Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusion

Rokka, Veli-Matti

doi:10.1007/978-1-4939-2389-2_10

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Bionica and Toluca) [125]. Nowadays, somatic hybridization through protoplast fusion is a well refined and routinely used method in order to create Solanum hybrids with different useful properties [126,127]. Plant protoplasts are naked somatic cells from which the cell wall has been removed by enzymatic digestion, therefore these cells can be used for gene transfer, somatic hybridization [128], and more recently for targeted mutagenesis and genome research.…”

Section: Use Of Cell Fusion Between Potato Crop and Its Wild Relatives For Resilient Potatomentioning

confidence: 99%

“…Bacterial wilt resistance was transferred to advanced progenies of somatic hybrids between S. commersonii and cultivated potato, and three highly resistant clones (BC1 and BC2) were selected as breeding materials [170]. In the case of potato there are many reports of symmetric interspecific somatic hybridization between diploid wild species and potato dihaploid lines [127]. The main problem with the majority of these hybrids was the infertility, which made difficult the restoration of valuable cultivar.…”

Section: Use Of Cell Fusion Between Potato Crop and Its Wild Relatives For Resilient Potatomentioning

confidence: 99%

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Biotechnological Strategies for a Resilient Potato Crop

Rákosy-Tican¹,

Molnár²

2021

Solanum Tuberosum - A Promising Crop for Starvation Problem

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The aim of this chapter is to describe in a synthetic manner the most efficient biotechnological techniques which can be applied in potato breeding with emphasis on multiple resistance traits. To this end, most important results of all biotechnological techniques will be pointed out including new biotechnological tools of genome editing. The somatic hybridization will be the core of the presentation as the only non-GMO strategy with good results in transferring multiple resistances into potato gene pool. The chapter is presenting all data in a synthesized form and made comparisons between the existing techniques and their possible adoption in breeding in different parts of the world, depending on regulations and consumer choice. Moreover, the recently discovered value of potato as a healthy food and its possible applications in cancer treatment will be also discussed with new data on both potato and some of its wild relatives.

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Section: Use Of Cell Fusion Between Potato Crop and Its Wild Relatives For Resilient Potatomentioning

confidence: 99%

Section: Use Of Cell Fusion Between Potato Crop and Its Wild Relatives For Resilient Potatomentioning

confidence: 99%

Biotechnological Strategies for a Resilient Potato Crop

Rákosy-Tican¹,

Molnár²

2021

Solanum Tuberosum - A Promising Crop for Starvation Problem

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“…By means of somatic hybridization, several resistance genes from wild species have already been introduced into potato, including those that confer tolerance to herbicide (BINDING et al, 1982), resistance to leafroll virus (AUSTIN; BAER; HELGESON, 1985), tolerance to frost (ROKKA et al, 1998), tolerance to late blight disease (SHI et al, 2006), and resistance to insects (CHENG;SAUNDERS;SINDEN, 1995;BUTLER et al, 2011;DIAZ-MONTANO et al, 2014). Specific reviews of somatic hybridization in potato can be found in Pandey et al (2010), Cho and Park (2014) and Rokka (2015).…”

Section: Protoplast Culturementioning

confidence: 99%

“…Thus, cryopreservation is a more reliable alternative. In addition to long-term storage of potato germplasm, cryotherapy can simultaneously be applied as an alternative method for virus elimination and production of indexed plants (WANG et al, 2006;KACZMARCZYK;ROKKA;KELLER, 2011).…”

Section: In Vitro Preservation and Cryopreservationmentioning

confidence: 99%

Application of tissue culture techniques in potato

Morais¹,

Asmar²,

Silva³

et al. 2018

Biosci. J.

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Potato is the world's most important non-cereal food crop, and therefore, it is considered one of the major food sources for humankind. Its conventional propagation is asexual, by using the tuber, which allows the accumulation and dissemination of pathogens to new cultivation areas. This fact not only impairs the yield of this solanaceous plant, but also threatens the maintenance of genotypes for commercial or breeding purposes. Due to the impossibility of using botanical seed, conservation and exchange of germplasm of this species by means of conventional methods are not feasible. In all potato-producing regions, the demand for high-quality tubers has been paramount to ensure crops production. Thus, biotechnological techniques based on tissue culture are very important. Plant tissue culture offers alternative methods of propagation by in vitro techniques that provide production and multiplication of material with high sanity. Thus, this literature review summarizes the history and current situation of tissue culture techniques applied to potato crop. Besides clonal multiplication, this biotechnological tool makes available initial indexed material to breeding programs and certified seed potato, and facilitates the exchange and conservation of germplasm. For all these reasons, the use of these techniques in potato production chain directly benefits producers by providing high-quality propagules.

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Somatic Cell Genetics and Its Application in Potato Breeding

Thieme

Rákosy-Tican

2017

Compendium of Plant Genomes

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Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusion

Cited by 5 publications

References 43 publications

Biotechnological Strategies for a Resilient Potato Crop

Biotechnological Strategies for a Resilient Potato Crop

Application of tissue culture techniques in potato

Somatic Cell Genetics and Its Application in Potato Breeding

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