably, these magnetic stimuli-based techniques deliver a spatial precision relying on the confinement of the magnetic field, which is on the millimeter to centimeter scale.New technologies and concepts are still sought to achieve non-invasive, genetic free and precise neural stimulation.Here, we report the development and application of photoacoustic nanotransducers (PANs) to enable non-genetic neural stimulation in cultured primary neurons and in live brain (Figure 1a).Our PANs, based on synthesized semiconducting polymer nanoparticles, efficiently generate localized ultrasound by an optoacoustic process upon absorption of nanosecond pulsed light in the NIR-II window (1000 nm to 1700 nm) (Figure 1b). The NIR-II light has the capability of centimeter-deep tissue penetration 20,21 , which is beyond the reach of visible light currently used in optogenetics. We further modified the PAN surface for non-specific binding to neuronal membrane and specific targeting of mechanosensitive ion channels, respectively. We showed that upon excitation at 1030 nm PANs on the neuronal membrane successfully activated rat cortical neurons, confirmed by real time fluorescence imaging of GCaMP. We then demonstrated in vivo motor cortex activation and invoked subsequent motor responses through PANs directly injected into a mouse living brain. Importantly, the heat generated by the ns laser pulses is confined inside the PAN, resulting in a transient temperature rise during the photoacoustic process, evident by COMSOL simulations. Collectively, our finding shows photoacoustic nanotransducers as a new Collectively, we have ...

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Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Cited by 28 publications

References 41 publications

Implantable microcoils for intracortical magnetic stimulation

Implantable microcoils for intracortical magnetic stimulation

Thermal Optofluidics: Principles and Applications

Neural Stimulation in vitro and in vivo by Photoacoustic Nanotransducers

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