In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

Abstract

Transverse sections (2 mm thickness) of stem-nodes from in vitro raised seedlings had morphogenic potential on semisolid and liquid Murashige and Skoog medium supplemented with cytokinins N 6 -benzyladenine (BA 4.44 μM), furfurylaminopurine (KIN 4.65 μM) and auxin α-naphthalene acetic acid (NAA 5.37 μM) individually and in combinations. The regeneration response was influenced by both the type of growth regulator and physical state of the medium. The explants produced either shoot buds on cytokinincontaining media or protocorm-like bodies (PLBs) on NAA containing media both solid and liquid. More neo-formations were produced on liquid media, especially those containing only NAA. They were formed at nodal and inter-nodal regions. The secondary buds were produced on the surface of primary PLBs. The plantlets were developed on MS medium containing banana homogenate 50 g·dm -3 . The current study is the first ever report on successful regeneration of Dendrobium chrysotoxum from stem-node segments.

A Comprehensive Review Uncovering the Challenges and Advancements in the In Vitro Propagation of Eucalyptus Plantations

“…The initiation of shoot proliferation is usually achieved by culturing explant on a semisolid medium that comprises gelling agents such as 1.5-4.0 g/L of gelrite, 4-8 g/L of agar, or 1.5-4.5 g/L of phytagel, and the pH is adjusted between 5 and 6. Also, the use of liquid media has been reported for the initiation and proliferation of nodes and shoots [74,75]. The culture of the shoot depends on the ability to encourage the development of axillary and accessory buds that are present at the base of each leaf axil.…”

A Comprehensive Review Unleashing the Challenges and Advancements in In-Vitro Propagated Eucalypt Plantations

“…The initiation and shoot proliferation is usually achieved by culturing explant on semi-media that is made up of Gelling agents such as 1.5-4.0g/ L gelrite, 4-8g/L agar, or 1.5 -4.5 g/L phytagel, and the pH is adjusted between 5-6. Also, use of liquid media has been reported for the initiation and proliferation of nodes and shoots [67,68]. The culture of the shoot depends on the ability to encourage the development of axillary and accessory buds that are present at the base of each leaf axil.…”

Efek Zat Pengatur Tumbuh NAA untuk Optimasi Pembesaran Bulbophyllum spp. secara In Vitro

“…Air kelapa dari kelapa muda yang masih segar juga merupakan sumber pengganti BAP, bahkan sedikit lebih baik dibanding BAP (Buah dan Agu-Asare 2014). Adapun pada penelitian Kaur (2017), disimpulkan bahwa kandungan karbohidrat yang tinggi pada pisang 50 g/l dapat mendukung pertumbuhan planlet yang kuat pada Coelogyne flaccida, Dendrobium chrysotoxum, maupun Spathoglottis kimballiana.…”