Protocols using detomidine and oxytocin induce ex copula ejaculation in stallions

Cavalero, Thaís Mendes Sanches; Papa, Federico; Schmith, Rúbia Alves; Scheeren, V.F.C.; Canuto, Lucas Emanuel Ferreira; Gobato, Mariana Luiza Mezzena; Rodrigues, Luciana; Freitas-Dell’Aqua, C.P.

doi:10.1016/j.theriogenology.2019.08.024

Cited by 12 publications

(17 citation statements)

References 19 publications

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“…The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg; Dormium, Agener Union) and oxytocin (20 IU; UCBVet Animal Health) and followed the procedures described by Cavalero et al. (2019). Immediately after collection, the gel‐free volume was measured in a graduated tube.…”

Section: Methodsmentioning

confidence: 99%

“…Sperm kinetics were evaluated by CASA (IVOS‐HTM 12; Hamilton Thorne Research). Total motility (TM [%]), progressive motility (PM [%]) and rapid sperm percentage (RAP [%]) were calculated using standard settings for equine sperm (Cavalero et al., 2019). Semen was stored at 5°C for 24 hr in a passive cooling system (BotuFlex; Botupharma).…”

Section: Methodsmentioning

confidence: 99%

“…Semen was collected either (a) through an artificial vagina (AV; Botucatu model), with the stallion mounting a dummy in the presence of an oestrous mare (n = 3), or (b) through pharmacologically induced ejaculation (n = 3). The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg; Dormium, Agener Union) and oxytocin (20 IU; UCBVet Animal Health) and followed the procedures described by Cavalero et al (2019). Immediately after collection, the gelfree volume was measured in a graduated tube.…”

Section: Materials S and Me Thodsmentioning

confidence: 99%

“…The vesicular glands secrete the final portion of the ejaculate (e.g., gel fraction). Pharmacologically induced ejaculation reduces the level of seminal plasma in the ejaculate (Cavalero et al., 2019); therefore, this method may benefit sperm quality of stallions with seminal vesiculitis (Varner & Schumacher, 2011). To this end, the present study evaluated the characteristics of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis

Cavalero

Segabinazzi

Scheeren

et al. 2020

Reprod Domestic Animals

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The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10-year-old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Materials S and Me Thodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis

Cavalero

Segabinazzi

Scheeren

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The administration of xylazine alone for ex copula ejaculation in horses has been reported in both research (11,12) and clinical settings (5). In a study involving 28 stallions (12 horses and 16 ponies), xylazine was administered intravenously at a dose rate of 0.7 mg/kg and the stallions were left undisturbed for 30 min (11). Each stallion underwent a trial with sexual prestimulation and a trial without sexual prestimulation.…”

Section: Xylazine Alone Protocolmentioning

confidence: 99%

Pharmacologically Induced Ex Copula Ejaculation in Horses and Donkeys

2021

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Pharmacologically induced ex copula ejaculation is a method used for collection of semen when the traditional methods of semen collection are not feasible. Common indications for this method include health issues that either preclude the physical act of mating or result in impaired erection and ejaculation. The method also offers an alternative when there is a lack of equipment and facilities required for semen collection using the conventional artificial vagina method. A variety of pharmacological protocols for ex copula ejaculation in stallions have been evaluated in both research and clinical settings with no serious side-effects reported. In general, these protocols included tricyclic antidepressants, alpha-2 adrenergic receptor agonists, and smooth muscle stimulators, either individually or in combination. Although there is a lot of variation in the ejaculatory rates among studies, a combination of imipramine and xylazine appears to be an effective option for inducing ejaculation in stallions. In cases where this protocol is not successful, collections should be reattempted using a combination of imipramine, detomidine, and oxytocin. Regardless of the protocol used, a quiet environment with minimal disturbance is associated with a better outcome. In contrast to the body of literature available on pharmacologically induced ex copula ejaculation in horses, only a few studies have been published so far on this topic in donkeys. Further studies are warranted to investigate whether pharmacologically induced ex copula ejaculation is an effective method of semen collection in jacks and to compare different pharmacological protocols for inducing ejaculation in jacks.

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Alternative Protocol Using Imipramine, Detomidine, and Oxytocin for Semen Collection in Stallion with Ejaculatory Dysfunction

Cavalero

Segabinazzi

Scheeren

et al. 2020

Journal of Equine Veterinary Science

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Protocols using detomidine and oxytocin induce ex copula ejaculation in stallions

Cited by 12 publications

References 19 publications

Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis

Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis

Pharmacologically Induced Ex Copula Ejaculation in Horses and Donkeys

Alternative Protocol Using Imipramine, Detomidine, and Oxytocin for Semen Collection in Stallion with Ejaculatory Dysfunction

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