Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl.

Abstract

An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

“…These globules gradually grew in size, having no vascular connections with the surrounding peripheral tissues and eventually transformed into PLBs. The PLBs proliferated further to produce secondary PLBs as reported earlier (Zhao et al 2008).…”

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

“…Zhao et al (2008) reported that the majority of Dendrobium candidum PLBs produced secondary PLBs originated from cells of the interior or exterior of the embryogenic callus. Chen and Chang (2006) also observed continuous production of somatic embryos in Phalaenopsis amabilis through secondary embryogenesis directly from leaf explants.…”

“…Many authors states that PLBs are somatic embryos (Hossain et al, 2013;Lee et al, 2013), or that somatic embryogenesis is the initial step on the PLBs formation (Zhao, Wu, Feng, & Wang 2008). Through histological and histochemical studies, Lee et al (2013) demonstrated that during the early stages of PLB formation, the cells show cytological characteristics and cell wall markers similar to zygotic embryo development.…”

Establecimiento in vitro de protocormos de Prosthechea sp. bajo diferentes concentraciones de ácido naftalenacético

“…con 0,2 mg/l -1 de ANA y en Cymbidium sp. con 0,1 mg/l -1 de ANA (Ávila-Díaz et al, 2009;Zhao et al, 2008).…”

“…Debido a esta facilidad, los protocormos se han utilizado como explantes en trabajos de embriogénesis somáti-ca y organogénesis directa e indirecta, cuyas técnicas son aplicadas ampliamente en la micropropagación vegetativa (Tuong et al, 2004;Zhao et al, 2008).…”