Protocol of Mensuration to Avaliation of Indicators of Somatic Development of Wistar Rats

Abstract

SUMMARY:Evaluations of weight and cephalic perimeter are very used to study the growth and development in human beings. Similar measures, called murinometrics, have been an efficient resource to study the effects of nutritional and pharmacologic manipulations on the somatic development in rat. The aim of this study is to describe a method of evaluation which offers a higher accuracy in the collect of data in the study of somatic development in Wistar rat. Measurements were realized in latero-lateral axis of skull; Antero-posterior axis of skull; Longitudinal axis; Length of tail in 60 wistar rat during breastfeeding period. The most suitable method of measurement respects predetermined anatomic points, the use of the suitable scapus of pachymeter as well as its accuracy.

Early physical activity minimizes the adverse effects of a low-energy diet on growth and development parameters

“…The lack of nutrients and/or energy in developing organisms is usually demonstrated by growth deficit. 3,25 The diet used in our study also results in adverse consequences similar to those observed in the low-protein model. These findings have important implications for the optimal use of protein which is based on its quality and quantity along with an adequate energy intake ( protein:energy rate).…”

“…Measurement of somatic growth 25 during lactation (tail length (TL), lateral-lateral axis of the skull (LLAS), anterior-posterior axis of the skull (APAS), and longitudinal axis (LA)) was performed every 3 days using a Stainless Hardened (Jomarca, Brazil) digital caliper with an accuracy of 0.01 mm from postnatal day 1 to 21. 25 Daily measurements of reflex maturation during lactation and characterized according to the protocol of Fox 26 is shown in Chart 1: recovery position, vibrissae placing (VP), cliff aversion (CA), negative geotaxis (NG), auditory startle response (AS), and free-fall righting (FR). For each reflex, the day of consolidation of its response was recorded, which was considered as the first day of the sequence of three consecutive days of a fullblown reflex response.…”

“…The LE diet used in this study resulted in reduced tail and body length, which are parameters associated with the growth of the animal. According to Silva et al 25 the evaluation of body axes examines the variability of the effects of malnutrition in developing rats. The lack of nutrients and/or energy in developing organisms is usually demonstrated by growth deficit.…”

Effects of a Westernized Diet on the Reflexes and Physical Maturation of Male Rat Offspring During the Perinatal Period

“…The specific rate of weight gain was calculated employing the formula SWG = [(weight at day 21-weight at day 1)/ (weight at day 1 9 total number of days) [24]. Measurements of tail length (distance from the tip to the base of the tail), latero-lateral axis of the skull (distance between the ear holes) and the length of the anteroposterior axis of the skull (distance between the muzzle and head-neck joint) were obtained using digital calipers (Starrett Ò ), with an accuracy of 0.01 mm [25].…”

Malnutrition and Somatic Development: Application of Protocol of Mensuration to Evaluation of Indicators of Wistar Rats

“…However, it was observed some difficulty to establish more accurate parameters in the collect of data in our studies. In order to find more objective standard for the measurement of indicators of somatic development it was proposed the protocol of Silva et al (2005). The protocol considers the suitable use of the scapus of the pachymeter, suggesting the use of an instrument with better degree of accuracy and defining anatomic points as bases for the measurements.…”