Somatic Cell Genetics and Its Application in Potato Breeding

Thieme, Ramona; Rákosy-Tican, Elena

doi:10.1007/978-3-319-66135-3_13

Cited by 5 publications

(8 citation statements)

References 384 publications

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“…We used somatic hybridization, as the resulting plants are not genetically modified (directive 2001/18/EC–annex 1B) 2 . The use of somatic hybridization does enable to transfer new assortments of nuclear and cytoplasmic genes into regenerated plants and to overcome sexual incompatibility barriers ( Rakosy-Tican et al, 2015 ; Thieme and Rakosy-Tican, 2017 ). Moreover, when asymmetric SHs are produced because of somatic incompatibilities ( Rakosy-Tican et al, 2015 ), only a part of the nuclear DNA from the wild parent is present in the hybrids and derived BCs.…”

Section: Discussionmentioning

confidence: 99%

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

et al. 2020

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Potato Late Blight Resistance Genes of the parent cultivar 'Delikat' in field trials. The evaluation of agronomic traits of selected BC 2 clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding Avr effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.

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Section: Discussionmentioning

confidence: 99%

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

et al. 2020

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“…The wealth of wild Solanum tuber-bearing species (∼226), which are closely related to cultivated potato, are an important reservoir of resistance genes for potato improvement (Hawkes, 1990). This is a source that might be used in combinatorial biotechnology, e.g., for breeding resistance (Rakosy-Tican, 2012; Rakosy-Tican et al, 2016; Thieme and Rakosy-Tican, 2017). Solanum chacoense Bitt.…”

Section: Introductionmentioning

confidence: 99%

New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization

et al. 2019

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As plants are sessile they need a very efficient system for repairing damage done by external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of MSH2 gene may induce a ‘mutator’ phenotype and microsatellite instability (MSI), as is demonstrated in MSH2 knock-out and silenced lines of Arabidopsis thaliana. The goal of this study was to screen for ‘mutator’ phenotypes in somatic hybrids between potato cvs. ‘Delikat’ and ‘Désirée’ and MMR deficient Solanum chacoense transformed using antisense (AS) or dominant negative mutant (DN) AtMSH2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; these mutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with ‘mutator’ phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene nptII, from the initial constructs. Here for the first time, MMR deficiency combined with somatic hybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species.

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“…One way for transferring multiple resistance genes from wild Solanum species is somatic hybridization via protoplast electrofusion. The advantages of this biotechnological approach are: (i) hybridization of sexually incompatible but related species that is impossible or difficult to realize using classical methods, (ii) the transfer of polygenes involved in resistance and/or multiple resistance traits, (iii) the somatic hybrid plants obtained through this technology are not considered as GMO in Europe (directive 2001/18/EC-annex 1B) [133]. Several studies demonstrate the possibility of transfer of the CPB resistance genes from wild relatives (S. tarnii, S. cardiophyllum and S. chacoense) to somatic hybrids and back-cross progenies (BCs) [134][135][136][137].…”

Section: Perspectives To Reduce Insecticide Usementioning

confidence: 99%

Difficulties in Potato Pest Control: The Case of Pyrethroids on Colorado Potato Beetle

Molnár

Rákosy-Tican

2021

Agronomy

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Colorado potato beetle (Leptinotarsa decemlineata, CPB) is considered one of the most persistent crop pests because it is highly adaptable and can rapidly develop insecticide resistance. Nowadays, this beetle is resistant to over 54 different insecticides. In the absence of competitive alternatives, the answer of farmers to the high degree of pest adaptability is to increase the number of pesticide treatments or apply chemicals with different modes of action. Such a strategy increases the risk of intoxication in non-target organisms and leads to environmental pollution, augmenting the carbon footprint. Furthermore, these strategies are also unsustainable and inefficient for pest management in the long-term. The time has thus come to reform existing agriculture practices, for which the implementation of an integrated pest management strategy would be a more feasible tool. Applying a sustainable pest management strategy is indispensable for a better understanding of the status and mechanisms of insecticide resistance. Effective pest management requires monitoring the resistance of pests and developing a well-programmed pesticide treatment to simultaneously reduce the insecticide selection pressure and environmental pollution. In this context, here we present a review on the difficulties of potato pest control using as a case study the resistance of CPB to pyrethroids.

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Somatic Cell Genetics and Its Application in Potato Breeding

Cited by 5 publications

References 384 publications

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization

Difficulties in Potato Pest Control: The Case of Pyrethroids on Colorado Potato Beetle

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