Dielectrophoresis for Biomedical Sciences Applications: A Review

Rahman, Nurhaslina Abd; Ibrahim, Fatimah; Yafouz, Bashar

doi:10.3390/s17030449

Cited by 156 publications

(147 citation statements)

References 202 publications

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“…Moreover, in a previous study, we demonstrated that the method that we proposed based on negative DEP has high sensitivity that enables detection of as little as 80 molecules, which corresponds to 13 attomolars, per PM combined with the high specificity of an immunoassay with monoclonal antibodies [15,16]. DEP is a force produced by an electric field gradient in dielectric particles [17][18][19][20][21]. Negative DEP is a force produced by an electric field gradient to dielectric particles that is opposite to the direction of the electric field gradient.…”

Section: Introductionmentioning

confidence: 98%

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

et al. 2018

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Abstract:We show that negative dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism of a biosensor for the diagnosis and prognosis of pancreatic cancer using the biomarker CA 19-9. A substantial change in the negative DEP force applied to functionalized polystyrene microspheres (PM) was observed with respect to both the concentration level of the pancreatic cancer biomarker CA 19-9 and the frequency of the electric field produced by a pearl shaped interdigitated gold micro-electrode. The velocity of repulsion of a set of PM functionalized to a monoclonal antibody to CA 19-9 was calculated for several concentration cutoff levels of CA 19-9, including 0 U/mL and 37 U/mL, at the frequency range from 0.5 to 2 MHz. The velocity of repulsion of the PM from the electrode was determined using a side illumination and an automated software using a real-time image processing technique that captures the Mie scattering from the PM. Since negative DEP spectroscopy is an effective transduction mechanism for the detection of the cutoff levels of CA 19-9, it has the potential to be used in the early stage diagnosis and in the prognosis of pancreatic cancer.

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Section: Introductionmentioning

confidence: 98%

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

et al. 2018

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“…DEP was first studied and named by Pohl in the 1950s and the capability for biological cell separation was demonstrated in the following decade . From then on, there was an explosive growth on the investigation of DEP‐based cell viability analysis which has wide applications in the fields of medical diagnose , bioscience , environmental biology , etc.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

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Recent advances in dielectrophoresis-based cell viability assessmentDielectrophoresis (DEP) is a non-destructive, accurate, and label-free cell manipulating technique and DEP applications have been found in various fields. Assessment of cell viability is one of the important applications and many investigations have been reported. In this paper, cell polarization and its modeling, some key parameters employed for living/dead cell separation, as well as electrode configurations are reviewed. Focus is given to the latest development of DEP devices employed for the assessment of cell viability. Experimentally determined factors for separating living/dead cells, such as the conductivity of suspending medium and the frequency of applied electric field, are summarized. The future directions and potential challenges in this field are also outlined. Theory of cell DEPThe fundamentals of DEP were systematically reviewed in the past [35,36]. In this section, we only focus on the theory concerning cellular DEP. Based on the spherical particle model, single-shell model and multi-shell model of biological cells are extended. Color online: See the article online to view Figs. 1-7 in color. J. Zhang et al. Electrophoresis 2020, 41, 917-932

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“…Recent work by Lee et al surveyed the development of DEP techniques for stem cell sorting and anticipated that DEP will accelerate optimization of protocols for stem-cell usage in regenerative medicine [32]. The relevance of the DEP separation methods is underlined in a significant number of excellent reviews published over the past decade [33][34][35][36][37][38][39]. DEP, as with other marker-free methods used in CTCs enrichment, can control cellular damage or toxicity when an optimized protocol is in place [32].…”

Section: Introductionmentioning

confidence: 99%

Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells

et al. 2019

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Circulating tumor cells (CTCs) play an essential role in the metastasis of tumors, and thus can serve as a valuable prognostic factor for malignant diseases. As a result, the ability to isolate and characterize CTCs is essential. This review underlines the potential of dielectrophoresis for CTCs enrichment. It begins by summarizing the key performance parameters and challenges of CTCs isolation using microfluidics. The two main categories of CTCs enrichment—affinity‐based and label‐free methods—are analysed, emphasising the advantages and disadvantages of each as well as their clinical potential. While the main argument in favour of affinity‐based methods is the strong specificity of CTCs isolation, the major advantage of the label‐free technologies is in preserving the integrity of the cellular membrane, an essential requirement for downstream characterization. Moving forward, we try to answer the main question: “What makes dielectrophoresis a method of choice in CTCs isolation?” The uniqueness of dielectrophoretic CTCs enrichment resides in coupling the specificity of the isolation process with the conservation of the membrane surface. The specificity of the dielectrophoretic method stems from the differences in the dielectric properties between CTCs and other cells in the blood: the capacitances of the malignantly transformed cellular membranes of CTCs differ from those of other cells. Examples of dielectrophoretic devices are described and their performance evaluated. Critical requirements for using dielectrophoresis to isolate CTCs are highlighted. Finally, we consider that DEP has the potential of becoming a cytometric method for large‐scale sorting and characterization of cells.

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Dielectrophoresis for Biomedical Sciences Applications: A Review

Cited by 156 publications

References 202 publications

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

Recent advances in dielectrophoresis‐based cell viability assessment

Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells

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